From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2499 [new locus tag: SACOL_RS13095 ]
  • pan locus tag?: SAUPAN006083000
  • symbol: SACOL2499
  • pan gene symbol?:
  • synonym:
  • product: helicase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2499 [new locus tag: SACOL_RS13095 ]
  • symbol: SACOL2499
  • product: helicase
  • replicon: chromosome
  • strand: -
  • coordinates: 2551139..2554000
  • length: 2862
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGTAGATTACTAAATGATTTCAATCAATCTTTACATAAAGGGTTTATAGACAAACAT
    ATTTCTCATAAAGGGAATTATACACCGAAGCTTTTAGTAAATAATAAAAATGAAAAAGTT
    CTTTCAACAATTATAGATGAACTTCAAAAATGTGAAACGTTTTATTTTTCTGTGGCTTTT
    ATAACAGAAAGCGGTTTAGCCAGCTTAAAGGCTCAACTATTAGATTTAAGCAATAAGGGT
    GTTAAAGGGAAAATATTAACATCTAATTACTTAGGGTTTAATAGTCCTAAAATGTATGGA
    GAATTACTTAAATTAAAAAATGTAGAGGTAAGATTAACTGATATTGCTGGATTCCATGCC
    AAAGGATATATTTTTGAGCATAAGGATTATAGTTCTATGGTAATAGGAAGCTCTAATTTA
    ACATCTAATGCGTTAAAGGTTAATTATGAGCATAATGTTTTACTGTCTACTATGAAAAAT
    GGCGATTTAGTTGATAGTGTAAAAAGTGAATTTGACTTGTTATGGCAAAAGAGTACCCCA
    CTGACTGAGCAATGGATTAATTCATATAAAGAATCATTTGAGTACCGTTCCTTAGAAAAA
    TTAGCGGAGGTTGAACAAACTCAAATGCTGCTAGCTGATAAGGTGAAAAAATCAGTGGAA
    ATTGTACCCAATTTAATGCAAGCAGAAGCATTAAGGTCATTAAAAGCTATTAGGGATAAA
    GCCAAAGATAAGGCGTTAATAATATCTGCAACTGGTACAGGTAAAACGATTTTATGTGCA
    TTAGATGTTAGAGAAGTAAACCCTAATAAATTTTTATTTATTGTTCATAATGAGGGGATT
    TTAAATAGAGCTAAGGAAGAATTTAAAAAAGTATTACCTATAAAAAATGATAGTGATTTT
    GGATTGTTGACAGGAAAACATAGAGATGTCGATGCCAAATATTTATTTGCAACCATTCAA
    ACATTATCTAGAGATGATAATTTTAAGCAATTTGATGAAAATGAATTTGATTATATTGTT
    TTTGATGAAGCACATCGTTCTGCTGCATCTACTTATCAACGAGTATTTAACTACTTCAAA
    CCTAAGTTCATGCTGGGAATGACTGCTACACCAGAAAGATCAGATGAATTAAGTATATTT
    GAACTGTTTGATTATAATATTGCATATGAAATAAGGTTACAAGCAGCATTAGAAAGTGAT
    ATTTTATGTCCATTTCATTATTTTGGTGTGACTGATTATGTACATCAAGGTATTAAAGAA
    GATGATGTAACTAAACTGAGATATTTAACTTCTGATGAAAGAGTTAATTATATTATTCAA
    AAGACAGATTACTATGGATATTCAGGTGAAATTTTACAAGGATTGATTTTTGTAAGTAGC
    AAAAAAGAAGCTTATGATTTAGCGGATAAACTAAGTTCAAAAGGGATTAAAAGTGTTGCT
    TTAACAGGAGATGATAGTGTTAATTATCGCCAAATTGTAATAGAAAAATTAAAAGAAGGT
    AAGATTAACTATATTATAACTGTAGATCTTTTTAACGAAGGTATTGATATTCCAGAAGTC
    AATCAAGTTGTAATGTTGAGACCAACAGAGTCTAGTATTATATTCATTCAGCAACTTGGT
    AGAGGATTAAGAAAAAGTTCAAATAAAGAGTATGTTACAGTAATTGATTTTATTGGTAAT
    TATAAGACAAATTATTTAATTCCGATTGCGCTTTCTGGGGATCAATCGCAAAATAAAGAT
    AATTATAAGAAGTTTTTAACGAATAACGATTCGATTAATGGAGTATCTACAATTAATTTT
    GAAGAAGTTGCTAAAAAACAGATTTACAATTCATTGGATGCAGTATCATTAAATCAAAAT
    AAATTAATATTAAAAGCTTATGAAGAAGTTGAAAATAGATTGGGACACATGCCGTTACTA
    ATGGATTTCATACAACAACATTCTATAGATCCAAGCGTTATATTTTCTAAATTTAGTAAT
    TATTACGAGTTCTTAGTGAGATATAAAAAAATAGATACATTATTGACGGAAAATGAATCT
    AAAAATCTGGTTTTCTTTTCAAGGCAAATAGCACCTGGATTGAAAAGAATCGATAGTTTA
    GTTTTAGAAGAACTTTTAAAAAATGAATTAACATATGATGAATTAAAAAATAAAATGTTG
    AACGAAGTTAAGGATATAACAGAAGATGATATAGATACTTCATTAAGAATTTTAGATTTT
    TCATTTTACAACGCAGGAATTGAAAAAATCTATGGAAGTCCCATTATAGAACGTAATGAA
    AGAATGATAAGACTAAGCGATGCATTTACTAATGCTCTAAGTAATCAAACATTTAATATG
    TTTTTAGAAGATCTTATTGAATTATCAAAGTATAACAATGAAAAATACCAAAAAGGTAAA
    AATGGACTGATTTTATATAATAAATATTCAAGAGAAGATTTTAGTAAAATATTTAATTGG
    AATAAAAATGGTTCGTCTGTAATCATGGGATACATGATAAAAAGCCAAGAAATGCCAATA
    TTTATCACTTATGATAAGCATGAGGATATAAGTGATAGCACAAAATATGAAGATGAATTT
    TTAAGTCAAGATGAATTAAAATGGTTTACTAAATCTAATAGAACGCTAGAATCAAAAGAA
    GTTCAGAAAATTTTGTCCCATAGAGCAAAAGGAATTAAAATGTATATTTTTGTACAGAAA
    AAAGATGATGATGGTATATATTTTTATTATTTAGGAACTGCTGGATATATAGAAGGTTCA
    GAAAAGCAAGATAAAATGCCGAATGGTTCAAACGTAGTAACGATGGATTTAGCACTGGAT
    AAAGCTGTAAGAGATGATATATATCGTTATCTAACAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2862

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2499 [new locus tag: SACOL_RS13095 ]
  • symbol: SACOL2499
  • description: helicase
  • length: 953
  • theoretical pI: 6.08051
  • theoretical MW: 109900
  • GRAVY: -0.407765

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 70.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 53.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 24.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 22.1)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 14)
  • TheSEED:  
    DNA/RNA helicase of DEAD/DEAH box family 
  • PFAM:
    no clan definedDUF3427; Domain of unknown function (DUF3427) (PF11907; HMM-score: 268.6)
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 120.8)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 61.7)
    PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 56.4)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 26.5)
    DUF2075; Uncharacterized conserved protein (DUF2075) (PF09848; HMM-score: 20.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.14
    • Ymax_pos: 22
    • Cmax: 0.104
    • Cmax_pos: 41
    • Smax: 0.229
    • Smax_pos: 10
    • Smean: 0.172
    • D: 0.152
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 114 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]