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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05520 [old locus tag: SACOL1082 ]
  • pan locus tag?: SAUPAN003286000
  • symbol: SACOL_RS05520
  • pan gene symbol?: purH
  • synonym:
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/inosine monophosphate cyclohydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS05520 [old locus tag: SACOL1082 ]
  • symbol: SACOL_RS05520
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/inosine monophosphate cyclohydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1091409..1092887
  • length: 1479
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1091409..1092887) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    481
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    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAGAAAGCTATTTTGAGCGTATCAAATAAAACAGGTATTGTAGAGTTTGCTAAAGCG
    TTAACGCAATTAAATTATGAATTATATTCAACAGGTGGTACTAAACGTATATTAGATGAA
    GCAAATGTACCAGTTCGTTCTGTTTCAGACTTAACACATTTTCCAGAAATAATGGATGGC
    CGTGTTAAAACATTACATCCGGCTGTTCATGGTGGCATTTTAGCTGATCGAAATAAACCG
    CAGCATTTAAATGAATTATCAGAACAACATATAGATTTAATTGATATGGTAGTAGTTAAT
    TTATATCCATTCCAACAAACTGTTGCAAACCCTGATGTGACGATGGACGAAGCAATTGAA
    AATATTGATATTGGTGGTCCAACAATGTTACGTGCTGCAGCTAAAAACTATAAACATGTA
    ACAACAATTGTACATCCGGCAGATTATCAAGAAGTATTGACGCGATTAAGAAACGATTCG
    TTAGATGAGTCATATAGACAATCATTAATGATTAAAGTTTTTGAGCATACTGCAGAATAT
    GATGAAGCGATTGTACGTTTCTTTAAAGGGGATAAAGAAACTTTAAGATATGGAGAAAAT
    CCACAACAATCAGCGTATTTTGTGAGAACTTCGAATGCTAAGCACACGATTGCAGGCGCT
    AAACAATTACATGGGAAACAATTAAGCTATAACAATATTAAAGATGCAGATGCTACACTA
    GCTTTAGTTAAAAAGTTTGATACACCTGCTACAGTTGCGGTTAAACACATGAATCCATGT
    GGTGTTGGTATCGGTGACACGATAGAACAAGCATTTCAACATGCGTATGAAGCGGATAGT
    CAATCAATATTTGGTGGAATTGTTGCATTAAACCGAGCTGTAACACCTGAGTTAGCAGAG
    CAATTGCATAGTATCTTTTTGGAAGTCATTATTGCACCAAAATTTACAGATGAAGCATTA
    GATATTTTAAAACAAAAGAAGAATGTAAGATTATTAGAAATTGATATGACTATAGACAGT
    AACGAAGAAGAGTTTGTTTCAGTATCTGGCGGATATTTAGTTCAAGATAAAGACAATTAT
    GTCGTGCCAAAAGAAGAAATGAAAGTTGTTACAGAAGTAGCACCTACAGATGAACAATGG
    GAAGCAATGTTATTAGGATGGAAAGTTGTACCATCAGTAAAAAGTAATGCAATTATTTTA
    AGTAATAATAAACAAACTGTAGGTATAGGTGCTGGACAAATGAATCGTGTCGGTGCTGCT
    AAAATTGCGTTAGAGAGAGCTATTGAAATCAATGATCATGTAGCGTTAGTATCTGATGGA
    TTTTTCCCTATGGGAGATACAGTTGAACTTGCAGCACAACATGGTATAAAGGCAATTATC
    CAACCGGGTGGTTCGATTAAAGATCAAGATTCAATTGATATGGCTAATAAACATGGTATT
    GCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAA
    60
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    1479

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS05520 [old locus tag: SACOL1082 ]
  • symbol: SACOL_RS05520
  • description: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/inosine monophosphate cyclohydrolase
  • length: 492
  • theoretical pI: 5.92514
  • theoretical MW: 54347.5
  • GRAVY: -0.224187

Function[edit source | edit]

  • reaction:
    EC 3.5.4.10?  ExPASy
    IMP cyclohydrolaseIMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
    EC 2.1.2.3?  ExPASy
    Phosphoribosylaminoimidazolecarboxamide formyltransferase10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (TIGR00355; EC 2.1.2.3,3.5.4.10; HMM-score: 643.4)
  • TheSEED:
  • PFAM:
    CDA (CL0109) AICARFT_IMPCHas; AICARFT/IMPCHase bienzyme (PF01808; HMM-score: 361)
    no clan definedMGS; MGS-like domain (PF02142; HMM-score: 106.4)
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 17.7)
    EFTPs (CL0575) NSP2_assoc; Non-essential region of nsp2 of arterivirus polyprotein (PF14758; HMM-score: 15)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL_RS03755LysR family transcriptional regulator  [1] (data from MRSA252)
    SACOL_RS08930pyruvate kinase  [1] (data from MRSA252)
    SACOL_RS1169530S ribosomal protein S5  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.247
    • Ymax_pos: 21
    • Cmax: 0.193
    • Cmax_pos: 21
    • Smax: 0.402
    • Smax_pos: 7
    • Smean: 0.319
    • D: 0.275
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446631944 NCBI
  • RefSeq: WP_000709290 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDSLDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS05520 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]