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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04015 [old locus tag: SACOL0780 ]
  • pan locus tag?: SAUPAN002608000
  • symbol: SACOL_RS04015
  • pan gene symbol?: recQ1
  • synonym:
  • product: DNA helicase RecQ

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS04015 [old locus tag: SACOL0780 ]
  • symbol: SACOL_RS04015
  • product: DNA helicase RecQ
  • replicon: chromosome
  • strand: +
  • coordinates: 802796..804577
  • length: 1782
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (802796..804577) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    481
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    ATGATGCAACAAACATTATCGCATTACTTTGGGTATGAAACGTTTCGACCAGGACAAGAA
    GAAATTATTAGCAAAGTATTAGACCATCGTAATGTGCTTGGTGTCTTACCAACTGGTGGA
    GGTAAGTCTATATGCTATCAAGTACCAGGTTTATTGTTAGGTGGTACAACAATTGTAATA
    AGTCCACTAATATCATTAATGAAAGATCAAGTGGATCAATTAAAAGCGATGGGAATTCAA
    GCTGCTTTTTTAAATAGTAGTTTGACTCAAAAAGAGCAACAACGTATTGAAAAAGCATTA
    TCAAATGGAGAAATTCAATTTTTGTATGTTGCACCAGAACGATTTGAAAACCGATATTTT
    TTAAATATGCTTCAGCGTATAAAGATTCACTTAGTCGCGTTTGATGAAGCGCATTGTATT
    TCTAAATGGGGTCATGATTTCAGGCCGAGTTACCAAAATGTTATTTCAAAAGTATTTACG
    TTACCTCAAGATTTTACAATAATAGCGTTGACAGCAACTGCCACGGTTGAAGTACAGCAA
    GATATTAGAGAAAAGTTAAATATCGCTCAAACTGATCAAATTAAAACGAGTACTAAGCGT
    AGAAACTTAATTTTTAAAGTAAATCCTACTTATCAACGTCAAAAATTTATATTGGATTAT
    ATTAAAACACACGATGAAGATGCAGGTATTATTTATTGTTCTACACGTAAGCAAGTTGAA
    GAGCTTCAAGAAGCCTTAGAAAGTCAGAAAATTGAAAGTGTTATATATCATGCAGGTTTG
    AGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTC
    GTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCAT
    TATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGAC
    GGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTAT
    TTTATAACAGTCTCTCAAGCTGATGATGACTATAAAGATAAAATGGGCGAAAAGTTAACT
    AAAATGATTCAATATACAAAAACAAAAAAATGTCTAGAAGCAACAATTGTCCATTATTTT
    GAACCGAATGAAAAATTAGAAGAATGTGAACAATGTAGTAATTGTGTTCAACAAGATAAA
    TCATATAATATGACACAAGAAGCTAAGATGATTATTAGTTGCATCGCTCGTATGAAACAA
    CAAGAGAGTTATAGTGTTATCATTCAAGTGTTAAGAGGAGAGTCAACAGATTATATTAAG
    TATAAAGGTTATGACCAAATTTCAACCCATGGTTTAATGAAAGGTTACACAACATCAGAA
    TTAAGTCACTTAATAGATGAATTAAGATTCAAAGGGTTCTTAAATGAAAATGACGAAATA
    TTAATGTGTGATACTTCAATTAAAAAATTACTCAGTAATGAAGTAGAAGTATTCACAACA
    CCATTTAAGCAAAAAGCGACTGAAAAAGTATTTATAAATACGGTTGAAGGGGTTGACCGA
    GTATTATTCAGTCAGTTGGTAGAAGTTCGTAAAAAGTTAAGTGACAAATTAACGATAGCA
    CCTGTAAGTATATTTTCTGATTACACGTTGGAGGAATTTGCTAAACGTAAGCCTGCTTCG
    AAACAAGATATGATTAATATTGATGGCGTAGGTAGTTACAAATTAAAACATTATTGTCCA
    GCATTTTTAGAAACGATTCAAAATTATAAAGCCAAAGTATAG
    60
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    1782

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS04015 [old locus tag: SACOL0780 ]
  • symbol: SACOL_RS04015
  • description: DNA helicase RecQ
  • length: 593
  • theoretical pI: 6.83017
  • theoretical MW: 68169.7
  • GRAVY: -0.376223

Function[edit source | edit]

  • reaction:
    EC 3.6.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 902.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 455.6)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 52.9)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 46)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 35.1)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 93.7)
    HTH (CL0123) RQC; RQC domain (PF09382; HMM-score: 77)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 75.2)
    HRDC-like (CL0426) HRDC; HRDC domain (PF00570; HMM-score: 61.5)
    no clan definedRecQ_Zn_bind; RecQ zinc-binding (PF16124; HMM-score: 42.7)
    DUF1033; Protein of unknown function (DUF1033) (PF06279; HMM-score: 14.5)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 70
    • Cmax: 0.108
    • Cmax_pos: 46
    • Smax: 0.19
    • Smax_pos: 67
    • Smean: 0.095
    • D: 0.107
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_000983677 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSICYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSNGEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFTLPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFILDYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDRVKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERDINLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQCSNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTHGLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATEKVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMINIDGVGSYKLKHYCPAFLETIQNYKAKV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS04015 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15

Relevant publications[edit source | edit]