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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1308 [new locus tag: SACOL_RS06675 ]
  • pan locus tag?: SAUPAN003592000
  • symbol: SACOL1308
  • pan gene symbol?:
  • synonym:
  • product: pyruvate ferredoxin oxidoreductase subunit alpha

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1308 [new locus tag: SACOL_RS06675 ]
  • symbol: SACOL1308
  • product: pyruvate ferredoxin oxidoreductase subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 1324576..1326336
  • length: 1761
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAACCACAATTATCGTGGAAAGTTGGCGGTCAACAAGGCGAAGGTATTGAATCAACT
    GGGGAAATCTTCGCTACGGCTATGAATAGAAAAGGATATTATTTATATGGATATAGACAT
    TTTTCAAGTCGTATCAAAGGTGGACATACGAATAATAAAATTAGAGTTTCTACGACGCCT
    GTTCATGCAATTAGTGATGATTTAGATATTTTGATTGCATTTGACCAAGAAACAATTGAT
    GTTAACCATCATGAAATGAGAGAAGACAGTATTATTTTAGCTGATGCCAAGGCTAAACCT
    GTGAAACCAGAAGGATGTCATGCACAGCTTATTGAATTACCTTTTACAGCAACCGCTAAA
    GAATTAGGTACAGCATTAATGAAAAACATGGTTGCAATAGGTGCTACTAGCGCATTGATG
    AATTTGAATACAAATACATTTGAAGAACTTATTACTAATATGTTTTCTAAAAAAGGTGAC
    AAGGTAGTTGAAGTCAATATCCAAGCATTAAACGAAGGTTATCAATTAATGCAATCTCGC
    TTACCTGAAATCTACGGGGACTTTGAATTAGAGTCAACAGATGCACTACCACATCTATAT
    ATGATTGGTAACGATGCCATTGGATTAGGTGCAATTGCTGCAGGTTCACAATTTATGGCG
    GCATATCCTATTACACCTGCGTCTGAAGTTATGGAATATATGATTGCCAATATATCTAAA
    GTAAACGGAGCGGTTATTCAAACAGAAGATGAAATTGCTGCTGTAACTATGGCTATTGGT
    GCAAATTATGGTGGAGTCAGAGCGTTTACGGCTAGTGCTGGTCCAGGTTTATCTTTAATG
    ATGGAAGCAATTGGATTATCTGGTATGACTGAAACGCCATTAGTCATTATTAATACCCAA
    CGAGGTGGACCTTCTACTGGATTACCTACGAAACAAGAACAGTCAGATTTAATGCAAATG
    ATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGAT
    GCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATA
    GTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAAT
    CGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGAT
    AAAGGTTATTTCAAGCGTTATGCGTTAACATCCGATGGTGTTTCTCCTAGACCTATCCCC
    GGTGTTAAAGGAGGTATTCATCATATAACTGGTGTGGAACACAATGAAGAAGGTAAACCT
    AGTGAATCTGCGTCAAATAGACAACAACAAATGGAAAAACGAATGCGTAAAATTGAGCAG
    TTACTAATTGAATCGCCAGTAGAAGCTAACTTACAACATGAGGATGCAGATATTCTTTAT
    ATCGGTTTTATTTCTACAAAAGGTGCAATTCAAGAAGGTAGTAACCGTTTGAATCAACAA
    GGCATAAAAGTTAACACTATACAAATTAGACAATTGCATCCATTCCCAACAAGCGTTATT
    CAAGATGCAGTTAATAAAGCGAAGAAAGTCGTTGTAGTGGAGCACAATTATCAAGGACAA
    TTGGCTAGTATTATAAAAATGAATGTCAATATTCATGATAAGATTGAAAATTATACAAAG
    TATGATGGGACACCTTTCCTACCACATGAAATCGAAGAAAAAGGCAAAATAATTGCTACT
    GAAATAAAGGAGATGGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1761

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1308 [new locus tag: SACOL_RS06675 ]
  • symbol: SACOL1308
  • description: pyruvate ferredoxin oxidoreductase subunit alpha
  • length: 586
  • theoretical pI: 5.06399
  • theoretical MW: 64555.1
  • GRAVY: -0.276621

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    2-oxoacid:acceptor oxidoreductase, alpha subunit (TIGR03710; HMM-score: 712.6)
    indolepyruvate ferredoxin oxidoreductase, alpha subunit (TIGR03336; EC 1.2.7.8; HMM-score: 89.1)
    2-oxoacid:acceptor oxidoreductase, gamma subunit, pyruvate/2-ketoisovalerate family (TIGR02175; HMM-score: 37.1)
    pyruvate:ferredoxin (flavodoxin) oxidoreductase (TIGR02176; EC 1.2.7.1; HMM-score: 25.3)
    indolepyruvate ferredoxin oxidoreductase, beta subunit (TIGR03334; EC 1.2.7.8; HMM-score: 25.2)
    MetabolismAmino acid biosynthesisAromatic amino acid family3-dehydroquinate dehydratase, type I (TIGR01093; EC 4.2.1.10; HMM-score: 24.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 22.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 22.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 22.9)
    MetabolismEnergy metabolismTCA cycleoxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 12.1)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismTCA Cycle 2-oxoglutarate oxidoreductase, alpha subunit (EC 1.2.7.3) 
  • PFAM:
    THDP-binding (CL0254) POR_N; Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg (PF01855; HMM-score: 214.2)
    no clan definedPOR; Pyruvate ferredoxin/flavodoxin oxidoreductase (PF01558; HMM-score: 136.9)
    TKC_like (CL0591) PFOR_II; Pyruvate:ferredoxin oxidoreductase core domain II (PF17147; HMM-score: 40.6)
    THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 30.1)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 24.4)
    TIM_barrel (CL0036) DHquinase_I; Type I 3-dehydroquinase (PF01487; HMM-score: 14.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.131
    • Ymax_pos: 64
    • Cmax: 0.175
    • Cmax_pos: 64
    • Smax: 0.167
    • Smax_pos: 47
    • Smean: 0.085
    • D: 0.113
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSRIKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVKPEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGDKVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQFMAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPGLSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVAPTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQSDIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQMEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQIRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPFLPHEIEEKGKIIATEIKEMV

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 738 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 15.25 h [5]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]