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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1001 [new locus tag: SACOL_RS05115 ]
  • pan locus tag?: SAUPAN003163000
  • symbol: trpS
  • pan gene symbol?: trpS
  • synonym:
  • product: tryptophanyl-tRNA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1001 [new locus tag: SACOL_RS05115 ]
  • symbol: trpS
  • product: tryptophanyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1009158..1010147
  • length: 990
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGAGACATTATTTTCAGGCATCCAACCTAGTGGAATTCCTACTATTGGAAATTATATT
    GGCGCACTAAAACAATTTGTTGATGTGCAAAATGACTATGATTGTTATTTCTGTATCGTA
    GATCAACATGCAATTACAATGCCACAAGATCGTTTAAAATTACGTAAACAGACCAGACAA
    TTAGCAGCGATTTATTTAGCTTCTGGTATAGATCCAGACAAAGCAACATTGTTCATACAA
    TCTGAAGTCCCTGCACACGTACAAGCAGGATGGATGTTAACTACGATTGCTTCTGTTGGA
    GAATTAGAGCGTATGACGCAATACAAAGATAAAGCTCAGAAAGCAGTTGAAGGTATACCT
    GCTGGTCTATTAACATATCCACCTTTAATGGCAGCTGATATTGTTCTTTACAATACTAAT
    ATCGTTCCAGTTGGAGATGACCAAAAGCAGCATATCGAATTGACTCGTAACCTTGTAGAT
    AGATTTAATAGTCGCTATAATGATGTGCTTGTGAAACCTGAAATTCGTATGCCTAAAGTT
    GGTGGACGTGTCATGAGTTTACAAGACCCAACAAGAAAAATGAGTAAGAGTGATGATAAT
    GCTAAAAACTTCATTTCATTATTAGACGAGCCGAATGTTGCAGCTAAAAAAATTAAAAGC
    GCAGTAACTGATTCAGATGGTATTATTAAATTTGATCGTGACAACAAGCCAGGTATAACA
    AATTTAATTTCAATATACGCTGGATTAACAGACATGCCAATTAAAGATATTGAGGCAAAA
    TATGAGGGCGAAGGTTATGGTAAATTTAAAGGTGACCTTGCTGAAATAGTTAAAGCATTT
    TTAGTAGAATTCCAAGAAAAATACGAAAGTTTCTATAACTCAGATAAACTTGATGATATT
    TTAGATCAAGGTAGAGATAAAGCACACAAAGTTTCATTTAAAACTGTCAAAAAAATGGAA
    AAAGCGATGGGTTTAGGACGTAAGAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    990

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1001 [new locus tag: SACOL_RS05115 ]
  • symbol: TrpS
  • description: tryptophanyl-tRNA synthetase
  • length: 329
  • theoretical pI: 8.14756
  • theoretical MW: 36909.2
  • GRAVY: -0.387538

Function[edit source | edit]

  • reaction:
    EC 6.1.1.2?  ExPASy
    Tryptophan--tRNA ligaseATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationtryptophan--tRNA ligase (TIGR00233; EC 6.1.1.2; HMM-score: 362.5)
  • TheSEED:  
    Protein MetabolismProtein biosynthesistRNA aminoacylation, Trp Tryptophanyl-tRNA synthetase (EC 6.1.1.2) 
  • PFAM:
    HUP (CL0039) tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 266.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL1587(efp)elongation factor P  [1] (data from MRSA252)
    SACOL2092(murAA)UDP-N-acetylglucosamine 1-carboxyvinyltransferase  [1] (data from MRSA252)
    SACOL0841(pgm)phosphoglyceromutase  [1] (data from MRSA252)
    SACOL1011(ppnK)inorganic polyphosphate/ATP-NAD kinase  [1] (data from MRSA252)
    SACOL1091(ptsH)phosphocarrier protein HPr  [1] (data from MRSA252)
    SACOL0731LysR family transcriptional regulator  [1] (data from MRSA252)
    SACOL1437CSD family cold shock protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.132
    • Ymax_pos: 23
    • Cmax: 0.156
    • Cmax_pos: 23
    • Smax: 0.221
    • Smax_pos: 21
    • Smean: 0.097
    • D: 0.119
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHAITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGELERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVDRFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKIKSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEIVKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [2] [3] [4]
    quantitative data / protein copy number per cell: 1084 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • trpS no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 22.29 h [6]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Suzanne E Dale, Amanda Doherty-Kirby, Gilles Lajoie, David E Heinrichs
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
Infect. Immun.: 2004, 72(1);29-37
[PubMed:14688077] [WorldCat.org] (P p)