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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2712 [new locus tag: SACOL_RS14185 ]
  • pan locus tag?: SAUPAN006445000
  • symbol: drp35
  • pan gene symbol?: drp35
  • synonym:
  • product: drP35 protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2712 [new locus tag: SACOL_RS14185 ]
  • symbol: drp35
  • product: drP35 protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2784346..2785317
  • length: 972
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCACAACAAGATTTACCTACATTATTTTATAGCGGGAAGTCCAATAGTGCTGTTCCA
    ATTATATCTGAAAGTGAATTACAAACAATTACAGCTGAACCATGGCTTGAAATTTCCAAA
    AAAGGATTGCAACTAGAAGGATTGAACTTTGATCGGCAGGGACAACTCTTTTTATTGGAT
    GTATTCGAAGGCAATATTTTCAAAATCAATCCTGAAACGAAGGAAATCAAACGACCTTTT
    GTAAGTCACAAAGCGAATCCTGCAGCAATCAAAATACATAAAGATGGCCGATTATTCGTT
    TGTTATTTAGGAGATTTTAAATCTACAGGAGGCATTTTTGCAGCTACAGAAAATGGTGAC
    AACTTACAAGATATTATTGAAGATCTTTCAACAGCATATTGTATTGATGACATGGTATTT
    GATTCTAAAGGTGGATTTTATTTTACAGATTTTAGAGGATACTCTACCAATCCACTAGGA
    GGCGTTTATTATGTTTCGCCGGACTTTAGAACAGTGACGCCTATCATTCAAAATATTAGC
    GTAGCAAATGGTATTGCTTTAAGTACAGATGAAAAAGTACTATGGGTAACAGAAACAACA
    GCCAATCGATTACATCGCATTGCACTTGAAGATGATGGTGTGACGATACAACCATTTGGA
    GCTACCATACCGTACTATTTTACAGGTCATGAAGGACCAGACTCATGTTGTATTGATAGT
    GACGATAATTTATACGTAGCAATGTATGGTCAAGGTCGAGTGTTAGTTTTTAATAAAAGG
    GGTTATCCAATAGGACAAATATTGATACCAGGCCGAGATGAAGGGCATATGTTACGTTCT
    ACTCATCCGCAATTTATACCTGGAACAAATCAACTCATCATTTGTTCCAATGATATAGAA
    ATGGGCGGAGGGTCTATGCTTTATACAGTTAATGGCTTTGCAAAAGGTCATCAAAGTTTT
    CAGTTTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    972

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL2712 [new locus tag: SACOL_RS14185 ]
  • symbol: Drp35
  • description: drP35 protein
  • length: 323
  • theoretical pI: 4.71521
  • theoretical MW: 35832.2
  • GRAVY: -0.19257

Function[edit | edit source]

  • reaction:
    EC 3.1.1.-?  ExPASy
  • TIGRFAM:
  • TheSEED  :
    • Lactonase Drp35
  • PFAM:
    Beta_propeller (CL0186) SGL; SMP-30/Gluconolaconase/LRE-like region (PF08450; HMM-score: 102.2)
    and 3 more
    Arylesterase; Arylesterase (PF01731; HMM-score: 28.9)
    Str_synth; Strictosidine synthase (PF03088; HMM-score: 27.7)
    NHL; NHL repeat (PF01436; HMM-score: 23)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Ca2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003495
    • TAT(Tat/SPI): 0.000154
    • LIPO(Sec/SPII): 0.000415
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQLEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCYLGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLGGVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQPFGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGHMLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]