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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1032 [new locus tag: SACOL_RS05265 ]
  • pan locus tag?: SAUPAN003211000
  • symbol: SACOL1032
  • pan gene symbol?: comK1
  • synonym:
  • product: ComK family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1032 [new locus tag: SACOL_RS05265 ]
  • symbol: SACOL1032
  • product: ComK family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1042540..1043070
  • length: 531
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGTTATTCGACCAGCATTTGATGATGACGATCAAAGAAACGGTAGTGAAATAATTCGG
    TTTGACAAAACGCGTATTCAAAATCCTTTTAAAGTCCAGAAAATCATTGAACGCTCTTGC
    AAATTTTATGGTAATACTTATCTTGGCAAGAAAGCAGAGACAAACCGCATTACTGGCATT
    TCTAGTAAACCACCTATTTTACTAACACCATTATTTCCAACTTATTTTTTCCCAACACAT
    TCTGACAGACAAAATGAAAATATTTGGTTAAATATGCATTATATCGAAAGTATTAAAGAA
    TTAAAAAATCGTAAATGTAAAGTGACATTTATTAATAATGAATCAATCATTCTTCATGTT
    TCATACCACAGTTTATGGCATCAATATAACAATTCCATTTTTTACTATTACATGGTAGAT
    AAACAATCTCGCATGATATCAAAAAATCCCGACCAACCAATAGATTATAATAAAGCCACA
    TTGAATGTGTTTGAAGCATTGACACGCTATTCTTTATTTGAAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    531

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1032 [new locus tag: SACOL_RS05265 ]
  • symbol: SACOL1032
  • description: ComK family protein
  • length: 176
  • theoretical pI: 9.55605
  • theoretical MW: 21032.9
  • GRAVY: -0.647159

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Competence transcription factor 
  • PFAM:
    PLP_aminotran (CL0061) ComK; ComK protein (PF06338; HMM-score: 159.3)
    no clan definedGemin7; Gem-associated protein 7 (Gemin7) (PF11095; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.098
    • Ymax_pos: 60
    • Cmax: 0.108
    • Cmax_pos: 35
    • Smax: 0.115
    • Smax_pos: 58
    • Smean: 0.082
    • D: 0.091
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVIRPAFDDDDQRNGSEIIRFDKTRIQNPFKVQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIWLNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMISKNPDQPIDYNKATLNVFEALTRYSLFEDK

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]