From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05675 [old locus tag: SACOL1111 ]
  • pan locus tag?: SAUPAN003326000
  • symbol: SACOL_RS05675
  • pan gene symbol?: potD
  • synonym:
  • product: spermidine/putrescine ABC transporter substrate-binding protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS05675 [old locus tag: SACOL1111 ]
  • symbol: SACOL_RS05675
  • product: spermidine/putrescine ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1120794..1121867
  • length: 1074
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1120794..1121867) NCBI
  • BioCyc: G1G4B-1203 BioCyc
  • MicrobesOnline: see SACOL1111

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAACGATTTTTACAACTCATTATAGGTGCATTAGTTGTGGGTATGCTTTGTCTTACT
    TTAAGTCATTGGTTTAAATCTAAAGAACAAGTGCATACAAATCAAAAAATTTACGTATAC
    AATTGGGGCGAATATATTGATCCAGAGTTAATTAAGAAATTTGAAAAAGAAACTGGCATT
    CAAGTCGTTTATGAAACTTTCGATTCAAATGAAGCGATGGAAGCCAAAATTCGCAATGGC
    GGTACACATTATGATGTTGCTTTTCCTAGTGAATATACAGTTCAAAAATTGAAAAGAGAT
    CATTTATTGTTACCAATAGATCATAATAAGGTACCTAATATTAAAAATTTAGATTCAGAT
    TATATGAATATGTCATTTGATAGAGGCAATAAATATTCTTTACCTTATTTCTTTGGAACT
    GTAGGTATTTTATATAATAAAGAAAAGTATCCAAATGAATCATTTGATAGTTGGAAGTCA
    TTGTATAATCCTAAATTTAAAAACCAAATTTTACTAGTTGACGGTGCTAGAGAGATTATA
    GGCATGAGTTTGAATAAACTTGGGTATAATCTTAATGACCGTAATTCGCACCATTTAAAA
    GAAGCAGAGCGAGATTTAACCAAACTAGCACCACAAGTAAGAGGTGTCGTAGGTGATGAA
    ATTACCATGATGCTTCAACAAAATGAAGGTAACATAGCGGTTGTTTGGAGTGGTGTTGCA
    GCACCTCTAGTGCAAGAAGGGGATAAATATAATTATGTTATTCCTAAAGAAGGATCGAAT
    TTATGGTTCGACAATATGGTAATTCCAAAAACGGCACAAAATAAAGAGGGTGCATATAAA
    TTTATGAATTTTTTATTAGATGCTAAAAATAACAAGCAAAATACAGAATTCGTAGGCTAT
    GCAACGCCAAACAAGGCTGCTCGACAATTGTTGCCTAAAGAGATTAAAGACGACCATCGT
    TTTTATCCGACTAAGAAAGAGCAAGAACGCCTTGAAGTTTATAAAGATTTAGGACCTGAA
    GTTTTAAGTGAATACAATGAAAACTTTTTGAATTTCAAAATGTCATTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1074

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS05675 [old locus tag: SACOL1111 ]
  • symbol: SACOL_RS05675
  • description: spermidine/putrescine ABC transporter substrate-binding protein
  • length: 357
  • theoretical pI: 8.29147
  • theoretical MW: 41471.1
  • GRAVY: -0.583754

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein (TIGR03261; HMM-score: 23.2)
    and 4 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids carbohydrate ABC transporter substrate-binding protein, CPR_0540 family (TIGR03850; HMM-score: 16.1)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, periplasmic molybdate-binding protein (TIGR01256; HMM-score: 16)
    Metabolism Transport and binding proteins Other ABC transporter periplasmic binding protein, thiB subfamily (TIGR01254; HMM-score: 15.9)
    Metabolism Transport and binding proteins Other thiamin/thiamin pyrophosphate ABC transporter, thiamin/thiamin pyrophospate-binding protein (TIGR01276; HMM-score: 13.8)
  • TheSEED: see SACOL1111
  • PFAM:
    PBP (CL0177) SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 123.8)
    SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 102.2)
    and 5 more
    SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 74.2)
    SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 53.4)
    Lipoprotein_8; Hypothetical lipoprotein (MG045 family) (PF02030; HMM-score: 33.1)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 16.7)
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 13.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 33 aa
    • SP(Sec/SPI): 0.49937
    • TAT(Tat/SPI): 0.004282
    • LIPO(Sec/SPII): 0.241175
    • Cleavage Site: CS pos: 33-34. VHT-NQ. Pr: 0.1597
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGEYIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHLLLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKSLYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVGDEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEGAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVYKDLGPEVLSEYNENFLNFKMSLK

Experimental data[edit | edit source]

  • experimentally validated: see SACOL1111
  • protein localization: see SACOL1111
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]