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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1945 [new locus tag: SACOL_RS10170 ]
  • pan locus tag?: SAUPAN004902000
  • symbol: SACOL1945
  • pan gene symbol?: vraU
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1945 [new locus tag: SACOL_RS10170 ]
  • symbol: SACOL1945
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2007316..2007702
  • length: 387
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAACTATGTTGAACGTTATATTGAACAGTTTTTGAGAGCAACAGTAAGAAATAATATC
    AAGCACTACCTTTTAATGCTAGATGAAAAAATGAAAAATTTAGATGATTATATGCGTTAT
    TTAATTACTAAAAAAGAACAACTTAGCAAGTTAATTGACAGTCTAATGCTAACATTAGAA
    AATAAATATATTGATATTGCTGAAGCATTTCAAATTCAATGTGCAAGAGAAATCAATAAT
    CAAGAAATTGAAAATATTAAATCAGAGTTGAATAAAGTTGAAGCATATTATGCACAAATT
    GAAACTCAAATTCAACAAACTTCAACTGAAAAAATAGCAACAGAAAAAACATCGTATCTA
    ATAAATTATATGAACGCTGTGGCATAG
    60
    120
    180
    240
    300
    360
    387

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1945 [new locus tag: SACOL_RS10170 ]
  • symbol: SACOL1945
  • description: hypothetical protein
  • length: 128
  • theoretical pI: 4.98573
  • theoretical MW: 15289.5
  • GRAVY: -0.490625

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    two transmembrane protein (TIGR04527; HMM-score: 15.6)
    flagellar export protein FliJ (TIGR02473; HMM-score: 12.9)
    Genetic information processingProtein fateProtein folding and stabilizationprefoldin, alpha subunit (TIGR00293; HMM-score: 11.9)
  • TheSEED:  
    FIG01108212: hypothetical protein 
  • PFAM:
    no clan definedDUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 15.7)
    Fusion_gly (CL0595) Baculo_F; Baculovirus F protein (PF12259; HMM-score: 14.4)
    no clan definedDUF1978; Domain of unknown function (DUF1978) (PF09321; HMM-score: 14.2)
    DAPDH_C; Diaminopimelic acid dehydrogenase C-terminal domain (PF16654; HMM-score: 13.9)
    DUF4795; Domain of unknown function (DUF4795) (PF16043; HMM-score: 13.7)
    FliD_C; Flagellar hook-associated protein 2 C-terminus (PF07195; HMM-score: 12.3)
    tRNA_bind_arm (CL0298) Seryl_tRNA_N; Seryl-tRNA synthetase N-terminal domain (PF02403; HMM-score: 12.2)
    no clan definedApoO; Apolipoprotein O (PF09769; HMM-score: 11.5)
    Mer2; Mer2 (PF09074; HMM-score: 10.8)
    SKA2; Spindle and kinetochore-associated protein 2 (PF16740; HMM-score: 10.3)
    GAG-polyprotein (CL0523) Retrotrans_gag; Retrotransposon gag protein (PF03732; HMM-score: 9.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.104
    • Ymax_pos: 11
    • Cmax: 0.104
    • Cmax_pos: 34
    • Smax: 0.15
    • Smax_pos: 2
    • Smean: 0.106
    • D: 0.105
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITKKEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIETQIQQTSTEKIATEKTSYLINYMNAVA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Susan Boyle-Vavra, Shouhui Yin, Dae Sun Jo, Christopher P Montgomery, Robert S Daum
VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus.
Antimicrob. Agents Chemother.: 2013, 57(1);83-95
[PubMed:23070169] [WorldCat.org] [DOI] (I p)