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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0964 [new locus tag: SACOL_RS04945 ]
  • pan locus tag?: SAUPAN003068000
  • symbol: argG
  • pan gene symbol?: argG
  • synonym:
  • product: argininosuccinate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0964 [new locus tag: SACOL_RS04945 ]
  • symbol: argG
  • product: argininosuccinate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 966915..968120
  • length: 1206
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAAGAGAAAATTGTTTTAGCATATTCAGGAGGACTAGATACAAGTGTGGCCGTTCAA
    TGGCTTATCGACAAAGGATACGACGTTGTAGCTTGTTGCCTAGATGTTGGTGAAGGTAAA
    GATTTAGACATCGTTTATAAAAAAGCTTTAGATATGGGAGCAGTTGAATGTCATATTATT
    GATGCAACAAAAGAATTTAGTGATGAGTATGTAAGTTATGCAATCAAAGGAAATTTAATG
    TATGAAAATGCATATCCATTAGTTTCAGCTTTATCAAGACCATTAATCGCTAAAAAATTA
    GTAGAGATTGCTGAGAAAACAAATTCAGTAGGTATTGCACATGGTTGTACTGGTAAAGGG
    AATGACCAAGTACGTTTCGAAGTTGCCATTAAAGCATTAAACCCATCATTGAAAGCATTC
    GCACCTGTACGTGAGTGGGCATGGAGTCGTGAAGAAGAAATCGATTATGCAATTAAACAT
    AATATCCCTGTATCAATCAACCATGATTCACCTTATTCTATCGATCAAAATCTATGGGGC
    AGAGCGAATGAATGTGGTATTTTAGAAGATCCTTATGCTGCGCCACCAGAGGATGCGTTC
    GATCTAACAAATGCTTTAGAAGAAACACCAGATACTGCTGATGAAATCATTTTAACGTTT
    GATAAAGGCATCCCAGTTCAAATTGATGGCAAAACATATGAATTAGACGATTTAATTTTA
    ACGTTGAATGCATTAGCTGGTAAGCATGGTATCGGAAGAATTGACCATGTAGAAAATAGA
    CTTGTAGGTATCAAATCAAGAGAAATTTATGAGGCACCTGCTGCAGAAGTTATTTTAAAA
    GCGCATAAAGCATTAGAAACGATTACGTTAACGAAAGATGTCGCACACTTTAAACCAATC
    ATTGAGAAGCAATTTGCTGAACAACTATACAATGGACTTTGGTTCTCACCTTTAACTGAT
    AGCTTGAAATTATTTATTGATAGTACTCAGCAATACGTAAGTGGTGATGTCAGAATTAAA
    TTATTCAAAGGTAATGCCATCGTGAATGGTAGAAAATCACCTTACACATTATATGATGAA
    AAATTAGCAACTTATACAAAAGAAGATGCATTTAATCAAGACGCTGCTGTTGGCTTTATC
    GATATCTATGGTTTACCTACTCAAGTAAATGCGATGTTGCATGGAGGCTATAGCAATGAG
    CAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0964 [new locus tag: SACOL_RS04945 ]
  • symbol: ArgG
  • description: argininosuccinate synthase
  • length: 401
  • theoretical pI: 4.61504
  • theoretical MW: 44455.1
  • GRAVY: -0.182544

Function[edit source | edit]

  • reaction:
    EC 6.3.4.5?  ExPASy
    Argininosuccinate synthaseATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyargininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 524.1)
    Unknown functionGeneralqueuosine biosynthesis protein QueC (TIGR00364; HMM-score: 14.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 13.1)
    Hypothetical proteinsConservedTIGR00268 family protein (TIGR00268; HMM-score: 12.7)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 12.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminetRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 12.5)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine Biosynthesis extended Argininosuccinate synthase (EC 6.3.4.5) 
    MiscellaneousMiscellaneous - no subcategoryEC 6.3.4.- Ligases that form carbon-nitrogen bonds Argininosuccinate synthase (EC 6.3.4.5) 
  • PFAM:
    HUP (CL0039) Arginosuc_synth; Arginosuccinate synthase (PF00764; HMM-score: 556.4)
    tRNA_Me_trans; tRNA methyl transferase (PF03054; HMM-score: 16.9)
    QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 14.2)
    ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 11.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL1734(gapA2)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 28
    • Cmax: 0.12
    • Cmax_pos: 68
    • Smax: 0.18
    • Smax_pos: 26
    • Smean: 0.117
    • D: 0.121
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDIVYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVEIAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKHNIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIILTFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAEVILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVSGDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAMLHGGYSNEQ

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: ArgR, CodY
    ArgR (TF) important in Arginine biosynthesis, Arginine degradationRegPrecise
    CodY (TF) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]