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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS02980 [old locus tag: SACOL0574 ]
  • pan locus tag?: SAUPAN002294000
  • symbol: SACOL_RS02980
  • pan gene symbol?: gltX
  • synonym:
  • product: glutamate--tRNA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS02980 [old locus tag: SACOL0574 ]
  • symbol: SACOL_RS02980
  • product: glutamate--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 596014..597468
  • length: 1455
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (596014..597468) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    481
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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGCGATCGTATAAGAGTAAGATATGCACCAAGTCCAACTGGGTATCTTCATATTGGT
    AATGCAAGAACAGCATTATTCAATTACTTGTATGCTAAACATTACAACGGAGATTTTGTG
    ATTCGAATTGAAGATACTGATAAAAAACGTAATTTAGAAGATGGAGAAACATCACAATTT
    GATAATCTTAAATGGTTAGGATTAGATTGGGATGAGTCTGTAGATAAAGACAATGGCTAC
    GGACCATATCGTCAATCTGAACGTCAACATATCTACCAACCATTAATAGATCAGTTACTA
    GCAGAAGATAAAGCATATAAATGCTATATGACAGAAGAAGAATTAGAAGCTGAACGTGAA
    GCGCAAATCGCTCGTGGTGAAATGCCTCGCTATGGTGGTCAACATGCGCATTTGACTGAA
    GAACAACGTCAACAATTTGAAGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCT
    CAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAAT
    GGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTA
    GCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCA
    AACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGTTTTGGT
    CATATGTCATTAATTGTTAATGAAGAACGTAAAAAGTTAAGTAAACGTGATGGGCAAATT
    TTACAATTTATTGAGCAATATCGTGACTTAGGTTATTTACCTGAAGCGTTATTTAATTTT
    ATTGCGTTATTAGGTTGGTCTCCTGAAGGTGAAGAAGAAATCTTTTCTAAAGAAGAATTT
    ATCAAAATCTTTGATGAAAAGCGTTTGTCAAAATCACCAGCATTTTTCGATAAGCAAAAA
    TTAGCATGGGTTAATAACCAATATATGAAACAAAAAGATACTGAAACAGTATTCCAATTA
    GCATTACCTCATTTAATTAAAGCAAATTTGATTCCTGAGGTGCCGTCAGAAGAGGATTTA
    TCTTGGGGACGCAAATTAATTGCGCTTTATCAAAAAGAAATGAGTTATGCCGGTGAAATT
    GTACCTTTATCAGAAATGTTCTTTAAAGAAATGCCAGCTCTTGGTGAAGAAGAACAACAA
    GTGATTAATGGAAAGCAAGTACCAGAGTTAATGACGCACTTATTCAGTAAATTAGAAGCA
    CTTGAACCATTTGAATCGGCTGAAATTAAAAAGACAATTAAAGAAGTTCAAAAAGAAACA
    GGAATAAAAGGCAAGCAATTATTTATGCCTATTCGTGTTGCTGTAACAGGCCAAATGCAT
    GGTCCTGAATTACCAAATACAATTGAAGTACTTGGTAAAGAAAAAGTGCTAAACCGTTTA
    AAACAATATAAGTAA
    60
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    1455

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS02980 [old locus tag: SACOL0574 ]
  • symbol: SACOL_RS02980
  • description: glutamate--tRNA ligase
  • length: 484
  • theoretical pI: 5.09349
  • theoretical MW: 56303.5
  • GRAVY: -0.645868

Function[edit source | edit]

  • reaction:
    EC 6.1.1.17?  ExPASy
    Glutamate--tRNA ligaseATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationglutamate--tRNA ligase (TIGR00464; EC 6.1.1.17; HMM-score: 624.4)
    Genetic information processingProtein synthesistRNA and rRNA base modificationglutamyl-queuosine tRNA(Asp) synthetase (TIGR03838; EC 6.1.1.-; HMM-score: 219.3)
    Genetic information processingProtein synthesistRNA aminoacylationglutamate--tRNA ligase (TIGR00463; EC 6.1.1.17; HMM-score: 127.9)
    Genetic information processingProtein synthesistRNA aminoacylationglutamine--tRNA ligase (TIGR00440; EC 6.1.1.18; HMM-score: 52.3)
    Genetic information processingProtein synthesistRNA aminoacylationlysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 27.2)
  • TheSEED:
  • PFAM:
    HUP (CL0039) tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 414.3)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 18.7)
    no clan definedMrr_N; Mrr N-terminal domain (PF14338; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.133
    • Ymax_pos: 11
    • Cmax: 0.122
    • Cmax_pos: 35
    • Smax: 0.201
    • Smax_pos: 1
    • Smean: 0.174
    • D: 0.149
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447206539 NCBI
  • RefSeq: WP_001283795 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGKQVPELMTHLFSKLEALEPFESAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS02980 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]