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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05735 [old locus tag: SACOL1123 ]
  • pan locus tag?: SAUPAN003341000
  • symbol: SACOL_RS05735
  • pan gene symbol?: pycA
  • synonym:
  • product: pyruvate carboxylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS05735 [old locus tag: SACOL1123 ]
  • symbol: SACOL_RS05735
  • product: pyruvate carboxylase
  • replicon: chromosome
  • strand: +
  • coordinates: 1132473..1135925
  • length: 3453
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1132473..1135925) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    TTGAAACAAATAAAAAAGTTACTTGTTGCTAACCGTGGAGAAATTGCAATTCGTATATTC
    AGAGCGGCGGCAGAATTAGACATCAGCACAGTTGCAATTTATTCGAATGAAGACAAAAGT
    TCATTACATAGATATAAAGCAGATGAATCCTATTTAGTTGGAAGTGATTTAGGTCCTGCT
    GAAAGTTATTTAAATATTGAGCGTATCATTGATGTAGCAAAACAAGCGAATGTGGATGCG
    ATTCATCCTGGCTATGGATTTTTAAGTGAAAATGAACAATTTGCGCGTCGTTGTGCAGAA
    GAAGGAATTAAATTTATTGGTCCTCATTTAGAACATTTAGATATGTTTGGAGATAAAGTT
    AAAGCTCGTACAACGGCTATCAAGGCAGATTTACCAGTTATTCCTGGTACAGACGGTCCA
    ATTAAATCATATGAATTAGCAAAAGAATTTGCAGAAGAAGCTGGTTTCCCGCTAATGATT
    AAAGCCACAAGTGGTGGCGGCGGTAAAGGTATGAGAATCGTTCGTGAAGAAAGTGAATTA
    GAAGATGCTTTCCATAGAGCAAAATCAGAAGCTGAAAAATCATTTGGTAATAGTGAAGTT
    TACATAGAAAGATACATTGATAATCCAAAGCATATTGAAGTACAAGTCATAGGTGACGAA
    CATGGAAATATCGTACACTTATTTGAACGTGATTGTTCAGTACAACGTCGTCATCAAAAA
    GTTGTAGAAGTTGCACCATCAGTTGGATTATCATCAACATTACGTCAACGTATTTGTGAT
    GCTGCAATTCAATTGATGGAAAATATTAAATATGTCAATGCAGGTACTGTTGAATTTCTA
    GTATCTGGTGACGAATTCTTCTTTATAGAAGTTAACCCTCGTGTACAAGTAGAGCATACA
    ATTACAGAGATGGTAACAGGAATTGATATTGTTAAGACACAAATTTTAGTTGCAGCAGGT
    GCCGATTTATTTGGTGAAGAGATTAATATGCCGCAACAAAAAGATATTACAACATTAGGC
    TATGCCATCCAATGTCGTATTACAACAGAAGATCCGTTAAATGATTTCATGCCGGATACT
    GGAACAATCATTGCTTATCGTTCAAGCGGGGGCTTTGGTGTACGTCTTGATGCTGGAGAT
    GGTTTCCAAGGTGCTGAGATATCACCTTATTATGATTCATTACTCGTAAAATTATCTACA
    CACGCGATATCATTTAAACAAGCAGAAGAAAAAATGGTACGCTCATTACGAGAAATGCGT
    ATTCGTGGTGTTAAAACTAATATTCCATTCTTAATTAATGTAATGAAGAATAAAAAGTTC
    ACAAGTGGTGATTACACAACTAAATTTATTGAAGAAACACCAGAACTTTTCGACATTCAG
    CCGTCTCTAGATAGAGGTACTAAAACATTAGAATATATAGGTAATGTAACAATTAATGGT
    TTCCCAAATGTTGAGAAACGTCCGAAACCAGACTATGAATTAGCATCAATTCCAACTGTA
    TCTTCAAGTAAAATCGCTTCATTTAGTGGTACGAAACAATTGCTTGATGAAGTAGGTCCA
    AAAGGTGTAGCTGAATGGGTTAAAAAGCAGGATGATGTCTTACTAACAGATACAACCTTT
    AGAGATGCACACCAATCATTATTAGCTACACGAGTTAGAACTAAGGATATGATTAATATC
    GCATCCAAAACAGCGGACGTATTTAAAGATGGTTTCTCACTAGAAATGTGGGGCGGTGCT
    ACATTTGATGTGGCATATAATTTCTTGAAGGAAAACCCATGGGAACGACTTGAACGTCTA
    CGTAAAGCTATTCCAAATGTATTATTCCAAATGTTGTTACGTGCTTCAAACGCAGTTGGT
    TATAAAAACTATCCTGATAATGTTATTCATAAATTCGTACAAGAAAGTGCTAAAGCAGGC
    ATAGATGTCTTTAGAATTTTCGATTCATTAAACTGGGTAGATCAAATGAAAGTTGCCAAT
    GAAGCAGTACAAGAAGCGGGCAAAATCTCAGAAGGTACTATTTGTTATACAGGTGACATT
    TTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAG
    TTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCT
    AAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGATTTACCAATTCATCTT
    CACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGT
    GTCGATATCATTGATACTGCTGTTGCTTCAATGAGTGGTTTAACAAGTCAGCCAAGCGCC
    AATTCGTTATATTATGCATTAAATGGCTTCCCACGCCACCTTAGAACTGATATTGAAGGT
    ATGGAGTCACTTAGTCATTATTGGTCAACTGTACGTACTTATTATTCAGACTTTGAAAGT
    GATATCAAATCACCGAATACTGAAATTTATCAACATGAAATGCCTGGTGGACAGTATTCG
    AATTTAAGTCAACAAGCTAAAAGTTTAGGTTTAGGCGAAAGATTTGATGAAGTCAAAGAT
    ATGTATCGCAGAGTGAATTTCTTATTTGGTGATATCGTAAAAGTAACACCATCGTCTAAA
    GTAGTTGGTGATATGGCACTTTATATGGTACAAAATGATCTTGATGAACAATCCGTGATT
    ACAGATGGCTATAAATTAGATTTCCCAGAATCAGTAGTGTCGTTCTTCAAAGGTGAAATA
    GGACAACCTGTAAATGGTTTTAATAAAGATTTACAAGCGGTTATTTTAAAAGGCCAAGAA
    GCACTAACAGCTCGTCCAGGTGAATATCTAGAGCCAGTTGATTTTGAAAAAGTCCGTGAG
    TTGCTTGAAGAAGAGCAACAAGGTCCTGTTACGGAGCAAGATATTATTAGTTATGTACTA
    TATCCAAAAGTATATGAACAATATATTCAAACTAGAAATCAATACGGAAACTTATCGTTA
    CTTGATACGCCTACATTCTTCTTTGGAATGCGTAATGGTGAAACAGTAGAAATCGAAATC
    GATAAAGGTAAACGATTAATTATTAAACTAGAAACGATTAGTGAACCAGATGAAAATGGT
    AATAGAACGATTTACTATGCGATGAATGGTCAAGCGAGACGTATTTACATTAAAGATGAA
    AATGTGCATACAAATGCGAACGTTAAGCCAAAAGCAGATAAGAGTAATCCAAGTCATATC
    GGTGCTCAAATGCCAGGTTCAGTAACTGAAGTCAAGGTTAGTGTAGGTGAAACTGTGAAA
    GCTAATCAGCCGTTGCTAATTACTGAAGCTATGAAAATGGAAACAACAATTCAAGCACCA
    TTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGAT
    TTATTAATCGAAATTGAAAAAGCAACTGACTAA
    60
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    3453

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS05735 [old locus tag: SACOL1123 ]
  • symbol: SACOL_RS05735
  • description: pyruvate carboxylase
  • length: 1150
  • theoretical pI: 4.9295
  • theoretical MW: 128547
  • GRAVY: -0.325913

Function[edit source | edit]

  • reaction:
    EC 6.4.1.1?  ExPASy
    Pyruvate carboxylaseATP + pyruvate + HCO3(-) = ADP + phosphate + oxaloacetate
  • TIGRFAM:
    MetabolismEnergy metabolismGlycolysis/gluconeogenesispyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 1797.3)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisacetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 547.8)
    MetabolismCentral intermediary metabolismNitrogen metabolismurea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 532.2)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsoxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 487.6)
    MetabolismEnergy metabolismOtheroxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 487.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesiscarbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 68.6)
    MetabolismEnergy metabolismPyruvate dehydrogenasedihydrolipoyllysine-residue acetyltransferase (TIGR01348; EC 2.3.1.12; HMM-score: 43.4)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 42.8)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanD-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 41.5)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 39.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 39.2)
    MetabolismTransport and binding proteinsUnknown substrateefflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 34.3)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisacetyl-CoA carboxylase, biotin carboxyl carrier protein (TIGR00531; HMM-score: 34)
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 32.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherhomocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 32)
    MetabolismCentral intermediary metabolismNitrogen fixationhomocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 32)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 28.9)
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 26.5)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylamine--glycine ligase (TIGR00877; EC 6.3.4.13; HMM-score: 25.6)
    MetabolismEnergy metabolismTCA cycledihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 23.7)
    MetabolismEnergy metabolismOther4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 23.7)
    Cellular processesCellular processesBiosynthesis of natural productscyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 23.2)
    2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase (TIGR02927; EC 2.3.1.61; HMM-score: 22.1)
    MetabolismTransport and binding proteinsOtherefflux pump membrane protein (TIGR00998; HMM-score: 18.9)
    Cellular processesCellular processesBiosynthesis of natural productsNHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 15.9)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesNHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 15.9)
    MetabolismAmino acid biosynthesisOtherpyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 15.8)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 13.9)
    MetabolismTransport and binding proteinsOthermembrane fusion protein cluster 2 protein (TIGR00999; HMM-score: 12.7)
  • TheSEED:
  • PFAM:
    ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 259.1)
    Post-HMGL (CL0597) PYC_OADA; Conserved carboxylase domain (PF02436; HMM-score: 247.1)
    PreATP-grasp (CL0483) Biotin_carb_N; Biotin carboxylase, N-terminal domain (PF00289; HMM-score: 145.6)
    Hybrid (CL0105) Biotin_carb_C; Biotin carboxylase C-terminal domain (PF02785; HMM-score: 111.8)
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 94.3)
    Hybrid (CL0105) Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 68.7)
    ATP-grasp (CL0179) ATPgrasp_Ter; ATP-grasp in the biosynthetic pathway with Ter operon (PF15632; HMM-score: 48.7)
    ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 43.4)
    Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 37.7)
    Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 37.3)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 33.2)
    Hybrid (CL0105) HlyD_D23; Barrel-sandwich domain of CusB or HlyD membrane-fusion (PF16576; HMM-score: 28)
    HlyD_3; HlyD family secretion protein (PF13437; HMM-score: 26.1)
    ATP-grasp (CL0179) RimK; RimK-like ATP-grasp domain (PF08443; HMM-score: 24)
    ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 16.2)
    GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 14.1)
    Hybrid (CL0105) RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 14)
    GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 12.9)
    POTRA (CL0191) YqfD; Putative stage IV sporulation protein YqfD (PF06898; HMM-score: 10.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: biotin
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.176
    • Ymax_pos: 24
    • Cmax: 0.115
    • Cmax_pos: 38
    • Smax: 0.486
    • Smax_pos: 21
    • Smean: 0.264
    • D: 0.21
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446732268 NCBI
  • RefSeq: WP_000809524 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEGIKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMIKATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIGDEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGTVEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFDEVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVSFFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSVTEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIEKATD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS05735 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]