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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1595 [new locus tag: SACOL_RS08125 ]
  • pan locus tag?: SAUPAN004114000
  • symbol: gcvT
  • pan gene symbol?: gcvT
  • synonym:
  • product: glycine cleavage system aminomethyltransferase T

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1595 [new locus tag: SACOL_RS08125 ]
  • symbol: gcvT
  • product: glycine cleavage system aminomethyltransferase T
  • replicon: chromosome
  • strand: -
  • coordinates: 1628778..1629869
  • length: 1092
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTCAAGTGATTTAAAACAAACACCTTTATATCAAAATTATGTTGATAGAGGTGCAAAA
    ATTGTGGAATTCGGAGGATGGGCGATGCCTGTTCAATTTTCAAGTATTAAAGAGGAGCAT
    AATGCTGTTCGATACGAAATTGGCCTGTTTGATGTTAGTCATATGGGTGAAATTGAAGTA
    ACAGGTAAAGATGCTAGTCAGTTTGTGCAATATTTATTATCAAATGATACTGATAATTTA
    ACTACTTCAAAAGCATTATATACTGCTTTATGTAATGAAGAAGGCGGTATTATTGATGAT
    TTAGTAATATATAAATTAGCTGACGACAATTATTTATTAGTTGTTAATGCTGCTAATACT
    GAAAAAGATTTTAATTGGATTTTAAAACACAAAGAGAAATTTGATGTTGAAGTACAAAAT
    GTATCAAACCAATATGGTCAATTAGCAATACAAGGACCAAAAGCTAGAGATTTAATTAAT
    CAATTAGTTGATGAAGATGTAACTGAAATGAAAATGTTTGAATTTAAACAGGGTGTCAAA
    TTATTTGGAGCAAACGTCATTTTATCTCAGTCAGGTTACACAGGTGAAGATGGGTTTGAA
    ATTTATTGTAATATCGATGATACTGAAAAAATTTGGGATGGTTTATTAGAGTATAATGTT
    ATGCCATGTGGGTTAGGCGCTCGTGATACATTAAGATTAGAGGCTGGATTGCCATTACAT
    GGACAAGATTTAACTGAATCAATTACACCATATGAAGGTGGTATCGCTTTTGCAAGTAAA
    CCATTAATTGATGCTGATTTTATTGGTAAATCTGTATTAAAAGATCAAAAAGAAAATGGT
    GCACCAAGAAGAACAGTGGGATTAGAATTACTTGAAAAAGGAATTGCAAGAACTGGTTAT
    GAAGTTATGGATTTAGATGGAAATATTATTGGAGAAGTAACTTCAGGAACACAGTCTCCA
    TCATCAGGAAAATCAATTGCACTTGCAATGATAAAAAGAGATGAGTTTGAAATGGGTAGA
    GAGTTGCTTGTTCAAGTTCGTAAGCGTCAATTAAAAGCGAAAATTGTTAAGAAAAATCAA
    ATTGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1595 [new locus tag: SACOL_RS08125 ]
  • symbol: GcvT
  • description: glycine cleavage system aminomethyltransferase T
  • length: 363
  • theoretical pI: 4.54011
  • theoretical MW: 40457.6
  • GRAVY: -0.331956

Function[edit source | edit]

  • reaction:
    EC 2.1.2.10?  ExPASy
    Aminomethyltransferase[Protein]-S8-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesglycine cleavage system T protein (TIGR00528; EC 2.1.2.10; HMM-score: 442)
    MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 187.7)
    MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, gamma subunit family (TIGR01375; EC 1.5.3.1; HMM-score: 22)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10) 
    Alanine, serine, and glycineGlycine cleavage system Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10) 
  • PFAM:
    FBD (CL0289) GCV_T; Aminomethyltransferase folate-binding domain (PF01571; HMM-score: 318.2)
    no clan definedGCV_T_C; Glycine cleavage T-protein C-terminal barrel domain (PF08669; HMM-score: 86.6)
    FBD (CL0289) SoxG; Sarcosine oxidase, gamma subunit family (PF04268; HMM-score: 24.3)
    no clan definedDUF3659; Protein of unknown function (DUF3659) (PF12396; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 37
    • Cmax: 0.13
    • Cmax_pos: 37
    • Smax: 0.143
    • Smax_pos: 33
    • Smean: 0.1
    • D: 0.11
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVRYEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLVIYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLINQLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEYNVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQKENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRDEFEMGRELLVQVRKRQLKAKIVKKNQIDK

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 201 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]