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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04540 [old locus tag: SACOL0884 ]
  • pan locus tag?: SAUPAN002840000
  • symbol: SACOL_RS04540
  • pan gene symbol?: metQ1
  • synonym:
  • product: methionine ABC transporter substrate-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS04540 [old locus tag: SACOL0884 ]
  • symbol: SACOL_RS04540
  • product: methionine ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 902527..903348
  • length: 822
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (902527..903348) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAAAAATTATTTGGTCTTATTTTAGTATTAACATTTGCAGTTGTATTAGCAGCTTGC
    GGTAATGGAAACAAAAGTGGCAGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCA
    CCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTA
    GATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATT
    GACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTAC
    AAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTAT
    AAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAA
    CAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTA
    AAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAAT
    AATAAACAATCAGCAGAATTCTTACCTAAAATTTATCAAAATGAAGACGCTGATGCTGTT
    ATCATTAATTCGAACTTTGCAATCGAACAAAAACTAAATCCTAAAAAAGATTCTATTGCT
    GTAGAAAGTGCGAAAGATAATCCTTATGCAAACTTAATTGCTGTTAAAGAAGGACATCAA
    GATGATAAGAAAATCAAAGCATTAATTGAAGTATTACAATCTAAAGATATTCAAGACTTC
    ATTAATGAAAAATACAATGGTGCAGTTATTCCTGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    822

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS04540 [old locus tag: SACOL0884 ]
  • symbol: SACOL_RS04540
  • description: methionine ABC transporter substrate-binding protein
  • length: 273
  • theoretical pI: 9.49386
  • theoretical MW: 30350.7
  • GRAVY: -0.484615

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cell structureCell envelopeOtherlipoprotein, YaeC family (TIGR00363; HMM-score: 167)
  • TheSEED:
  • PFAM:
    PBP (CL0177) Lipoprotein_9; NLPA lipoprotein (PF03180; HMM-score: 290.5)
    OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 18.7)
    Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 16.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide length 29 aa
    • Ymax: 0.425
    • Ymax_pos: 29
    • Cmax: 0.334
    • Cmax_pos: 29
    • Smax: 0.907
    • Smax_pos: 8
    • Smean: 0.714
    • D: 0.538
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446654980 NCBI
  • RefSeq: WP_000732326 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAEILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKIVSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGVKIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDSIAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS04540 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]