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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS06740 [old locus tag: SACOL1323 ]
  • pan locus tag?: SAUPAN003618000
  • symbol: SACOL_RS06740
  • pan gene symbol?: miaA
  • synonym:
  • product: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS06740 [old locus tag: SACOL1323 ]
  • symbol: SACOL_RS06740
  • product: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA
  • replicon: chromosome
  • strand: +
  • coordinates: 1341464..1342399
  • length: 936
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1341464..1342399) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAATAAAAATAAGCCTTTTATTGTAGTAATTGTGGGGCCAACTGCTTCAGGTAAAACA
    GAGCTTAGCATCGAACTCGCGAAGCGTATCAATGGTGAAATCATAAGCGGTGACTCTATG
    CAAGTCTACAAACATATGAATATTGGAACTGCAAAAGTAACACCTGAAGAAATGGATGGT
    ATTCCACATCATTTAATTGATATCTTGAATCCTGATGATACATTTTCAGCATATGAATTC
    AAGCGATTAGCAGAAGATTTAATTACTGATATAACGAATAGAGGTAAAGTTCCAATCATA
    GCAGGTGGAACAGGCTTATATATTCAATCATTAATATATAATTATGAATTAGAAGATGAA
    ACAGTTACACCTGCACAATTATCCATAGTTAAACAAAAGTTATCTGCATTAGAACATTTA
    GATAATCAGCAACTACACGATTATTTAGCTCAATTTGATGCGGTTTCTGCAGAAAATATT
    CACCCTAACAACCGCCAAAGAGTGTTGCGCGCTATTGAATATTATTTAAAAACAAAAAAA
    CTTTTGAGTAATCGCAAGAAAGTGCAACAATTTACTGAAAATTATGATACATTATTATTA
    GGGATTGAAATGTCGCGTAAAACATTATATTCAAGAATAAATAAACGTGTTGATATTATG
    TTGGATCACGGATTATTTAGAGAAGTGCAACAACTTGTTGAACAAGGCTATGAATCTTGC
    CAAAGTATGCAAGCTATTGGATATAAAGAATTAATACCTGTGATTAACGGACAAATGATT
    TATGAAGATGCTGTCAATGATTTAAAGCAACATTCACGCCAATATGCAAAACGACAAATG
    ACATGGTTCAAGAATAAAATGAGTGTTCATTGGTTAGATAAAGAAAATATGTCACTTCAA
    ATGATGTTAGATGAGATTACAACCCAGATTAAGTAA
    60
    120
    180
    240
    300
    360
    420
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    540
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    936

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS06740 [old locus tag: SACOL1323 ]
  • symbol: SACOL_RS06740
  • description: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA
  • length: 311
  • theoretical pI: 6.80558
  • theoretical MW: 35868
  • GRAVY: -0.431833

Function[edit source | edit]

  • reaction:
    EC 2.5.1.75?  ExPASy
    tRNA dimethylallyltransferaseDimethylallyl diphosphate + adenine37 in tRNA = diphosphate + N6-dimethylallyladenine37 in tRNA
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA dimethylallyltransferase (TIGR00174; EC 2.5.1.75; HMM-score: 324.5)
    carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 15.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionscytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 13.4)
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 13.2)
    MetabolismCentral intermediary metabolismOther2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 10.8)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) IPPT; IPP transferase (PF01715; HMM-score: 267.1)
    IPT; Isopentenyl transferase (PF01745; HMM-score: 34.6)
    AAA_33; AAA domain (PF13671; HMM-score: 21.1)
    AAA_18; AAA domain (PF13238; HMM-score: 16.1)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 14.5)
    Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 14.3)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 14.3)
    Hydin_ADK; Hydin Adenylate kinase-like domain (PF17213; HMM-score: 13.7)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.19
    • Ymax_pos: 11
    • Cmax: 0.168
    • Cmax_pos: 28
    • Smax: 0.474
    • Smax_pos: 1
    • Smean: 0.338
    • D: 0.247
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_001548613 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENIHPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS06740 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 1.65 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]