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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07560 [old locus tag: SACOL1483 ]
  • pan locus tag?: SAUPAN003900000
  • symbol: SACOL_RS07560
  • pan gene symbol?:
  • synonym:
  • product: RNA methyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS07560 [old locus tag: SACOL1483 ]
  • symbol: SACOL_RS07560
  • product: RNA methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1523994..1525139
  • length: 1146
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1523994..1525139) NCBI
  • BioCyc: G1G4B-1593 BioCyc
  • MicrobesOnline: see SACOL1483

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTTTCAATTACTTGCAGTTTGTCCGATGGGATTAGAAGCTGTTGTTGCTAGGGAAATT
    CAAGAATTAGGCTATGAAACAAATGTTGAAAATGGTCGTATATTTTTTGAAGGAGACGCA
    AGTGCAATTGTAAAGGCAAATTTATGGTTGCGCACAGCAGACCGAATCAAAATTGTTGTT
    GGACGTTTTAACGCAACAACGTTTGACGAATTATTCGAACAAACCAAAGCGCTCCCTTGG
    GAATCTATAATTGATAAAGAGGGTAACTTCCCAGTTCAAGGTAGAAGCGTTAAATCAACA
    CTACATAGTGTGCCAGATTGTCAGGCGATAACTAAGAAAGCTATTGTTGAACGATTACGT
    CGCGCATATAACGAAAAAGGTTGGTTAAATGAATCAGGTGCCAAATACCCTGTTGAAGTT
    GCCATTTTAAAAGATAATGTATTATTGACTATCGACACATCAGGTTCTGGTTTGAACAGA
    CGTGGTTATAGATTAGCACAAGGTGAAGCACCAATTAAAGAAACGTTGGCAGCAAGTTTA
    ATCCGTCTTGCAAACTGGAAAGGTGATACACCTTTAATTGATCCATTTTGCGGTTCGGGT
    ACAATAGCTATAGAAGCTTGTTTAATTGCTCAAAATATCGCACCTGGTTTTAATCGCGAG
    TTCGTATCAGAGCAATGGAACATCATGCCAGCAAATATTTATGATGATTACCGTGATGAA
    GCGGATAAGATGGCTGATTATGATAAAGAAATCGAAGTATATGCTTCTGATATCGATCCA
    GAAATGGTAGAGATTGCTAAGCGTAACGCTGAAGAAGTTGGGTTGTCTGATATTATTAAA
    TTTAGTGTAAAAGATGTCAATACATTAACAATTGATACAGAAGAACCGGTGGCGTTAATT
    GGAAATCCTCCATATGGTGAACGTATTGGTGATCGTGAAGAAGTTGAAGAAATGTACCGT
    TATATTGGTAAACTAATGAAACAACATCCATTTTTATCTACATACATTTTAACAAGTAAT
    AAAGAATTTGAATATTTAGTAGATCGTAAAGCAACAAAGCGACGTAAGTTATTTAATGGA
    TATATAGAATGTACTTATTATCAATACTGGGGTAAAAAGACAGAACGTAAAACTATTGAA
    AACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1146

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS07560 [old locus tag: SACOL1483 ]
  • symbol: SACOL_RS07560
  • description: RNA methyltransferase
  • length: 381
  • theoretical pI: 4.78856
  • theoretical MW: 43333
  • GRAVY: -0.376115

Function[edit | edit source]

  • TIGRFAM:
    Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 51.9)
    and 10 more
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 32.6)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 30.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 19.8)
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 19)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 19)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 16.2)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 15.6)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 14.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 12.4)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 12.1)
  • TheSEED: see SACOL1483
  • PFAM:
    NADP_Rossmann (CL0063) UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 196.7)
    and 13 more
    no clan defined THUMP; THUMP domain (PF02926; HMM-score: 45.4)
    NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 23.8)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 21.2)
    N6_N4_Mtase; DNA methylase (PF01555; HMM-score: 17.1)
    no clan defined DUF5027; Domain of unknown function (DUF5027) (PF16430; HMM-score: 15.9)
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 15.8)
    Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 15)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.8)
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 14.4)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 13.1)
    Met_10; Met-10+ like-protein (PF02475; HMM-score: 12.9)
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 12.1)
    TRM; N2,N2-dimethylguanosine tRNA methyltransferase (PF02005; HMM-score: 11.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.047727
    • TAT(Tat/SPI): 0.000694
    • LIPO(Sec/SPII): 0.008568
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIVKANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLHSVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNRRGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFNREFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLSDIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLSTYILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN

Experimental data[edit | edit source]

  • experimentally validated: see SACOL1483
  • protein localization: see SACOL1483
  • quantitative data / protein copy number per cell: see SACOL1483
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]