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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1519 [new locus tag: SACOL_RS07745 ]
  • pan locus tag?: SAUPAN003943000
  • symbol: ansA
  • pan gene symbol?: ansA
  • synonym:
  • product: L-asparaginase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1519 [new locus tag: SACOL_RS07745 ]
  • symbol: ansA
  • product: L-asparaginase
  • replicon: chromosome
  • strand: +
  • coordinates: 1557882..1558850
  • length: 969
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAACATCTACTTGTTATTCATACTGGTGGCACCATTAGTATGTCACAAGACCAATCT
    AATAAAGTAGTAACAAATGATATCAACCCTATTTCAATGCATCAAGATGTCATAAATCAA
    TATGCACAAATAGATGAATTAAATCCTTTTAATGTACCATCACCTCATATGACAATCCAA
    CATGTTAAACAATTAAAGGATATTATTTTAGAAGCAGTAACAAATAAATATTATGATGGT
    TTCGTTATCACGCATGGTACCGATACGTTAGAAGAAACTGCCTTTTTACTTGATTTAATA
    TTAGGTATCGAGCAACCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGT
    TCTGACGGATTATATAATTATATTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGCCCGT
    CATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACGGCGCGTAATGTTACCAAA
    ACACATACGTCTAATACAAACACATTTCAAAGTCCAAATCATGGTCCGCTAGGTGTATTG
    ACAAAGGATCGTGTGCAATTCCATCATATGCCATATCGCCAACAAGCATTGGAAAATGTC
    AATGACAAACTAAATGTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTT
    AGTTTTTATAGTCGAGAAGGTATCGATGGTATGGTTATTGAAGCGTTAGGACAAGGCAAC
    ATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTG
    CTAGTTTCACGTTCCTTTAATGGTATTGTGAGTCCAACTTACGCATACGATGGTGGTGGT
    TACCAACTCGCACAACAAGGTTTTATTTTTTCTAACGGCTTGAATGGTCCAAAAGCAAGA
    TTAAAATTATTAGTCGCGTTAAGCAACAATTTAGATAAAGCTGAAATCAAATCATATTTT
    GAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1519 [new locus tag: SACOL_RS07745 ]
  • symbol: AnsA
  • description: L-asparaginase
  • length: 322
  • theoretical pI: 6.3723
  • theoretical MW: 35578.3
  • GRAVY: -0.150932

Function[edit | edit source]

  • reaction:
    EC 3.5.1.1?  ExPASy
    Asparaginase L-asparagine + H2O = L-aspartate + NH3
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines L-asparaginase, type II (TIGR00520; EC 3.5.1.1; HMM-score: 234.8)
    and 2 more
    L-asparaginase, type I (TIGR00519; EC 3.5.1.1; HMM-score: 176.5)
    Genetic information processing Protein synthesis tRNA aminoacylation glutamyl-tRNA(Gln) amidotransferase, subunit D (TIGR02153; EC 6.3.5.-; HMM-score: 131.8)
  • TheSEED  :
    • L-asparaginase (EC 3.5.1.1)
    Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis  L-asparaginase (EC 3.5.1.1)
  • PFAM:
    no clan defined Asparaginase; Asparaginase, N-terminal (PF00710; HMM-score: 201)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.037167
    • TAT(Tat/SPI): 0.000591
    • LIPO(Sec/SPII): 0.02003
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTKTHTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]