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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1775 [new locus tag: SACOL_RS09085 ]
  • pan locus tag?: SAUPAN004385000
  • symbol: SACOL1775
  • pan gene symbol?: ptaA
  • synonym:
  • product: PTS system, IIBC components

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1775 [new locus tag: SACOL_RS09085 ]
  • symbol: SACOL1775
  • product: PTS system, IIBC components
  • replicon: chromosome
  • strand: -
  • coordinates: 1814875..1816341
  • length: 1467
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTATAAATTTTTCCAAAATTTAGGGCGTTCGTTAATGTTACCAGTTGCCATTTTACCG
    GCTGCAGCAATTATTGCCGGAATTGGTAATACGTTAAATGCACTTCATGCGACGCCGAAA
    ATCGCAATGTTCTTTACTACGGTAGGTACTACGATTTTAGAGCAATTAGGTATTTTATTT
    GCAATTGGTGTAGCTATAGGAATGGCAAAGAAAAATGATGGTGCAGTCGCGCTTGCAGCT
    ACGTTAGGATATTTTTTAGTTACAGTTGTATTATCACCAATGAAATTAGCGCCTTTATTA
    GGAATGAAAGCTTCGGAAATAAATTCAGCTTTTGAAAAGATGAATAATGGCAATGTCTTT
    GTTGGTATTGTAATAGGTTTAATAGCAGCTTATGCATACAATAAGTTTAGTGAAACAGAA
    TTACCATTAGCATTATCATTTTTTAGTGGTAAACGTCTAGTTCCAATTATGACTGCATTT
    TACTGTACATTTTTAGTTGTCATATTGTTATTCTTATGGCCACTACTTTATTCATGGATT
    GTAAAATTTGGTGAATCTATAGTCGGTTTAGGATCGTTTGGAGCTTTCATATATGGTGTT
    GCCAATAGATTATTAATTCCTACAGGATTACATCATGCACTGAATAGTGTGTTCTGGTTT
    GATACGATTGGTATTAATGATATTGGTAAATTCCAAAGTGGCAAAGATGCTATAAAAGGT
    ATTACAGGACGCTATCAAGCGGGATTCTTCCCAATCATGATGTTCGGTATTCCAGCGGCT
    GCGTTAGCAATGTATCATACAGCTAAAACTACTCAGAAAAAACAAGTTTACGGTTGGTTC
    CTAGCGAGTTCAGTTGCAGCATTTTTCGTTGGTGTTACAGAGCCGATTGAGTTTGCATTT
    ATGTTTGTTGCGCCAATCTTATATGTTGTTCATGCGTTATTAACAGGATTATCTTTATTT
    ATCGCAGCTACATTCCATTGGACAGCTGGATTTTCATTTAGTGCAGGTTTGATAGATTAT
    GTGTTATCACTAATTAATCCAGTATCAAATCATCCATTGATGTTGCTTGTTCAAGGTGTT
    GTATTTTTCATCTTATACTATGTGATTTTCCGTGTAGTAATCCAAGTATTTAACTTGAAT
    ACGATTGGTAGAGGTGAAAATGAATTAGTTGACCCAACAGTTGTAAAAGATAATATTGCT
    CCTGGTGAAAATGATATTAAACAAAGTAAATATCATCAACATGCTATACAAATATTAGAA
    GGTTTAGGCGGTCAAGAGAATATTGTTAATTTAACCAATTGTGCAACAAGGTTGCGTCTA
    GAGTTAAAAGACACATCTATTATTGATAAACAGAAGATTAAAAACGCCGGTGCAGTTGGT
    GTTACTGTTAATGGCAAACATTCAACTCAAGTTATTGTCGGTACACACGTACAGCAGGTT
    GCTGATGAAATTGAAAAACATTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1467

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1775 [new locus tag: SACOL_RS09085 ]
  • symbol: SACOL1775
  • description: PTS system, IIBC components
  • length: 488
  • theoretical pI: 9.38912
  • theoretical MW: 53086.2
  • GRAVY: 0.625205

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 714.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 381.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 292.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 231.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 196.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 176.1)
    Signal transductionSignal transductionPTSPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 176.1)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 58)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 56.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 46.2)
    Signal transductionSignal transductionPTSPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 46.2)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 44.7)
  • TheSEED:  
    Chitin and N-acetylglucosamine utilization PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component 
    Sialic Acid Metabolism PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component 
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 233.1)
    no clan definedPTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 57.2)
    Oxa1 (CL0376) 60KD_IMP; 60Kd inner membrane protein (PF02096; HMM-score: 10.8)
    no clan definedSH; Viral small hydrophobic protein (PF01445; HMM-score: 8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.283
    • Ymax_pos: 24
    • Cmax: 0.248
    • Cmax_pos: 38
    • Smax: 0.634
    • Smax_pos: 13
    • Smean: 0.545
    • D: 0.385
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMFFTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGMKASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAFYCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVFWFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQVYGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFSAGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDPTVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDKQKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]