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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1066 [new locus tag: SACOL_RS05435 ]
  • pan locus tag?: SAUPAN003266000
  • symbol: SACOL1066
  • pan gene symbol?: fmtA
  • synonym:
  • product: fmt protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1066 [new locus tag: SACOL_RS05435 ]
  • symbol: SACOL1066
  • product: fmt protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1074466..1075659
  • length: 1194
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAATTTAATAAAGTAAAACTAGTTATACATGCGTGTGTACTATTATTTATCATTATT
    TCTATAGCGTTAATTTTTCATCGATTACAGACGAAGACACATTCTATAGACCCAATACAT
    AAGGAAACAAAATTATCAGACAATGAAAAATATTTAGTGGATCGTAATAAGGAAAAGGTT
    GCGCCGTCTAAACTAAAAGAGGTATATAATAGCAAGGATCCTAAATATAAGAAAATTGAC
    AAGTATTTACAAAGTTCATTATTTAACGGTTCAGTAGCTATATATGAAAATGGCAAATTG
    AAAATGAGTAAAGGTTATGGATATCAAGATTTTGAAAAAGGTATTAAAAACACACCGAAT
    ACGATGTTTTTAATAGGTTCAGCTCAAAAATTTTCAACAGGGTTACTGTTAAAACAGTTA
    GAAGAAGAACATAAAATAAATATCAATGATCCAGTAAGTAAATACCTTCCATGGTTTAAA
    ACATCTAAGCCTATCCCATTGAAAGATTTAATGTTGCATCAAAGTGGATTATATAAATAT
    AAATCCTCAAAAGATTATAAAAATTTAGATCAAGCAGTTAAAGCGATTCAAAAACGTGGT
    ATTGATCCTAAGAAATACAAAAAGCATATGTATAACGATGGGAATTATTTAGTACTTGCG
    AAAGTAATTGAAGAAGTTACAGGTAAATCTTATGCTGAGAATTATTATACAAAAATAGGA
    GATCCTTTAAAACTTCAGCACACAGCATTTTATGATGAACAACCTTTTAAAAAATATCTA
    GCAAAAGGTTATGCTTATAATAGTACAGGACTTTCATTCTTAAGACCTAATATTTTGGAC
    CAATACTATGGTGCAGGTAATTTATATATGACACCAACAGATATGGGTAAATTAATTACT
    CAAATACAACAATATAAATTATTCAGTCCTAAAATAACCAATCCATTATTACATGAGTTT
    GGTACGAAAAAGTATCCAGATGAATATCGATATGGTTTCTATGCTAAGCCAACATTGAAT
    AGACTTAACGGGGGATTCTTTGGACAAGTCTTTACTGTTTACTATAATGATAAGTATGTA
    GTTGTACTTGCATTAAATGTAAAAGGAAACAATGAAGTTCGAATCAAACATATTTATAAT
    GATATTTTAAAACAAAATAAACCTTACAATACGAAGGGTGTTATTGTTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1194

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1066 [new locus tag: SACOL_RS05435 ]
  • symbol: SACOL1066
  • description: fmt protein
  • length: 397
  • theoretical pI: 10.1143
  • theoretical MW: 46067.1
  • GRAVY: -0.561713

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Regulation and Cell signalingRegulation and Cell signaling - no subcategoryCell envelope-associated LytR-CpsA-Psr transcriptional attenuators FmtA protein involved in methicillin resistance 
  • PFAM:
    Beta-lactamase (CL0013) Beta-lactamase; Beta-lactamase (PF00144; HMM-score: 209.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.243
    • Ymax_pos: 36
    • Cmax: 0.148
    • Cmax_pos: 36
    • Smax: 0.669
    • Smax_pos: 5
    • Smean: 0.46
    • D: 0.327
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETKLSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKMSKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFKTSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLVLAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRPNILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFYAKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTKGVIVQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL1066 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]