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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05575 [old locus tag: SACOL1092 ]
  • pan locus tag?: SAUPAN003304000
  • symbol: SACOL_RS05575
  • pan gene symbol?: ptsI
  • synonym:
  • product: phosphoenolpyruvate--protein phosphotransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS05575 [old locus tag: SACOL1092 ]
  • symbol: SACOL_RS05575
  • product: phosphoenolpyruvate--protein phosphotransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1102054..1103772
  • length: 1719
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1102054..1103772) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    1681
    ATGTCTAAATTAATTAAAGGTATTGCCGCATCTGATGGTGTCGCAATTGCTAAAGCTTAT
    TTATTAGTTGAGCCAGACTTAACATTCGACAAAAATGAAAAAGTCACTGATGTTGAAGGA
    GAAGTTGCAAAGTTCAATAGCGCTATCGAAGCTTCTAAAGTTGAGTTAACTAAAATTAGA
    AATAATGCAGAGGTTCAACTAGGTGCTGATAAAGCTGCTATCTTTGATGCACATTTATTA
    GTTTTAGATGACCCTGAATTAATTCAACCAATCCAAGATAAGATTAAAAATGAAAACGCT
    AATGCTGCTACAGCATTAACGGATGTAACAACACAATTTGTTACAATTTTTGAATCTATG
    GATAACGAATACATGAAAGAACGTGCGGCTGATATTCGCGACGTTTCTAAACGTGTGTTA
    TCACATATTTTAGGTGTAGAATTACCGAATCCGAGTATGATTGATGAAAGCGTTGTTATT
    GTAGGGAATGACTTAACGCCATCTGATACTGCTCAATTAAATAAAGAATTCGTACAAGGT
    TTTGCTACAAACATTGGCGGAAGAACAAGTCACTCTGCAATTATGAGTCGTTCTTTAGAA
    ATTCCAGCAATTGTTGGTACAAAATCAATTACTCAAGAAGTTAAACAAGGCGACATGATT
    ATCGTAGATGGATTAAATGGTGATGTAATCGTTAATCCAACTGAAGATGAGTTAATCGCT
    TATCAAGATAAACGTGAGCGTTATTTTGCTGACAAGAAAGAATTACAAAAACTACGTGAT
    GCTGATACTGTTACAGTTGATGGTGTTCACGCAGAGCTTGCTGCAAATATTGGTACACCT
    AATGATTTGCCAGGTGTTATTGAAAATGGTGCACAAGGTATCGGCTTATATAGAACTGAG
    TTTTTATATATGGGTCGTGACCAAATGCCTACAGAAGAAGAACAATTTGAAGCTTATAAA
    GAAGTATTAGAAGCAATGGGCGGTAAACGTGTTGTTGTACGTACTTTAGATATAGGTGGA
    GATAAAGAATTATCATACTTAAACTTGCCTGAAGAAATGAATCCATTCTTAGGTTACCGT
    GCGATTCGTTTATGCCTTGCGCAACAAGATATTTTCAGACCACAGCTACGTGCATTATTA
    CGTGCATCAGTTTATGGTAAGTTAAATATCATGTTCCCAATGGTTGCAACAATTAACGAA
    TTTAGAGAAGCTAAAGCTATATTATTAGAAGAAAAAGAAAACCTTAAAAATGAAGGTCAT
    GACATTTCGGATGATATAGAATTAGGAATCATGGTAGAGATACCTGCAACAGCAGCATTA
    GCTGATGTCTTTGCTAAAGAAGTAGATTTCTTCAGTATCGGTACAAATGATTTAATTCAA
    TACACATTAGCTGCTGACCGTATGTCAGAGCGTGTATCATATCTATACCAACCATATAAC
    CCTTCAATCTTACGTTTAGTTAAACAAGTTATTGAAGCGTCACATAAAGAAGGTAAATGG
    ACAGGTATGTGTGGTGAAATGGCTGGAGATGAAACAGCTATTCCATTATTGCTTGGTTTA
    GGTTTAGATGAGTTCTCTATGAGTGCAACGTCTATTCTGAAAGCAAGAAGACAAATTAAT
    GGTTTAAGTAAAAATGAAATGACTGAACTTGCTAACCGTGCAGTCGACTGTGCAACGCAA
    GAAGAAGTTATTGAATTAGTTAACAACTACGTAAAATAA
    60
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    1719

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS05575 [old locus tag: SACOL1092 ]
  • symbol: SACOL_RS05575
  • description: phosphoenolpyruvate--protein phosphotransferase
  • length: 572
  • theoretical pI: 4.38638
  • theoretical MW: 63218.6
  • GRAVY: -0.174126

Function[edit source | edit]

  • reaction:
    EC 2.7.3.9?  ExPASy
    Phosphoenolpyruvate--protein phosphotransferasePhosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
  • TIGRFAM:
    phosphoenolpyruvate-protein phosphotransferase (TIGR01417; EC 2.7.3.9; HMM-score: 816.2)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphoenolpyruvate synthase (TIGR01418; EC 2.7.9.2; HMM-score: 209.3)
    MetabolismEnergy metabolismOtherpyruvate, phosphate dikinase (TIGR01828; EC 2.7.9.1; HMM-score: 135.6)
  • TheSEED:
  • PFAM:
    PK_TIM (CL0151) PEP-utilizers_C; PEP-utilising enzyme, TIM barrel domain (PF02896; HMM-score: 426.5)
    no clan definedPEP-utilisers_N; PEP-utilising enzyme, N-terminal (PF05524; HMM-score: 111.9)
    Leu-IlvD (CL0364) PEP-utilizers; PEP-utilising enzyme, mobile domain (PF00391; HMM-score: 83.8)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.18
    • Ymax_pos: 18
    • Cmax: 0.122
    • Cmax_pos: 18
    • Smax: 0.39
    • Smax_pos: 17
    • Smean: 0.253
    • D: 0.208
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445962199 NCBI
  • RefSeq: WP_000040054 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIVGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIELVNNYVK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS05575 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]