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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07395 [old locus tag: SACOL1450 ]
  • pan locus tag?: SAUPAN003833000
  • symbol: SACOL_RS07395
  • pan gene symbol?: arlS
  • synonym:
  • product: two-component sensor histidine kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS07395 [old locus tag: SACOL1450 ]
  • symbol: SACOL_RS07395
  • product: two-component sensor histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1464055..1465410
  • length: 1356
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1464055..1465410) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGACAAAACGTAAATTGCGCAATAACTGGATTATTGTTACCACGATGATTACGTTTGTC
    ACGATATTTTTGTTTTGTTTAATTATTATTTTTTTCTTGAAAGATACACTGCATAATAGT
    GAGCTTGATGATGCAGAACGAAGCTCAAGCGATATTAATAATTTATTTCATTCTAAGCCT
    GTTAAAGATATATCTGCATTAGACTTGAATGCATCTTTAGGTAATTTTCAAGAGATAATT
    ATTTATGATGAGCATAATAATAAATTATTTGAGACATCGAATGATAACACAGTGAGAGTT
    GAACCAGGTTATGAACACCGTTATTTTGACCGCGTAATAAAAAAACGCTATAAAGGCATT
    GAATATTTAATTATTAAAGAACCAATTACAACGCAAGATTTCAAAGGGTATAGCTTGTTA
    ATTCATTCACTAGAAAATTATGATAACATCGTAAAATCATTGTATATCATTGCGCTGGCA
    TTTGGAGTGATTGCAACAATTATAACTGCCACAATCAGTTATGTATTTTCAACACAAATT
    ACTAAACCGCTTGTCAGTTTATCAAATAAAATGATTGAGATTCGACGAGATGGTTTTCAA
    AATAAATTGCAATTAAATACAAATTATGAAGAAATAGATAATTTAGCAAATACGTTTAAT
    GAGATGATGAGCCAAATTGAAGAATCATTTAATCAACAAAGACAATTTGTTGAAGATGCG
    TCACATGAATTACGAACACCATTACAAATTATTCAAGGTCATTTAAATTTGATTCAGCGA
    TGGGGAAAAAAAGACCCAGCAGTATTAGAAGAATCGTTAAATATTTCTATTGAAGAAATG
    AATCGTATCATAAAATTAGTCGAAGAATTACTTGAATTGACTAAAGGAGATGTAAATGAC
    ATTTCTTCTGAAGCACAGACCGTGCATATTAATGATGAAATTCGCTCGCGAATACACTCA
    TTAAAACAATTGCATCCTGATTATCAATTTGATACGGATCTGACATCTAAAAATCTAGAA
    ATTAAAATGAAACCTCATCAATTCGAACAATTATTTTTAATCTTTATTGATAATGCAATC
    AAATATGATGTGAAGAATAAGAAAATTAAAGTTAAGACAAGGTTAAAAAATAAGCAAAAA
    ATAATTGAAATTACAGATCATGGAATTGGTATTCCAGAGGAAGATCAAGATTTCATTTTT
    GATCGCTTTTATCGAGTGGATAAATCTCGTTCAAGAAGTCAAGGCGGTAATGGACTCGGA
    TTATCTATTGCTCAAAAAATCATTCAATTAAACGGAGGATCGATTAAAATTAAAAGTGAA
    ATTAACAAAGGAACAACGTTTAAAATCATATTTTAA
    60
    120
    180
    240
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    1020
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    1140
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    1260
    1320
    1356

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS07395 [old locus tag: SACOL1450 ]
  • symbol: SACOL_RS07395
  • description: two-component sensor histidine kinase
  • length: 451
  • theoretical pI: 6.97701
  • theoretical MW: 52399.7
  • GRAVY: -0.33592

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 197.2)
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 177.3)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 91)
    Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 89)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 51.5)
    Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 51.5)
    MetabolismCentral intermediary metabolismNitrogen fixationnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 49.9)
    Signal transductionRegulatory functionsProtein interactionsnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 49.9)
    Cellular processesCellular processesSporulation and germinationanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.1)
    Signal transductionRegulatory functionsProtein interactionsanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.1)
  • TheSEED:
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 88.5)
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 51.9)
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 25.6)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 22.1)
    no clan definedHAMP; HAMP domain (PF00672; HMM-score: 13.7)
    KaiA_RbsU (CL0637) RsbU_N; Phosphoserine phosphatase RsbU, N-terminal domain (PF08673; HMM-score: 13.4)
    no clan definedClusterin; Clusterin (PF01093; HMM-score: 13)
    DUF4199; Protein of unknown function (DUF4199) (PF13858; HMM-score: 11.9)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 11.4)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 11.2)
    no clan definedMukF_C; MukF C-terminal domain (PF17193; HMM-score: 11.1)
    DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 8.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.206
    • Ymax_pos: 40
    • Cmax: 0.135
    • Cmax_pos: 40
    • Smax: 0.43
    • Smax_pos: 36
    • Smean: 0.251
    • D: 0.223
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

  • GI: 446088946 NCBI
  • RefSeq: WP_000166801 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDDAERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEPGYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALAFGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANTFNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNISIEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDLTSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIPEEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKIIF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS07395 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]