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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1403 [new locus tag: SACOL_RS07140 ]
  • pan locus tag?: SAUPAN003762000
  • symbol: trpE
  • pan gene symbol?: trpE
  • synonym:
  • product: anthranilate synthase component I

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1403 [new locus tag: SACOL_RS07140 ]
  • symbol: trpE
  • product: anthranilate synthase component I
  • replicon: chromosome
  • strand: +
  • coordinates: 1414210..1415616
  • length: 1407
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGATATATTTTATAAAAAAATAAAAGCAAATGTAACGCCCGAAGTTTTAGCACAACTT
    CATTCCAAGAAGATCATTTTGGAAAGTACAAATCAACAACAAACTAAAGGTCGCTATTCA
    GTTGTTATTTTTGATATTTATGGCACTTTAACTTTAGATAATGATGTATTATCAGTAAGT
    ACTTTAAAAGAATCGTATCAAATCACTGAAAGACCGTACCATTATTTAACGACTAAAATA
    AATGAAGACTACCATAATATTCAAGATGAGCAACTTAAGTCATTACCATTTATATCTGGA
    TATGTTGGGACGTGTAGCTTTGATTTAGTAAGACATGAATTTCCTAAATTGCAATCAATA
    CAATTAGAAGATCACAAGCAGCACGATGTAAGGTTATATATGGTTGAACAAGTTTATGTA
    TTTGACCATTACAAAGATGAGTTATATATCATCGCGACGAATCAATTTTCAAATTCAACA
    AAATCAGATCTTGAGAATCGAGTTATTAAGTCTATCGAAGACTTAACTAAAATCCAACCA
    TTCATGCCTACACAAGATTTTGATTTTAAAACTAAAGAAATTCAATCAAACATTTCTGAA
    GAAAGATTTATCGAAATGATTCAGTATTTCAAAGAGAAAATAACAGAAGGGGATATGTTC
    CAAGTTGTGCCATCAAGAATTTACAAATATGCGCATCATGCTAGTCAGCATTTAAATCAA
    CTTTCGTTTCAACTGTATCAAAATTTAAAACGACAAAACCCAAGTCCATATATGTATTAT
    CTTAATATCGATCAACCATATATTGTCGGTAGTTCTCCCGAAAGTTTCGTAAGTGTCAAA
    GATCAAATTGTAACAACTAATCCTATTGCAGGTACGATTCAACGTGGTGAGACGACACAA
    ATAGATAATGAGAATATGAAACAACTACTTAATGATCCAAAAGAATGCAGCGAACATCGT
    ATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAA
    ATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTATGCATATCGTAAGTGAAGTC
    ACAGGTAAAATAAATCAAAATTTATCGCCAATGACAGTTATTGCGAATTTATTACCAACA
    GGTACCGTTTCAGGTGCACCAAAATTACGTGCAATTGAAAGAATATATGAACAATATCCA
    CATAAACGGGGCGTTTATAGTGGTGGTGTTGGATACATAAATTGTAATCATAACTTAGAT
    TTTGCATTAGCAATTCGAACGATGATGATAGATGAGCAGTATATCAACGTAGAAGCTGGT
    TGTGGCGTTGTATATGATTCTATTCCTGAAAAAGAACTGAATGAAACGAAATTGAAAGCT
    AAAAGCTTATTGGAGGTGAGCCCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1407

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1403 [new locus tag: SACOL_RS07140 ]
  • symbol: TrpE
  • description: anthranilate synthase component I
  • length: 468
  • theoretical pI: 6.51458
  • theoretical MW: 54110.3
  • GRAVY: -0.45235

Function[edit source | edit]

  • reaction:
    EC 4.1.3.27?  ExPASy
    Anthranilate synthaseChorismate + L-glutamine = anthranilate + pyruvate + L-glutamate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 443.9)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 376.6)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 268.7)
    aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 262.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidaminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 240.7)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 191.7)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 182)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneisochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 179.7)
    salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 149.2)
  • TheSEED:  
    Amino Acids and DerivativesAromatic amino acids and derivativesTryptophan synthesis Anthranilate synthase, aminase component (EC 4.1.3.27) 
  • PFAM:
    no clan definedChorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 287)
    Anth_synt_I_N; Anthranilate synthase component I, N terminal region (PF04715; HMM-score: 97.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.147
    • Ymax_pos: 29
    • Cmax: 0.111
    • Cmax_pos: 38
    • Smax: 0.294
    • Smax_pos: 17
    • Smean: 0.168
    • D: 0.155
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIFDIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYVGTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNSTKSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGDMFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESFVSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVSKIGTSKITKLMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAIERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPEKELNETKLKAKSLLEVSP

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]