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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS06475 [old locus tag: SACOL1268 ]
  • pan locus tag?: SAUPAN003549000
  • symbol: SACOL_RS06475
  • pan gene symbol?: gid
  • synonym:
  • product: FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS06475 [old locus tag: SACOL1268 ]
  • symbol: SACOL_RS06475
  • product: FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO
  • replicon: chromosome
  • strand: +
  • coordinates: 1278815..1280122
  • length: 1308
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1278815..1280122) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    1021
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    ATGACTCAAACTGTAAATGTAATAGGTGCTGGTCTTGCCGGTTCAGAAGCGGCATATCAA
    TTAGCTGAAAGAGGAATTAAAGTTAATCTAATAGAGATGAGACCTGTTAAACAAACACCA
    GCGCACCATACTGATAAATTTGCGGAACTTGTATGTTCCAATTCATTACGCGGAAATGCT
    TTAACTAATGGTGTGGGTGTTTTAAAAGAAGAAATGAGAAGATTGAATTCTATAATTATT
    GAAGCGGCTGATAAGGCACGAGTTCCAGCTGGTGGTGCATTAGCAGTTGATAGACACGAT
    TTTTCAGGTTATATTACTGAAACACTTAAAAATCATGAAAATATCACAGTTATTAATGAA
    GAAATTAATGCCATTCCAGATGGATACACAATTATCGCAACAGGACCACTTACTACAGAA
    ACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCG
    GCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGT
    TATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGC
    TTTTATGATGCAGTATTAGAAGCTGAAGTTGCGCCTGTAAATTCATTTGAAAAAGAAAAA
    TATTTCGAGGGTTGTATGCCTTTTGAAGTAATGGCAGAACGCGGACGCAAGACATTACTA
    TTTGGACCAATGAAACCAGTAGGATTAGAAGATCCAAAGACTGGGAAACGTCCTTATGCG
    GTGGTTCAATTAAGACAAGATGACGCTGCTGGTACACTCTACAATATTGTTGGCTTCCAA
    ACGCATTTAAAATGGGGAGCTCAAAAAGAAGTCATTAAATTAATTCCAGGCTTAGAAAAT
    GTTGATATTGTTAGATATGGTGTGATGCATAGAAATACCTTCATTAATTCACCGGACGTA
    TTAAACGAGAAATATGAATTGATTTCACAACCAAACATACAGTTTGCGGGACAAATGACT
    GGTGTTGAAGGTTATGTAGAAAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCG
    CATAAAATATTAGGCAAGGGTGAGGTAGTATTTCCGAGAGAAACAATGATTGGAAGTATG
    GCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTC
    GGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGCACAA
    GCTAATAGAGCTTTGGATTACTTAGAAAATTTCAAAAAAACTTTATAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS06475 [old locus tag: SACOL1268 ]
  • symbol: SACOL_RS06475
  • description: FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO
  • length: 435
  • theoretical pI: 5.53912
  • theoretical MW: 48370.9
  • GRAVY: -0.293793

Function[edit source | edit]

  • reaction:
    EC 2.1.1.74?  ExPASy
    Methylenetetrahydrofolate--tRNA-(uracil54-C5)-methyltransferase (FADH2-oxidizing)5,10-methylenetetrahydrofolate + uracil54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil54 in tRNA + FAD
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA:m(5)U-54 methyltransferase (TIGR00137; EC 2.1.1.74; HMM-score: 723.4)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (TIGR00136; HMM-score: 77.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 29.2)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 27.8)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 21.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamineglycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 20.3)
    MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, monomeric form (TIGR01377; HMM-score: 17.8)
    lycopene cyclase family protein (TIGR01790; HMM-score: 16.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherphytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 16.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 15.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 15)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 14.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiazole biosynthesis enzyme (TIGR00292; HMM-score: 14.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherC-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 14.5)
    MetabolismCentral intermediary metabolismNitrogen metabolismnitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 14.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase (TIGR02028; HMM-score: 13.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 13.6)
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 13.6)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 13)
    MetabolismEnergy metabolismTCA cyclesuccinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01811; EC 1.3.99.1; HMM-score: 12.4)
    Cellular processesCellular processesDetoxificationCoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 12.2)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 12.1)
    Cellular processesCellular processesDetoxificationalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 12)
    Cellular processesCellular processesAdaptations to atypical conditionsalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 12)
    MetabolismEnergy metabolismAnaerobicglycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 11.3)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 375.3)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 25.7)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 25.4)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 21.9)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 21.4)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 21.4)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 20.4)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 17.8)
    Thi4; Thi4 family (PF01946; HMM-score: 15)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 14.9)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 14.3)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 14)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 13.5)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:
  • protein partners:
    SACOL_RS00045DNA gyrase subunit B  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.138
    • Ymax_pos: 44
    • Cmax: 0.126
    • Cmax_pos: 44
    • Smax: 0.236
    • Smax_pos: 41
    • Smean: 0.112
    • D: 0.128
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446117399 NCBI
  • RefSeq: WP_000195254 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHTDKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFSGYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDAAAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEKEKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQFAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKNFQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS06475 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]