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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2000 [new locus tag: SACOL_RS10450 ]
  • pan locus tag?: SAUPAN005004000
  • symbol: SACOL2000
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2000 [new locus tag: SACOL_RS10450 ]
  • symbol: SACOL2000
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2059672..2060958
  • length: 1287
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATCCTTTAGCCCAAAGCTTAAATGAACAACTTCAACAGTCAAATGCAACTGCCTTT
    ACAATGTTATCTGACTTAGGTCAAAATATGTTTTATCCAAAAGGCATTTTATCTCAATCT
    GCTGAAGCAAAGAGTACAACATATAATGCAACTATAGGTATGGCGACAAACAAAGACGGA
    AAAATGTTTGCATCATCTTTAGATGCAATGTTTAATGATTTAACTCCAGATGAAATATTC
    CCTTATGTGCCACCACAAGGCATCGAAGAATTACGTGATTTATGGCAACAAAAAATGTTG
    CGTGACAATCCAGAGCTATCAATCGACAACATGTCACTACCAATTGTTACGAATGCATTA
    ACACATGGTTTATCTTTAGTTGGCGATTTATTTGTAAATCAAGGTGACACTATCTTGTTA
    CCAGAGCATAATTGGGGTAATTACAAACTTGTTTTCAATACGAGAAATGGTGCAAACCTT
    CAAACATATCCTATCTTTGATAAAGACGGGCATTATACTACTGATTCACTTGTAGAAGCT
    TTACAATCATACAATAAAGATAAAGTCATTATGATTTTAAATTATCCTAATAATCCGACA
    GGTTACACACCTACGCATAAAGAAGTGACTACCATCGTCGATGCAATTAAAGCATTAGCT
    GATAAAGGTACAAAAGTTATAGCTGTTGTGGATGACGCATACTATGGTTTATTCTATGAA
    GATGTGTATACTCAATCATTATTTACTGCATTATCTAATTTAAATTCAAATGCAATATTA
    CCTGTTCGTTTAGATGGTGCAACAAAAGAATTTTTCGCATGGGGATTCCGTGTTGGGTTT
    ATGACATTTGGAACGTCTGATCAAACAACTAAAGAAGTATTAGAAGCCAAAGTAAAAGGT
    CTTATACGAAGTAACATTTCTAGTGGACCTCTTCCAACACAAAGCGCTGTTAAGCATGTA
    TTAAAAAATAATAAACAATTCGATAAAGAAATCGAACAAAATATTCAAACATTAAAAGAA
    CGCTATGAAGTCACTAAGGAAGTTGTCTATGCTGATCAATATCATTCACATTGGCAAGCC
    TATGACTTTAACTCTGGATACTTTATGGCAATAAAAGTTCATGATGTTGACCCTGAAGAA
    CTTCGTAAACATTTAATTGAAAAATATTCAATTGGTGTTATTGCACTTAATGCAACAGAT
    ATTCGTATTGCGTTTAGCTGTGTAGAAAAAGAAGATATCCCACATGTATTTGATTCAATT
    GCTAAAGCGATTGATGACTTAAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1287

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2000 [new locus tag: SACOL_RS10450 ]
  • symbol: SACOL2000
  • description: hypothetical protein
  • length: 428
  • theoretical pI: 4.88204
  • theoretical MW: 48147.1
  • GRAVY: -0.310748

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 62.7)
    MetabolismEnergy metabolismAmino acids and aminestyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 61.6)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 56.1)
    MetabolismAmino acid biosynthesisHistidine familyhistidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 43.3)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 35.2)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 29.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminthreonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 26.2)
    Cellular processesCellular processesBiosynthesis of natural productscapreomycidine synthase (TIGR03947; HMM-score: 25.7)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 24.7)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 19.6)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 13.5)
    [FeFe] hydrogenase, group B1/B3 (TIGR04105; EC 1.12.-.-; HMM-score: 10.5)
  • TheSEED:  
    Amino Acids and DerivativesGlutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine, Glutamate, Aspartate and Asparagine Biosynthesis Aspartate aminotransferase (EC 2.6.1.1) 
    Lysine, threonine, methionine, and cysteineThreonine and Homoserine Biosynthesis Aspartate aminotransferase (EC 2.6.1.1) 
    Proline and 4-hydroxyprolineProline, 4-hydroxyproline uptake and utilization Aspartate aminotransferase (EC 2.6.1.1) 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 125.3)
    Thioredoxin (CL0172) AhpC-TSA; AhpC/TSA family (PF00578; HMM-score: 16)
    no clan definedSpore_II_R; Stage II sporulation protein R (spore_II_R) (PF09551; HMM-score: 14.2)
    4HB_MCP (CL0457) 4HB_MCP_1; Four helix bundle sensory module for signal transduction (PF12729; HMM-score: 13.6)
    no clan definedNEMO; NF-kappa-B essential modulator NEMO (PF11577; HMM-score: 10.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.223
    • Ymax_pos: 20
    • Cmax: 0.2
    • Cmax_pos: 20
    • Smax: 0.336
    • Smax_pos: 12
    • Smean: 0.231
    • D: 0.226
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAKSTTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYVPPQGIEELRDLWQQKMLRDNPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANLQTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKALADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGFRVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQNIQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIGVIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 464 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL2000 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: LexA
    LexA (TF) important in SOS responseRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]