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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2702 [new locus tag: SACOL_RS14135 ]
  • pan locus tag?: SAUPAN006433000
  • symbol: hisD
  • pan gene symbol?: hisD
  • synonym:
  • product: histidinol dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2702 [new locus tag: SACOL_RS14135 ]
  • symbol: hisD
  • product: histidinol dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2774492..2775748
  • length: 1257
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    TTGCCAATGCTTAATGCACAACAATTTTTAAATCAATTTTCATTAGAAGCACCATTAGAT
    GAGTCATTGTATCCAATTATTCGCGATATTTGTCAGGAAGTTAAAGTTCATGGAGATAAG
    GCTTTGAAAATGTATAATCTAACATTCGATCATACGAAAACAGATCATTTAGAAATTAGT
    CATGAACAAATTAAAGCAGCATTTGACACATTAGATGAAAAAACAAAACAAGCATTACAA
    CAAAGTTATGAAAGAATTAAAGCATATCAAGAAAGTATTAAACAGACGAATCAACAGTTA
    GAAGAATCAGTGGAGTGTTATGAAATATACCATCCACTAGAAAGTGTCGGTATTTATGTG
    CCTGGTGGTAAAGCAAGTTATCCTTCAACGGTATTAATGACAGCGACGTTAGCACAAGTA
    GCAGGTGTGGAAAATATTGTCGTTGTGACACCACCTCAACCTAATGGTGTGTCTCAAGAA
    GTATTAGCAGCATGCTATATTACGCAAGTTGATCAAGTATTTCAAGTTGGTGGTGCTCAA
    AGTATTGCTGCGTTAACTTATGGCACAGAAACGATACCTAAAGTTGATAAGATTGTAGGT
    CCAGGCAATCAATTTGTTGCATATGCCAAGAAATATTTATTTGGACAAGTAGGTATTGAC
    CAAATAGCTGGACCAACAGAAATAGCACTTATTATTGATGAAACAGCAGATTTAGATGCC
    ATCGTATATGATGTTTTTGCACAAGCAGAACATGATGAATTAGCACGTACATATGCCATT
    AGTGAAGATGCGCAAGTCCTTAAAGATTTAGAATCACGTATTGCTAAAGCATTGCCTAAT
    GTGGACAGATACGACATTGTTTCTAAAAGTATCGCTAATCAACACTACCTTATCCATGCT
    AGTAATTTTGATGAAGCATGCCATGTCATGAATACAATCGCGCCTGAACATGCGTCGATT
    CAAACAGTAAATCCTCAACCATATATTGAAAAAGTGAAATATGTGGGTGCATTGTTTATT
    GGACATTATTCGCCAGAGGTCATAGGAGATTACGTTGCAGGTCCAAGTCATGTATTACCT
    ACAAATAGAACAGCTAGATTTACCAATGGGTTATCGGTCAATGATTTCTTAACACGGAAC
    ACGGTCATCCATTTATCAAAAGATACGTTTGAACAAATTGCTGATTCAGCACAACATATT
    GCTCATGTTGAAGCATTATACAATCACCAGCAGTCTATTTTAATACGTCAGTCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1257

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2702 [new locus tag: SACOL_RS14135 ]
  • symbol: HisD
  • description: histidinol dehydrogenase
  • length: 418
  • theoretical pI: 4.80567
  • theoretical MW: 46406
  • GRAVY: -0.17512

Function[edit source | edit]

  • reaction:
    EC 1.1.1.23?  ExPASy
    Histidinol dehydrogenaseL-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH
  • TIGRFAM:
    MetabolismAmino acid biosynthesisHistidine familyhistidinol dehydrogenase (TIGR00069; EC 1.1.1.23; HMM-score: 479.1)
  • TheSEED:  
    Amino Acids and DerivativesHistidine MetabolismHistidine Biosynthesis in plants Histidinol dehydrogenase (EC 1.1.1.23) 
    Histidine MetabolismHistidine Biosynthesis Histidinol dehydrogenase (EC 1.1.1.23) 
  • PFAM:
    ALDH-like (CL0099) Histidinol_dh; Histidinol dehydrogenase (PF00815; HMM-score: 473.9)
    no clan definedHALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.7)
    DUF4435; Protein of unknown function (DUF4435) (PF14491; HMM-score: 14)
    MbeB_N; MbeB-like, N-term conserved region (PF04837; HMM-score: 14)
    ZapB; Cell division protein ZapB (PF06005; HMM-score: 13.3)
    YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 13.1)
    no clan definedFadA; Adhesion protein FadA (PF09403; HMM-score: 12)
    YtxH; YtxH-like protein (PF12732; HMM-score: 10.5)
    DUF4404; Domain of unknown function (DUF4404) (PF14357; HMM-score: 9.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 17
    • Cmax: 0.117
    • Cmax_pos: 67
    • Smax: 0.18
    • Smax_pos: 5
    • Smean: 0.128
    • D: 0.12
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPMLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYNLTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESVECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVLAACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGIDQIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKALPNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVGALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIADSAQHIAHVEALYNHQQSILIRQS

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: SACOL1968 (HisR*), CodY
    HisR* (TF) important in Histidine biosynthesisRegPrecise    transcription unit transferred from N315 data RegPrecise
    CodY (TF) important in Amino acid metabolismRegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]