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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2520 [new locus tag: SACOL_RS13195 ]
  • pan locus tag?: SAUPAN006139000
  • symbol: SACOL2520
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2520 [new locus tag: SACOL_RS13195 ]
  • symbol: SACOL2520
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2579257..2581149
  • length: 1893
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    TTGACATCACTCATAAAAATCAATCGACTTAACTTAGACTTTTATAAAGGTGTAAGACAG
    GGACTGTTAATGATTATTCCTGCAATAATCGGTTACTTATGTGGTAATTTCCAATTTGGA
    TTATTAGTTGCAACCGGAACACTAGCCCATATTTATGTTTTTAAAGGTCCGTCGCGATCT
    AAGCTGCGAACTGTAATAATTTGTAATTTAGCGTTTGCAATATGTATGATGCTTGGTACG
    CTAACAGCCAAAACGCCACTCGTTTTTGGAATGACATTATTAATTGTTACGGTTATACCA
    TTTTATATATTTACTGCCTTAAAAATAGCTGGACCGTCATCGACATTCTTCATTGTGACA
    TTCAGTCTACCCATTAACTTACCTATAGCTCCCGAAGAAGCATTATATAGAGGCTTTGCG
    ATTTTAGTAGGCGGTATACTTGCCACTATGATGGTGTTAATCACGATCGTATTTTCTAAA
    AACAAAGCTGAAGAACAAGCAATTCAAAATGATTTTAAACTCATATCTAAGTTGTTACAC
    ACTTATAATGATAAATCTGCTTTTTTACAAGTAGCAAAAACAGCGGTGGATAGTTTTAAA
    GCATCTGATAAATTATTAATCACTTCTACTTCAAGTAACGATAAATTAAGTAGACGTTTC
    CAAAAATTATTATTATTACACACATCTGCCCAAGGGATTTATTCTGAACTGTTAGAGTTG
    AACGCTAAACAAATTCGACCATTGCCAGATGAGTTAATTGAAATGATGGATCATATCATT
    GCACAACTAGATAATAGTGAGGAAAATGTAAGATATTGGCGAAAAGAAGTGACAGTAACA
    GAGGAATTTCAAAATTTATTCAACCATATATTGAAAATTGATGAAATGGTGCATGCAAAT
    GAAGCGCGTATTGCGTATGAAGCAGACATGCGAAAACCTTTATATAGTAAACGCATTTAT
    CAAAATTTAACATTAGACTCTATTGTTTTTAGAAATACATTGAGATATACAGCGATTATG
    ATGATAGCGATATTTATTGCGTTAATGTTTGATTTTGAAAAAGCATACTGGATACCGTTA
    TCTGCACATACAATATTACTAGGAACATCAACTATACATGCAATCGAGAGAGGTATGGCA
    CGAGGTTTAGGTACTATTTTAGGTGTGTTAGTACTTTCAGTCATATTGTTGTTTTCAATA
    CCAACACCTGTTGCAGTAATTTTAATGGGCATTGCAGCATTGTTTACTGAAGCATTGGTG
    GGAGCAAATTATGCGATTGCAGTAGTTTTTATTACAATACAAGTTATTTTAATGAACGGA
    TTAGCATCACAGAATTTAACAATTAACATTGCGTTTCCAAGAGTTATTGACGTTGCAATG
    GGTATTGTGATTGCAATCATAGGTTTATTTGTCCTTGGACAACGTACCGCATCCGCATTG
    CTTCCTAATGTAATGGCTGAAGTTGTTCGTAAAGAAGCAACGCTCTTTCATTATTTATTT
    TCTGAAAATCAATATAAAGATGATGTGTATCAGAAAAACACGGCTATGAACCTATCTGTG
    AAATTAAATAACATGACGCAAGTTTATAATGCAGCTAATGGTGAATTATTTAGTGATAAG
    ACATTGATTCAAAATTATTATCCTAGCTTATTCGCATTAGAAGAAATTAGCTTTATGTTA
    AATAGAGCCATGGCTAATGAAGATAGACTTACAATAAATGAACAATTAATGGGCGAGTAT
    TTAGCAATATTTGAAAACATCGCTAAGCATTTCGAATTAAATACAGAATTAGAAATAAAG
    ATATTACCAGATTTACCACAGTATAATTATATTCAATCAGCAATGATGAATATTCAACAC
    AATGGTTTTCGTGAGAGAGACAAGAACGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1893

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2520 [new locus tag: SACOL_RS13195 ]
  • symbol: SACOL2520
  • description: hypothetical protein
  • length: 630
  • theoretical pI: 8.0645
  • theoretical MW: 70876.1
  • GRAVY: 0.390159

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    integral membrane protein, YccS/YhfK family (TIGR01667; HMM-score: 76.3)
    Cell structureCell envelopeOtherTIGR01666 family membrane protein (TIGR01666; HMM-score: 64.4)
  • TheSEED:  
    putative membrane protein 
  • PFAM:
    FUSC (CL0307) FUSC_2; Fusaric acid resistance protein-like (PF13515; HMM-score: 61.5)
    FUSC; Fusaric acid resistance protein family (PF04632; HMM-score: 33.4)
    FUSC-like; FUSC-like inner membrane protein yccS (PF12805; HMM-score: 5.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.108
    • Ymax_pos: 12
    • Cmax: 0.124
    • Cmax_pos: 50
    • Smax: 0.147
    • Smax_pos: 8
    • Smean: 0.119
    • D: 0.112
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTSLIKINRLNLDFYKGVRQGLLMIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVIICNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPINLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYNDKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELNAKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHANEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYWIPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALFTEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLGQRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNAANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAKHFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]