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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2511 [new locus tag: SACOL_RS13155 ]
  • pan locus tag?: SAUPAN006098000
  • symbol: fnbA
  • pan gene symbol?: fnbA
  • synonym:
  • product: fibronectin-binding protein A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2511 [new locus tag: SACOL_RS13155 ]
  • symbol: fnbA
  • product: fibronectin-binding protein A
  • replicon: chromosome
  • strand: -
  • coordinates: 2569440..2572496
  • length: 3057
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    GTGAAAAACAATCTTAGGTACGGCATTAGAAAACATAAATTGGGAGCAGCATCAGTATTC
    TTAGGAACAATGATCGTTGTTGGGATGGGACAAGACAAAGAAGCTGCAGCATCAGAACAA
    AAGACAACTACAGTAGAAGAAAATGGGAATTCAGCTACTGATAATAAAACAAGTGAAACA
    CAAACAACTGCAACTAACGTTAATCATATAGAAGAAACTCAATCATATAACGCAACAGTA
    ACAGAACAACCGTCAAACGCAACACAAGTAACAACTGAAGAAGCACCAAAAGCAGTACAA
    GCACCACAAACTGCACAACCAGCAAATATAGAAACAGTTAAAGAAGAGGTAGTTAAGGAA
    GAAGCGAAACCTCAAGTTAAGGAAACAACACAATCTCAAGACAATAGCGGAGATCAAAGA
    CAAGTAGATTTAACACCTAAAAAGGCTACACAAAATCAAGTCGCAGAAACACAAGTTGAA
    GTGGCACAGCCAAGAACGGCATCAGAAAGTAAGCCACGTGTGACAAGATCAGCAGATGTA
    GCGGAAGCTAAGGAAGCTAGTAACGCGAAAGTGGAAACGGGTACAGATGTAACAAGTAAA
    GTTACAGTAGAAATTGGTTCTATTGAGGGGCATAACAATACAAATAAAGTAGAACCTCAT
    GCAGGACAACGAGCGGTACTAAAATATAAGTTGAAATTTGAGAATGGTTTACATCAAGGT
    GACTACTTTGACTTTACTTTATCAAATAATGTAAATACGCATGGCGTATCAACTGCTAGA
    AAAGTACCAGAAATTAAAAATGGTTCAGTCGTAATGGCGACAGGTGAAGTTTTAGAAGGT
    GGAAAGATTAGATATACATTTACAAATGATATTGAAGATAAGGTTGATGTAACGGCTGAA
    CTAGAAATTAATTTATTTATTGATCCTAAAACTGTACAAACTAATGGAAATCAAACTATA
    ACTTCAACACTAAATGAAGAACAAACTTCAAAGGAATTAGATGTTAAATATAAAGATGGT
    ATTGGGAATTATTATGCCAATTTAAATGGATCGATTGAGACATTTAATAAAGCGAATAAT
    AGATTTTCGCATGTTGCATTTATTAAACCTAATAATGGTAAAACGACAAGTGTGACTGTT
    ACTGGAACTTTAATGAAAGGTAGTAATCAGAATGGAAATCAACCAAAAGTTAGGATATTT
    GAATACTTGGGTAATAATGAAGACATAGCGAAGAGTGTATATGCAAATACGACAGATACT
    TCTAAATTTAAAGAAGTCACAAGTAATATGAGTGGGAATTTGAATTTACAAAATAATGGA
    AGCTATTCATTGAATATAGAAAATCTAGATAAAACTTATGTTGTTCACTATGATGGAGAG
    TATTTAAATGGTACTGATGAAGTTGATTTTAGAACACAAATGGTAGGACATCCAGAGCAA
    CTTTATAAGTATTATTATGATAGAGGATATACCTTAACTTGGGATAATGGTTTAGTTTTA
    TACAGTAATAAAGCGAACGGAAATGGGAAAAATGGTCCGATTATTCAAAATAATAAATTT
    GAATATAAAGAAGATACAATTAAAGAAACTCTTACAGGTCAATATGATAAGAATTTAGTA
    ACTACTGTTGAAGAGGAATATGATTCATCAACTCTTGACATTGATTACCACACAGCTATA
    GATGGTGGAGGTGGATATGTTGATGGATACATTGAAACAATAGAAGAAACGGATTCATCA
    GCTATTGATATCGATTACCATACTGCTGTGGATAGCGAAGCAGGTCACGTTGGAGGATAC
    ACTGAGTCCTCTGAGGAATCAAATCCAATTGACTTTGAAGAATCTACACATGAAAATTCA
    AAACATCACGCTGATGTTGTTGAATATGAAGAAGATACAAACCCAGGTGGTGGTCAGGTT
    ACTACTGAGTCTAACTTAGTTGAATTTGACGAAGAGTCTACAAAAGGTATTGTAACTGGC
    GCAGTGAGCGATCATACAACAGTTGAAGATACGAAAGAATATACAACTGAAAGTAATCTG
    ATTGAATTAGTGGATGAATTACCTGAAGAGCATGGTCAAGCACAAGGACCAGTCGAGGAA
    ATTACTGAAAACAATCATCATATTTCTCATTCTGGTTTAGGAACTGAAAATGGTCACGGG
    AATTATGACGTGATTGAAGAAATCGAAGAAAATAGCCACGTTGATATTAAGAGTGAATTA
    GGTTATGAAGGTGGCCAAAATAGCGGTAACCAGTCATTCGAGGAAGACACAGAAGAAGAC
    AAACCTAAATATGAACAAGGTGGCAATATCGTAGATATCGATTTTGATAGTGTACCTCAA
    ATTCATGGTCAAAATAAAGGTAATCAGTCATTCGAGGAAGATACAGAAAAAGACAAACCT
    AAGTATGAACATGGCGGTAACATCATTGATATCGACTTCGACAGTGTGCCACATATTCAC
    GGATTCAATAAGCACACTGAAATTATTGAAGAAGATACAAATAAAGATAAACCAAGTTAT
    CAATTCGGTGGACACAATAGTGTTGACTTTGAAGAAGATACACTTCCAAAAGTAAGCGGC
    CAAAATGAAGGTCAACAAACGATTGAAGAAGATACAACACCTCCAATCGTGCCACCAACG
    CCACCGACACCAGAAGTACCAAGTGAGCCGGAAACACCAACGCCACCAACACCAGAAGTA
    CCAAGTGAGCCGGAAACACCAACACCACCGACACCAGAAGTGCCGAGTGAGCCAGAAACT
    CCAACACCGCCAACACCAGAGGTACCAGCTGAACCTGGTAAACCAGTACCACCTGCCAAA
    GAAGAACCTAAAAAGCCTTCTAAACCAGTGGAACAAGGTAAAGTAGTAACACCTGTTATT
    GAAATCAATGAAAAGGTTAAAGCAGTGGCACCAACTAAAAAACCACAATCTAAGAAATCT
    GAACTACCTGAAACAGGTGGAGAAGAATCAACAAACAAAGGTATGTTGTTCGGCGGATTA
    TTCAGCATTCTAGGTTTAGCATTATTACGCAGAAATAAAAAGAATCACAAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3057

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2511 [new locus tag: SACOL_RS13155 ]
  • symbol: FnbA
  • description: fibronectin-binding protein A
  • length: 1018
  • theoretical pI: 4.34254
  • theoretical MW: 111708
  • GRAVY: -0.922495

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 25.1)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 22)
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Fibronectin binding protein FnbA 
  • PFAM:
    Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 130.8)
    no clan definedFn_bind; Fibronectin binding repeat (PF02986; HMM-score: 104.9)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 34.9)
    YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 32.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: found LPxTG motif :LPETG
  • SignalP: Signal peptide length 37 aa
    • Ymax: 0.635
    • Ymax_pos: 37
    • Cmax: 0.493
    • Cmax_pos: 37
    • Smax: 0.88
    • Smax_pos: 34
    • Smean: 0.8
    • D: 0.699
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTTVEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAPQTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVEVAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVEPHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGEVLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELDVKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNGNQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLDKTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGNGKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGYVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIELVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKDKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA

Experimental data[edit source | edit]

  • experimental localization: Sortase substrate [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

T J Foster, M Höök
Surface protein adhesins of Staphylococcus aureus.
Trends Microbiol.: 1998, 6(12);484-8
[PubMed:10036727] [WorldCat.org] (P p)