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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1498 [new locus tag: SACOL_RS07630 ]
  • pan locus tag?: SAUPAN003918000
  • symbol: SACOL1498
  • pan gene symbol?: bshA
  • synonym:
  • product: glycosyl transferase, group 1 family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1498 [new locus tag: SACOL_RS07630 ]
  • symbol: SACOL1498
  • product: glycosyl transferase, group 1 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1539471..1540625
  • length: 1155
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGCAAGCGAATGAAGATAGGTATAACATGTTACCCGTCCATGGGTGGTTCTGGAATT
    ATTGCCACAGAATTAGGAATTGAATTAGCAGAGCGAGGTCACGAAGTACATTTTATCACT
    TCAAATATCCCGTTTAGAATAAGAAAACCATTACCGAATATGATTTTTCATCAAGTTGAA
    GTGAATCAATATGCAGTATTTCAGTATCCACCGTATGATATTACGTTAAGTACTAAAATC
    GCTGAAGTTATTAAAGAATATGATCTTGATTTGTTGCATATGCACTATGCTGTACCTCAT
    GCAATTTGTGGGATTTTAGCTCGTGAGATGTCAGGTAAAGATATAAAAATTATGACAACG
    CTACACGGTACTGATATTACTGTTTTGGGCTATGATCATTCACTCCAAGGTGCAATTAAA
    TTTGGCATTGAAAAAAGTGATATTGTGACAAGTGTGAGTAAATCTTTAGCACAAGAAACA
    CATGAAATTATTGAAACTAACAAAGAAATTATACCGATTTATAATTTTGTACGTGAAAAT
    GAGTTTCCAACTAAACATAACACAGCATTAAAATCACAATTTGGTATAGCACCAGATGAA
    AAAGTGTTAATACATGTATCGAATTTTAGACAAGTAAAACGCATAGATACTATTATAGAA
    ACATTTGCAAAAGTACGTGAAAAGATACCTAGTAAATTAATTTTACTTGGAGATGGTCCT
    GAATTAGTGCCAATGCGTCAATTGACGAAAGAGTTAAACGTAGAAGAGGATGTTTTATTT
    TTAGGGAAACAAGATTGCGTAAGTGAATTTTACCAATTATCTGACTTAGTATTATTATTA
    AGTGAAAAAGAAAGCTTTGGACTAACTTTACTTGAGGCTATGAAAACAGGCGTCGTTCCA
    ATTGGTTCAAATGCCGGTGGAATTAAAGAGGTTATTAAACATGGTGAAACTGGATTTGTC
    GTAGATGTGGGTGATTGTGATTCAGCGAGTGACTATGCTATCCGATTACTTGAAGATAAG
    GTGTTATACAACAAACTTCAGAAAAACATGCTAGCAGATATTGCTGAACGTTTTGGATCA
    GAACTTATTACAGATCAATATGAGTATTATTATCAAAAAATGCTAAATGAGCATAACAAA
    AGTAAAGGCGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1155

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1498 [new locus tag: SACOL_RS07630 ]
  • symbol: SACOL1498
  • description: glycosyl transferase, group 1 family protein
  • length: 384
  • theoretical pI: 5.75935
  • theoretical MW: 43268.6
  • GRAVY: -0.143229

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 553.1)
    and 13 more
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 93.1)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 78.9)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 75.6)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 61.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 61.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 55.1)
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 41.9)
    Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 38.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 30.1)
    colanic acid biosynthesis glycosyltransferase WcaI (TIGR04007; EC 2.4.-.-; HMM-score: 29.6)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 26.9)
    sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 25.2)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 24)
  • TheSEED  :
    • Glycosyl transferase, group 1 family protein
    Cell Wall and Capsule Capsular and extracellular polysacchrides Exopolysaccharide Biosynthesis  Glycosyl transferase, group 1 family protein
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 128.5)
    and 8 more
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 84.6)
    Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 72)
    Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 47.9)
    Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 30.8)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 24.1)
    Glyco_transf_5; Starch synthase catalytic domain (PF08323; HMM-score: 22.9)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 15)
    P-loop_NTPase (CL0023) ArgK; ArgK protein (PF03308; HMM-score: 12)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.058167
    • TAT(Tat/SPI): 0.001858
    • LIPO(Sec/SPII): 0.013738
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGKRMKIGITCYPSMGGSGIIATELGIELAERGHEVHFITSNIPFRIRKPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGILAREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEIIETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETFAKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLLSEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLEDKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 111 [5]
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]