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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2176 [new locus tag: SACOL_RS11450 ]
  • pan locus tag?: SAUPAN005581000
  • symbol: opuD2
  • pan gene symbol?: opuD2
  • synonym:
  • product: BCCT family osmoprotectant transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2176 [new locus tag: SACOL_RS11450 ]
  • symbol: opuD2
  • product: BCCT family osmoprotectant transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2256981..2258543
  • length: 1563
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAAAAATAAATCAGTAAGTCTAGTGTTTTGGGTATCTTTAGTGATATGTACGATATTT
    GTTGCTTTTGGAGCAATATTTCCGAAACAGTTAGAAAAACTCACGCAAAACATTACTACA
    TTTATTGCGCTACATTTTTCTTGGTATTATTTATTGCTCGTTTTAGTTATTTTATTTGTC
    TGTGTGTATATTTTATTTTCGAGATACGCAAACATTACATTAGGTGAAGAAGGCGAAGAT
    CCTGAATTCTCATTGCCGTCTTGGTTTGCTATGTTATTTAGTGCTGGTATGGGAATAGGA
    CTTGTGTTCTGGACAACGGCAGAACCAATCAGTCATGCCTTCAAATTAACGCCTATTCAT
    AAAGCTGGCACGCAATCTGCAATTAATGATGCAATGCAGTTTTCATTTTTCCATTGGGGG
    ATTCATGCATGGGCAGTCTATGGTATTGTCGCGCTAGTGTTTGCATATTTTAGCTTTCAT
    AAAGGATATCCTGGCTTAGTCAGCGCTACATTGACGCCTTTATTAGGTGAAAAGGCAATG
    CGTGGACCTTTAGGTGGTGCCATTGATGTATTGGCAGTCATAGCTACAGTAACAGGCGTT
    GCTGCAACATTAGGTTTCGGTGCATTGCAAATAAACGAAGGTTTACATTTTTTATTTAAT
    GTACCGAGTAACTTCACGATGCAAGTAATCTTGATTGTCATTGCAACCATATTATTTACT
    TGGTCAGCATGGTCAGGTATCGATAAAGGTATCAAAACGTTGAGTAATATCAACATGTTA
    CTTGCTTTTGTAGTACTTATTGGTCTTTTTATTGTTGGGCCAACGCTATACATTTTAAAT
    ACATTTACGAATGGTCTAGGTAATTATATTGCAAATTTCTTTAGTATGAGTTTACGCATT
    CCGACAGGTGGTCAAAAATTCCAATGGTTACAAAATTGGACAATCTTTTACTGGGCATGG
    TGGATATCTTGGGCACCATTTGTAGGTATATTTATTGCACGTGTGTCAAAAGGACGTACG
    ATTAAAGAATTTATTCTAGGTGTACTTTTTGTCCCGGCGTTAGTTTGCTTTATATTCTTT
    GCGGTATTCGGCGCGTCAGCAATTTACTTACAAGATAATCATATTGCCGATATTGCTAAA
    GCTGCGACAGAAACAGCAACTTTTGCGACGCTACAACATTATCCGCTAGGTTTTGTATTA
    AGCTTAATTACTTTACTTGTTATTATGATTTTCTTTGTTACATCAGCGGATTCGGCAACT
    TATGTTTTAGGTATGTTGAGTTCAAGTGGTGATATTAATCCGAAGTCATTTGTCAAAGTG
    AGTTGGGGTATCATTATGGCATTATTTGCAATCATCATGATTTACACAGGTGGTACTCAA
    GCAATTCAAAATTTATTAATCATTGCTGCGTTACCATTTTCCGTGGTCATTATAGCGATG
    ATATGGTCGCTTATAAAATCTTTGAGTGAGGAGAAACCGAGAAATAGTAATAAGGTTTTA
    ATTAAACATCGTGACCCAGATATTTTAGAATATCGTTCTTCAAAACATATTGACGAAAAT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1563

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2176 [new locus tag: SACOL_RS11450 ]
  • symbol: OpuD2
  • description: BCCT family osmoprotectant transporter
  • length: 520
  • theoretical pI: 8.91716
  • theoretical MW: 57635.7
  • GRAVY: 0.788654

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsOthertransporter, betaine/carnitine/choline transporter (BCCT) family (TIGR00842; HMM-score: 528.4)
  • TheSEED:  
    Stress ResponseOsmotic stressCholine and Betaine Uptake and Betaine Biosynthesis Glycine betaine transporter OpuD 
  • PFAM:
    APC (CL0062) BCCT; BCCT, betaine/carnitine/choline family transporter (PF02028; HMM-score: 625.6)
    M6PR (CL0226) ATG27; Autophagy-related protein 27 (PF09451; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.317
    • Ymax_pos: 25
    • Cmax: 0.22
    • Cmax_pos: 25
    • Smax: 0.57
    • Smax_pos: 12
    • Smean: 0.473
    • D: 0.378
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFHKGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: RpoF (SigB*) [3] [4] other strains
    RpoF  (SigB*) (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int. J. Med. Microbiol.: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)
  4. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]