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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1010 [new locus tag: SACOL_RS05160 ]
  • pan locus tag?: SAUPAN003177000
  • symbol: relA1
  • pan gene symbol?: relQ
  • synonym:
  • product: GTP pyrophosphokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1010 [new locus tag: SACOL_RS05160 ]
  • symbol: relA1
  • product: GTP pyrophosphokinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1017791..1018426
  • length: 636
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAATCAATGGGATCAGTTCTTAACACCTTATAAGCAAGCGGTTGATGAGTTGAAAGTG
    AAACTTAAAGGCATGCGCAAACAATATGAAGTTGGTGAACAAGCGTCGCCAATAGAATTT
    GTTACTGGTCGTGTTAAACCAATCGCTAGTATTATAGATAAGGCAAACAAACGACAAATA
    CCATTTGATAGGTTAAGAGAAGAAATGTACGATATCGCTGGTTTAAGAATGATGTGCCAA
    TTTGTTGAAGATATTGATGTTGTCGTCAATATTTTAAGACAAAGAAAAGATTTTAAAGTA
    ATTGAAGAACGAGATTATATTCGTAACACTAAAGAAAGTGGTTACCGCTCGTATCATGTC
    ATTATTGAATATCCAATTGAAACATTACAAGGCCAAAAATTTATATTGGCTGAGATTCAG
    ATTCGTACATTAGCAATGAATTTCTGGGCAACGATTGAACATACTTTACGATATAAATAT
    GATGGTGCTTATCCGGATGAAATTCAACATCGTTTGGAAAGAGCGGCAGAAGCAGCGTAT
    TTACTTGATGAAGAGATGTCTGAAATTAAAGATGAAATTCAGGAAGCTCAAAAATATTAC
    ACGCAAAAACGTTCTAAAAAACATGAAAATGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    636

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1010 [new locus tag: SACOL_RS05160 ]
  • symbol: RelA1
  • description: GTP pyrophosphokinase
  • length: 211
  • theoretical pI: 6.16491
  • theoretical MW: 25238.7
  • GRAVY: -0.72654

Function[edit source | edit]

  • reaction:
    EC 2.7.6.5?  ExPASy
    GTP diphosphokinaseATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
  • TIGRFAM:
    Cellular processesCellular processesAdaptations to atypical conditionsRelA/SpoT family protein (TIGR00691; HMM-score: 36)
  • TheSEED:  
    CBSS-222523.1.peg.1311 GTP pyrophosphokinase (EC 2.7.6.5) 
  • PFAM:
    NTP_transf (CL0260) RelA_SpoT; Region found in RelA / SpoT proteins (PF04607; HMM-score: 124.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.101
    • Ymax_pos: 25
    • Cmax: 0.107
    • Cmax_pos: 52
    • Smax: 0.131
    • Smax_pos: 12
    • Smean: 0.099
    • D: 0.1
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGRVKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIEERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKYDGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 119 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Tobias Geiger, Benjamin Kästle, Fabio Lino Gratani, Christiane Goerke, Christiane Wolz
Two small (p)ppGpp synthases in Staphylococcus aureus mediate tolerance against cell envelope stress conditions.
J. Bacteriol.: 2014, 196(4);894-902
[PubMed:24336937] [WorldCat.org] [DOI] (I p)