From AureoWiki
Jump to: navigation, search
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1043 [new locus tag: SACOL_RS05325 ]
  • pan locus tag?: SAUPAN003234000
  • symbol: SACOL1043
  • pan gene symbol?: tarM
  • synonym:
  • product: glycosyl transferase, group 1 family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1043 [new locus tag: SACOL_RS05325 ]
  • symbol: SACOL1043
  • product: glycosyl transferase, group 1 family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1050816..1052297
  • length: 1482
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAAAAAATATTTATGATGGTACATGAGTTAGATGTGAATAAAGGTGGTATGACCTCT
    TCGATGTTCAATAGAAGTAAAGAGTTTTATGATGCGGACATACCTGCTGATATTGTTACT
    TTCGATTACAAAGGAAACTATGATGAAATTATTAAAGCTTTGAAAAAACAAGGTAAAATG
    GATCGAAGAACGAAAATGTATAATGTATTTGAGTATTTTAAACAAATTTCAAATAATAAA
    CATTTTAAGTCTAATAAATTGTTATATAAACATATTTCAGAAAGACTAAAAAATACGATT
    GAAATTGAAGAGAGTAAAGGTATTTCAAGATATTTTGATATAACGACTGGTACATATATT
    GCCTACATTAGAAAAAGTAAATCTGAAAAAGTGATTGATTTCTTTAAAGATAATAAACGA
    ATTGAACGGTTTAGTTTTATAGATAATAAAGTGCATATGAAGGAAACATTTAATGTAGAT
    AATAAAGTTTGTTATCAAGTATTTTATGATGAAAAGGGATACCCATATATTTCAAGGAAT
    ATTAATGCTAATAATGGTGCTGTAGGTAAAACTTATGTGTTAGTTAATAAAAAAGAATTT
    AAAAACAATTTAGCACTGTGTGTTTACTATTTAGAAAAACTAATAAAAGATTCTAAAGAT
    AGTATTATGATTTGTGATGGACCAGGGAGTTTTCCAAAAATGTTTAATACAAATCATAAA
    AATGCTCAGAAATATGGCGTTATTCATGTTAATCATCATGAAAATTTCGATGATACGGGT
    GCATTTAAAAAAAGTGAGAAATATATTATTGAGAATGCGAATAAAATTAACGGTGTAATT
    GTATTAACAGAGGCACAAAGATTAGATATTCTTAATCAATTTGATGTAGAAAATATTTTC
    ACTATTAGCAATTTTGTTAAGATACATAATGCTCCAAAACATTTTCAAACTGAAAAAATC
    GTAGGTCATATTTCTAGAATGGTACCAACGAAGCGAATTGATTTGCTTATTGAAGTGGCT
    GAGTTAGTCGTAAAAAAAGATAATGCTGTTAAATTTCATATATATGGAGAAGGATCTGTC
    AAAGATAAAATAGCTAAAATGATTGAAGATAAAAATTTAGAAAGAAATGTTTTTCTTAAA
    GGATATACAACAACTCCACAAAAATGCTTGGAAGATTTTAAATTAGTCGTTTCTACATCT
    CAATATGAAGGTCAAGGGTTAAGTATGATAGAAGCAATGATTTCTAAAAGGCCTGTTGTT
    GCCTTTGACATCAAATACGGACCAAGTGATTTTATAGAAGATAATAAAAATGGTTATTTA
    ATAGAAAACCATAATATTAACGACATGGCTGATAAAATACTTCAGCTTGTTAATAATGAT
    GTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACG
    GAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1482

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1043 [new locus tag: SACOL_RS05325 ]
  • symbol: SACOL1043
  • description: glycosyl transferase, group 1 family protein
  • length: 493
  • theoretical pI: 9.34538
  • theoretical MW: 57274.4
  • GRAVY: -0.49858

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein modification and repairaccessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 117)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsN-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 89.2)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 84.2)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 35.2)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 32.7)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 31.9)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidessucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 29.3)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 29)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 27.7)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 25.5)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 24.8)
    sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 19.6)
    Unknown functionEnzymes of unknown specificityglycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 16.6)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03087; HMM-score: 16.4)
    putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 14.8)
    RNA polymerase sigma factor, SigM family (TIGR02950; HMM-score: 12.8)
  • TheSEED:  
    Cell Wall and CapsuleGram-Positive cell wall componentsTeichoic and lipoteichoic acids biosynthesis Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52) 
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 112.3)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 88.5)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 32.5)
    no clan definedDUF4116; Domain of unknown function (DUF4116) (PF13475; HMM-score: 13.9)
    DUF3695; Protein of unknown function (DUF3695) (PF12494; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.168
    • Ymax_pos: 15
    • Cmax: 0.125
    • Cmax_pos: 33
    • Smax: 0.371
    • Smax_pos: 10
    • Smean: 0.277
    • D: 0.211
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYKGNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEIEESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMADKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2]
    quantitative data / protein copy number per cell: 203 [3]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 9.61 h [4]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  4. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Solmaz Sobhanifar, Liam James Worrall, Robert J Gruninger, Gregory A Wasney, Markus Blaukopf, Lars Baumann, Emilie Lameignere, Matthew Solomonson, Eric D Brown, Stephen G Withers, Natalie C J Strynadka
Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid α-glycosyltransferase.
Proc. Natl. Acad. Sci. U.S.A.: 2015, 112(6);E576-85
[PubMed:25624472] [WorldCat.org] [DOI] (I p)