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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2515 [new locus tag: SACOL_RS13170 ]
  • pan locus tag?: SAUPAN006131000
  • symbol: gntK
  • pan gene symbol?: gntK
  • synonym:
  • product: gluconokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2515 [new locus tag: SACOL_RS13170 ]
  • symbol: gntK
  • product: gluconokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2574439..2575992
  • length: 1554
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAATATATGATTGGTGTCGACATTGGAACGACGAGTACCAAGTCAGTCTTATATGAT
    GAAAATGGAACTTTTATCATGAAACATCAAATAGGCTATGATTTACACACACCAAACGTT
    GATGTCTCAGAAGAAAACCCAGATGAATTATTTGATGCAGTACTAATGACTATTAAATAT
    GTTATGAGAGAATCCAAAGTTAATAAAGACGACATTAAGTTTGTGTCATTTAGTGCGCAA
    ATGCATAGCTTGATTGCGATGGATCAGCAACATCAAAGATTAACAAATAATATTACTTGG
    GCAGATAACCGCGCTGCAAAATATGCAACAGTAATAAATGAAGTGCATGATGGCAATGCG
    ATTTACCAGCGAACAGGCACACCGATTCATCCTATGTCGCCATTAGCGAAGATTTTTTGG
    ATGAAACATGAATGGCAAGATGTATTTCAACGTACTGCTAAGTTTGCAGATATTAAAACA
    TACATTTTCTATCATTTATTTGATACATATATCATTGATTATTCAATGGCTTCCGCAACA
    GGGATGTTTAATTTAGAAACATTAGATTGGGATGTTGAGGCATTAGAACTGCTGGGTATT
    TCCAAGGAAATGTTGCCGGAATTAGTTCCAACAACGTATGTGATGAAAGGTATGAAAGAA
    CGTTATGCAACATTAATGGGGCTTAATAAAGATACACCGTTTGTTATTGGTGCGAGTGAT
    GGGGTCCTTTCTAATTTAGGTGTCAATAGTGTTGGTAAAGGAGAAGTTGCTGTCACAATC
    GGTACATCTGGGGCGATTCGTACTGTGATAGATAAACCGCGTACTGATTACAAAGGTAGA
    ATATTTTGTTATGTCTTAACAGAGGACCACTACGTCATCGGAGGTCCTGTAAATAATGGT
    GGTGTCGTATTGAGATGGTTGCGCGACGAGTTGCTAGCGAGTGAAGTCGAAACTGCGAAA
    CGTCTCGGTGTTGATCCTTATGATGTCTTAACTCAAATTGCAAAGCGTGTTAAACCAGGT
    GCAGATGGTTTAATATTCCATCCCTATTTAGCAGGAGAACGTGCGCCGCTTTGGAACGCA
    AATGCAAGAGGTTCATTCTTCGGTTTAACTTTATCTCATAAAAAAGAACATATGATTCGC
    GCTGCATTAGAAGGTGTTCTTTACAATTTGTATACCGTCTATCTTGCGCTTATTGAAGTA
    ATGAATGAAACACCTAAGATGATAAAAGCAACAGGTGGTTTTGCGAAAAGTGAAGTATGG
    CGTCAAATGATGTCAGATATATTTGACACAGAGTTAGTGGTTCCTGAAAGTTATGAAAGT
    TCATGCTTAGGTGCCTGCGTGCTCGGACTTAAAGCTGTAGGTGACATTGAAGATTTTTCA
    ATCGTTTCATCGATGGTCGGTGCCACAAACAATCATACGCCGATTGAAGAAAATGTCACT
    GTTTACCAAGAGATCGTATCCATTTTTATCAATTTAAGTCGTTCTTTAACAGAGAATTAT
    GAACAAATTGCAGATTTTCAACGCCAACATATCGCTGAAAATAAAACACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2515 [new locus tag: SACOL_RS13170 ]
  • symbol: GntK
  • description: gluconokinase
  • length: 517
  • theoretical pI: 5.23399
  • theoretical MW: 58094.1
  • GRAVY: -0.12089

Function[edit source | edit]

  • reaction:
    EC 2.7.1.12?  ExPASy
    GluconokinaseATP + D-gluconate = ADP + 6-phospho-D-gluconate
  • TIGRFAM:
    MetabolismEnergy metabolismSugarsgluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 1017.3)
    MetabolismEnergy metabolismSugarsxylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 338.7)
    MetabolismEnergy metabolismOtherglycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 211.4)
    MetabolismEnergy metabolismSugarsL-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 117.4)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 100.7)
    MetabolismEnergy metabolismSugarsribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 62.7)
    rhamnulokinase (TIGR02627; EC 2.7.1.5; HMM-score: 60.7)
    putative CoA-substrate-specific enzyme activase (TIGR00241; HMM-score: 21.2)
    benzoyl-CoA reductase, subunit A (TIGR02259; EC 1.3.7.8; HMM-score: 11.1)
  • TheSEED:  
    CarbohydratesMonosaccharidesD-gluconate and ketogluconates metabolism Gluconokinase (EC 2.7.1.12) 
  • PFAM:
    Actin_ATPase (CL0108) FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 241.6)
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 108.2)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 26.7)
    no clan definedDDE_Tnp_IS66_C; IS66 C-terminal element (PF13817; HMM-score: 12.8)
    Actin_ATPase (CL0108) Hydant_A_N; Hydantoinase/oxoprolinase N-terminal region (PF05378; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 11
    • Cmax: 0.114
    • Cmax_pos: 44
    • Smax: 0.203
    • Smax_pos: 2
    • Smean: 0.121
    • D: 0.118
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSEENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWADNRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKTYIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGMKERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTDYKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIAKRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYTVYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGLKAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQRQHIAENKTQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]