From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2361 [new locus tag: SACOL_RS12400 ]
  • pan locus tag?: SAUPAN005873000
  • symbol: SACOL2361
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2361 [new locus tag: SACOL_RS12400 ]
  • symbol: SACOL2361
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2419966..2420421
  • length: 456
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAAAATTTGAAAAACTCATTATTTATTAGCTTAATCATTGGTTTGTCCCTTTCACTG
    TTCTTTAGTATGTTATTTGCAGACGGCAAATATTATCCACTGAACCCACAATCAACTATT
    GGCATATTGTATTATACGCATTTCACAGAAACTACCGTAATGTTGATTTCTATCATTTTA
    TGGTTACTCATAGGCGTCGTCTTTTTCCTTGGAGATTTTATCTTTAAATACACAGATTGG
    AGCATTACTAAAGCAACTATAATGCATTTCATAACAACGTATGTCGGATTCTTGCCTTTA
    GCAATGCTTGCCGGTTGGTTTCCACTAACAGTGCATTATCTAATCATATTCACAATTATC
    TTCATTGTGGTTTATGTATTAATTTGGATAATTCAATTCTTTAAAAACAAGAACTACGTA
    GACACTATTAACAAGCAACTGAAACAATTAAAATGA
    60
    120
    180
    240
    300
    360
    420
    456

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL2361 [new locus tag: SACOL_RS12400 ]
  • symbol: SACOL2361
  • description: hypothetical protein
  • length: 151
  • theoretical pI: 9.82185
  • theoretical MW: 17676.2
  • GRAVY: 0.93245

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis type VI secretion protein IcmF (TIGR03348; HMM-score: 9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein D (TIGR02214; HMM-score: 7.3)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein D (TIGR02214; HMM-score: 7.3)
  • TheSEED  :
    • FIG01108202: hypothetical protein
  • PFAM:
    no clan defined DUF3021; Protein of unknown function (DUF3021) (PF11457; HMM-score: 119.1)
    and 4 more
    DUF4271; Domain of unknown function (DUF4271) (PF14093; HMM-score: 16.6)
    DUF4381; Domain of unknown function (DUF4381) (PF14316; HMM-score: 13.1)
    AsmA-like (CL0401) AsmA; AsmA family (PF05170; HMM-score: 10.8)
    no clan defined Holin_SPP1; SPP1 phage holin (PF04688; HMM-score: 10.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.149266
    • TAT(Tat/SPI): 0.002029
    • LIPO(Sec/SPII): 0.14288
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILYYTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAMLAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]