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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05890 [old locus tag: SACOL1153 ]
  • pan locus tag?: SAUPAN003381000
  • symbol: SACOL_RS05890
  • pan gene symbol?: polX
  • synonym:
  • product: DNA polymerase/3'-5' exonuclease PolX

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS05890 [old locus tag: SACOL1153 ]
  • symbol: SACOL_RS05890
  • product: DNA polymerase/3'-5' exonuclease PolX
  • replicon: chromosome
  • strand: +
  • coordinates: 1161345..1163057
  • length: 1713
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1161345..1163057) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    1681
    ATGACAAAAAAAGATGTTATCAAACTATTAGAACAAATTGCTACTTATATGGAATTAAAA
    GGGGAAAATACTTTTAAAATATCAGCGTATCGAAAAGCAGCTCAAAGTCTTGAATTAGAT
    GAACGACCATTAGATGAAATATCTGATGTAACGGAGTTAAAAGGCATTGGTAAAGGTGTT
    GCAGAAGTAATCAATGATTACCGTGAGACCGGTGAATCTCAGTATTTACAGCAATTACAG
    GAAGAAGTTCCGGAAGGTCTTATTCCACTTTTGAAAATTCAAGGACTTGGAAGCAAGAAA
    ATTGCTAAGCTATATAAAGAGTTGAATATTGTTGATAAAGCGTCACTTCAAGTTGCTTGT
    GAAAATGGAAAAGTTAGTGAATTAAGCGGATTTGCTAAGAAAACGGAACAAAACATATTA
    GAAGCTGTGAAACAACTTGGTGCTAAGAAAGATAGATATCCAATTGATCAAATGAGAAGA
    CTTAATCAAGAAATCATTGATTATATAGATACATTAAATTATATCGATCAATATTCATCT
    GCAGGAAGCTTCCGTCGTTTTAAAGAAATGAGCAAAGATTTAGATTTCATAATAAGTACC
    GATAACCCAAAAGCAGTGCAGCAGCAATTATTAAATATTCCCAATAAAGTAAAAGAAGTT
    GCAGTGGGGAACACAAAAGTTTCATTAGAATTAGCGTATGATGATGAAACGATTGGTGTC
    GATTTTCGATTAATTGAACCAAGTGCTTTTTATCATACATTGCAGCATTTTACTGGGTCA
    AAAGAACATAATATAAGAATTCGACAACTTGCTAAAGCACGTGATGAAAAAGTAAGTGAA
    TATGGAATTGAACAAGCTGATGGTACATTAATTCAATATGATAGTGAAGCCAAGATATAT
    GAACATTTTAATGTGAATTTTATACCACCTGCTATGCGAGAAGATGGTAGCGAATTTGAT
    AAAGATCTAAGTAATATCATTACAATAGATGATATTAATGGTGATATTCATATGCATACA
    ACGTATAGTGATGGTGCGTTTTCTATTCGAGACATGGTAGAAGCAAATATCGCAAAAGGT
    TATAAATTCATGGTAATTACTGATCATTCACAAAGTTTACGTGTTGCTAATGGCTTACAA
    GTAGAAAGACTTTTAAGACAAAACGAAGAAATTAAGGCTTTAGATAAAGAATATAGTGAA
    ATTGATATTTATTCAGGTACAGAAATGGATATATTACCTGATGGCTCGCTGGATTATGAT
    GATGAAATTTTAGCACAACTTGATTATGTAATTGGAGCTATTCATCAAAGCTTTAACCAA
    TCAGAAGAACAAATTATGGAACGATTAGCTAATGCATGTCGCAATCCATACGTGCGACAT
    ATAGCGCATCCAACAGGGCGTATTATAGGTAGAAGAGATGGTTATAAACCGAATATTGAA
    CAATTAATGGCATTAGCTGAAGAAACGAATACAGTATTAGAAATTAATGCCAATCCACAT
    CGACTGGATCTGAGCGCTGATATCGTTCGTAAATATCCAAATGTGAAATTAACTATTAAC
    ACTGATGCGCATCATACAAATCATTTAGATTTTATGAATTATGGCGTAGCAACTGCGCAA
    AAAGGATTTGTAACAAAAGATAGAGTGATTAACGCATTATCGCGTGAAGCTTTTAAAGAC
    TTTATTGAAAATAATACAAAACTTAAGAAATAG
    60
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    1713

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS05890 [old locus tag: SACOL1153 ]
  • symbol: SACOL_RS05890
  • description: DNA polymerase/3'-5' exonuclease PolX
  • length: 570
  • theoretical pI: 5.01717
  • theoretical MW: 64869.8
  • GRAVY: -0.51807

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    histidinol phosphate phosphatase, HisJ family (TIGR01856; HMM-score: 37.9)
    Cellular processesCellular processesDNA transformationcomEA protein (TIGR01259; HMM-score: 11.6)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairHolliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 9.5)
  • TheSEED:
  • PFAM:
    HHH (CL0198) HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 72.6)
    no clan definedDNA_pol_B_thumb; DNA polymerase beta thumb (PF14791; HMM-score: 58.9)
    Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 33.3)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 25)
    NTP_transf (CL0260) DNA_pol_B_palm; DNA polymerase beta palm (PF14792; HMM-score: 21.3)
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 15.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 37
    • Cmax: 0.11
    • Cmax_pos: 37
    • Smax: 0.14
    • Smax_pos: 9
    • Smean: 0.092
    • D: 0.097
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446084087 NCBI
  • RefSeq: WP_000161942 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPLDEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIAKLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRRLNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVKEVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDEKVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDINGDIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIKALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLANACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADIVRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNTKLKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS05890 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]