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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1832 [new locus tag: SACOL_RS09395 ]
  • pan locus tag?: SAUPAN004482000
  • symbol: crcB
  • pan gene symbol?: crcB1
  • synonym:
  • product: camphor resistance protein CrcB

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1832 [new locus tag: SACOL_RS09395 ]
  • symbol: crcB
  • product: camphor resistance protein CrcB
  • replicon: chromosome
  • strand: +
  • coordinates: 1886613..1887056
  • length: 444
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGCATCGACAATTTTTGTCGTCGCGTTGCCAAAACCTCTTTTTTAAATTCAAACTACTT
    CTTTTCGAGGTGAACCAAATGCAATATGTATATATTTTTATCGGTGGTGCTTTAGGCGCT
    TTATTACGTTACCTCATTTCTTTTCTGAATACTGACGGAGGTTTTCCAATCGGAACACTG
    ATAGCCAATTTGACTGGTGCCTTTGTAATGGGATTGCTAACAGCCTTAACAATTGCATTT
    TTTTCAAACCATCCGACCCTAAAAAAAGCTATTACGACTGGTTTTCTTGGTGCTTTAACG
    ACTTTTTCAACATTTCAATTAGAATTAATACATATGTTTGATCATCAACAATTTATAACT
    TTACTACTATATGCTGTAACAAGTTATGTCTTTGGTATTTTGTTATGTTACGTCGGTATA
    AAACTAGGTGGTGGTTTATCATGA
    60
    120
    180
    240
    300
    360
    420
    444

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1832 [new locus tag: SACOL_RS09395 ]
  • symbol: CrcB
  • description: camphor resistance protein CrcB
  • length: 147
  • theoretical pI: 9.45493
  • theoretical MW: 16352.3
  • GRAVY: 0.817687

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionGeneralprotein CrcB (TIGR00494; HMM-score: 84.1)
  • TheSEED:  
    Protein crcB homolog 1 
  • PFAM:
    DMT (CL0184) CRCB; CrcB-like protein, Camphor Resistance (CrcB) (PF02537; HMM-score: 91.3)
    UMP_1 (CL0470) ThrE_2; Threonine/Serine exporter, ThrE (PF12821; HMM-score: 17.2)
    Holin-III (CL0564) Phage_holin_3_3; LydA holin phage, holin superfamily III (PF16083; HMM-score: 11.3)
    Phage_holin_3_1; Phage holin family (Lysis protein S) (PF05106; HMM-score: 11.3)
    no clan definedDUF2070; Predicted membrane protein (DUF2070) (PF09843; HMM-score: 7.1)
    CbtA; Probable cobalt transporter subunit (CbtA) (PF09490; HMM-score: 7.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.187
    • Ymax_pos: 13
    • Cmax: 0.112
    • Cmax_pos: 40
    • Smax: 0.461
    • Smax_pos: 9
    • Smean: 0.353
    • D: 0.251
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MHRQFLSSRCQNLFFKFKLLLFEVNQMQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVMGLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTSYVFGILLCYVGIKLGGGLS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]