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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1362 [new locus tag: SACOL_RS06930 ]
  • pan locus tag?: SAUPAN003700000
  • symbol: hom
  • pan gene symbol?: hom
  • synonym:
  • product: homoserine dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1362 [new locus tag: SACOL_RS06930 ]
  • symbol: hom
  • product: homoserine dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1367193..1368473
  • length: 1281
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAAAAATTAAATATAGCATTATTAGGATTAGGTACTGTCGGATCTGGTGTTGTTAAA
    ATCATCGAAGAGAACCGACAGCAAATTCAAGATACATTAAATAAAGATATTGTCATAAAG
    CATATTCTTGTTCGAGATAAATCTAAAAAGAGACCGCTAAATATTAGCCAATATCATTTA
    ACTGAAGATGTTAATGAAATTTTAAATGATGATTCATTAGATATTATCGTTGAAGTCATG
    GGAGGAATTGAACCAACTGTAGATTGGTTAAGAACAGCACTTAAAAATAAAAAACATGTT
    ATTACCGCAAATAAAGATTTATTAGCAGTACATCTTAAACTTTTAGAAGATTTAGCAGAA
    GAAAATGGTGTAGCTTTAAAGTTTGAAGCGAGTGTAGCAGGTGGTATTCCGATCGTAAAT
    GCCATAAATAATGGTTTGAATGCGAATAATATTTCAAAATTTATGGGAATTTTAAATGGT
    ACCTCTAATTTTATTTTATCTAAAATGACTAAAGAGCAAACGACATTTGAGGAAGCACTT
    GATGAAGCGAAAAGACTTGGTTTTGCTGAAGCGGATCCAACTGATGATGTAGAAGGGGTA
    GATGCAGCGCGTAAAGTTGTCATTACATCATATTTATCATTTAACCAAGTCATTAAATTA
    AACGACGTTAAACGAAGAGGAATTAGTGGCGTAACTTTAACTGATATTAATGTAGCCGAT
    CAACTGGGGTATAAAATTAAATTGATTGGTAAGGGAATATATGAAAATGGCAAAGTTAAT
    GCATCGGTAGAACCAACGTTAATTGATAAAAAGCATCAATTAGCAGCTGTAGAGGATGAA
    TATAACGCGATTTATGTTATTGGTGATGCCGTTGGTGACACGATGTTTTATGGAAAAGGA
    GCAGGCAGTTTAGCAACAGGTAGTGCCGTTGTCAGTGATTTATTGAATGTAGCATTATTC
    TTTGAATCAGATTTACACACATTGCCACCACATTTTGAATTAAAGACAGATAAAACACGG
    GAAATGATGGATTCAGATGCAGAAATTAATATTAAAGAAAAATCCAATTTCTTTGTAGTA
    GTGAATCATGTCAAAGGTTCAATTGAAAATTTTGAAAATGAGTTAAAGGCAATATTACCA
    TTTCACCGATCATTAAGAGTTGCAAATTACGATAATCAATCATATGCCGCTGTTATAGTT
    GGATTGGAATCATCACCGGAAGAATTAATCACTAAGCATGGATACGAAGTTGACAAAGTA
    TACCCAGTAGAAGGAGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1281

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1362 [new locus tag: SACOL_RS06930 ]
  • symbol: Hom
  • description: homoserine dehydrogenase
  • length: 426
  • theoretical pI: 5.04475
  • theoretical MW: 46873.2
  • GRAVY: -0.126291

Function[edit source | edit]

  • reaction:
    EC 1.1.1.3?  ExPASy
    Homoserine dehydrogenaseL-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H
  • TIGRFAM:
    Signal transductionRegulatory functionsProtein interactionsPhr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 12.4)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineMethionine Biosynthesis Homoserine dehydrogenase (EC 1.1.1.3) 
    Lysine, threonine, methionine, and cysteineThreonine and Homoserine Biosynthesis Homoserine dehydrogenase (EC 1.1.1.3) 
  • PFAM:
    no clan definedHomoserine_dh; Homoserine dehydrogenase (PF00742; HMM-score: 201.5)
    NADP_Rossmann (CL0063) NAD_binding_3; Homoserine dehydrogenase, NAD binding domain (PF03447; HMM-score: 65.9)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 14)
    DXP_reductoisom; 1-deoxy-D-xylulose 5-phosphate reductoisomerase (PF02670; HMM-score: 13.6)
    no clan definedImg2; Mitochondrial large subunit ribosomal protein (Img2) (PF05046; HMM-score: 13.3)
    HAD (CL0137) Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.211
    • Ymax_pos: 22
    • Cmax: 0.13
    • Cmax_pos: 22
    • Smax: 0.471
    • Smax_pos: 2
    • Smean: 0.355
    • D: 0.267
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILVRDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVITANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNGTSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVIKLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAAVEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFELKTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDNQSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 421 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]