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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1213 [new locus tag: SACOL_RS06215 ]
  • pan locus tag?: SAUPAN003481000
  • symbol: pyrC
  • pan gene symbol?: pyrC
  • synonym:
  • product: dihydroorotase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1213 [new locus tag: SACOL_RS06215 ]
  • symbol: pyrC
  • product: dihydroorotase
  • replicon: chromosome
  • strand: +
  • coordinates: 1220347..1221621
  • length: 1275
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAATTAATTAAAAACGGTAAAGTATTACAAAATGGCGAATTACAACAAGCAGATATT
    TTAATTGATGGTAAGGTAATTAAACAAATTGCACCTGCAATTGAACCAAGCAATGGTGTT
    GACATCATAGATGCGAAAGGTCACTTTGTGTCACCTGGATTTGTCGATGTTCATGTTCAT
    TTACGTGAACCTGGTGGTGAATATAAAGAGACAATTGAAACTGGTACTAAAGCTGCTGCT
    AGAGGCGGATTTACAACTGTATGTCCAATGCCTAACACAAGACCGGTACCAGATTCTGTA
    GAACATTTTGAAGCTTTACAAAAATTAATCGATGACAATGCTCAAGTACGTGTATTACCT
    TATGCTTCAATTACAACACGTCAATTAGGTAAAGAATTGGTTGATTTCCCAGCACTAGTA
    AAAGAAGGTGCCTTTGCGTTTACAGATGACGGTGTAGGAGTACAAACTGCAAGCATGATG
    TATGAAGGCATGATTGAAGCTGCAAAAGTAAACAAAGCCATCGTAGCACACTGTGAAGAT
    AATTCATTAATCTATGGTGGTGCAATGCATGAAGGGAAACGCAGTAAAGAGTTAGGTATA
    CCAGGTATTCCAAACATTTGTGAATCTGTTCAAATCGCAAGAGATGTACTATTAGCTGAA
    GCAGCAGGTTGTCATTATCATGTATGTCATGTTTCTACTAAAGAAAGTGTTAGAGTCATT
    CGTGACGCTAAACGCGCAGGCATTCATGTTACAGCTGAAGTTACACCACACCATTTATTG
    TTAACAGAAGATGATATTCCTGGTAATAATGCCATTTATAAAATGAATCCACCATTGAGA
    AGTACTGAAGATAGAGAGGCTTTGTTAGAAGGGTTACTAGACGGTACAATTGACTGTATC
    GCAACAGACCATGCACCACATGCACGTGATGAAAAAGCACAACCAATGGAAAAAGCACCA
    TTCGGAATTGTTGGTAGTGAAACAGCATTCCCATTATTATATACGCATTTTGTAAAAAAT
    GGTGATTGGACATTACAACAATTAGTAGATTACTTAACAATTAAACCATGTGAGACATTT
    AATTTAGAATACGGCACATTAAAAGAAAATGGTTATGCAGATTTAACAATCATTGATTTA
    GATAGTGAACAAGAAATTAAAGGAGAAGATTTCTTATCAAAAGCAGATAATACACCATTT
    ATCGGCTATAAAGTTTATGGAAATCCGATCTTAACAATGGTTGAAGGCGAAGTTAAATTT
    GAGGGGGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1275

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1213 [new locus tag: SACOL_RS06215 ]
  • symbol: PyrC
  • description: dihydroorotase
  • length: 424
  • theoretical pI: 4.84608
  • theoretical MW: 46371.4
  • GRAVY: -0.22783

Function[edit source | edit]

  • reaction:
    EC 3.5.2.3?  ExPASy
    Dihydroorotase(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisdihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 471.8)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 245.6)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 201.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisdihydroorotase, homodimeric type (TIGR00856; EC 3.5.2.3; HMM-score: 47)
    Hypothetical proteinsConservedputative amidohydrolase, EF_0837/AHA_3915 family (TIGR03583; HMM-score: 43.1)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesbeta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 33.8)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotidesadenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 28.1)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 27)
    MetabolismEnergy metabolismOtherphosphonate metabolism protein PhnM (TIGR02318; HMM-score: 26.7)
    MetabolismEnergy metabolismAmino acids and aminesimidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 25.9)
    MetabolismCentral intermediary metabolismAmino sugarsN-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 24.7)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 18.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesOtherguanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 18.2)
  • TheSEED:  
    Nucleosides and NucleotidesPyrimidinesDe Novo Pyrimidine Synthesis Dihydroorotase (EC 3.5.2.3) 
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 161.9)
    DHOase; Dihydro-orotase-like (PF12890; HMM-score: 120)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 39.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.123
    • Ymax_pos: 34
    • Cmax: 0.14
    • Cmax_pos: 34
    • Smax: 0.124
    • Smax_pos: 18
    • Smean: 0.1
    • D: 0.114
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIIDAKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEHFEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMMYEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLLAEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMNPPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLYTHFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFLSKADNTPFIGYKVYGNPILTMVEGEVKFEGDK

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 703 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 25.64 h [6]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]