From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08615 [old locus tag: SACOL1689 ]
  • pan locus tag?: SAUPAN004239000
  • symbol: SACOL_RS08615
  • pan gene symbol?: relA
  • synonym:
  • product: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS08615 [old locus tag: SACOL1689 ]
  • symbol: SACOL_RS08615
  • product: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 1718109..1720298
  • length: 2190
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1718109..1720298) NCBI
  • BioCyc: G1G4B-1807 BioCyc
  • MicrobesOnline: see SACOL1689

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAACGAATATCCATATAGTGCAGACGAAGTTCTTCACAAAGCAAAATCATATTTG
    TCAGCAGATGAATATGAGTATGTTTTAAAAAGCTATCATATTGCTTATGAAGCACATAAA
    GGTCAGTTCCGAAAAAACGGATTACCATACATTATGCATCCTATACAAGTTGCAGGTATT
    TTAACAGAAATGCGATTAGACGGACCGACGATTGTCGCAGGTTTTTTGCATGATGTAATT
    GAAGATACACCGTATACATTTGAAGATGTAAAAGAAATGTTCAATGAAGAAGTTGCTCGA
    ATTGTTGATGGTGTGACGAAGCTTAAAAAAGTAAAATACCGCTCAAAAGAAGAACAACAA
    GCTGAAAATCATCGCAAGTTATTTATTGCGATTGCCAAAGATGTACGCGTAATTTTGGTG
    AAATTAGCAGACAGATTACATAATATGCGTACCTTGAAAGCCATGCCGCGCGAAAAACAA
    ATTAGAATTTCTCGAGAAACATTAGAAATTTATGCACCATTAGCACATCGTCTTGGTATT
    AATACAATCAAATGGGAACTAGAAGATACGGCTCTTCGTTATATTGATAATGTGCAATAT
    TTTAGAATAGTCAATTTAATGAAGAAGAAACGTAGTGAACGTGAAGCGTATATCGAAACG
    GCTATTGATAGAATACGTACTGAAATGGACCGAATGAATATCGAAGGCGATATAAATGGT
    AGACCTAAACATATTTACAGTATTTATCGGAAAATGATGAAGCAGAAAAAACAATTTGAT
    CAAATTTTTGATTTGTTGGCGATACGTGTTATTGTCAATTCTATTAATGATTGTTATGCG
    ATACTTGGGTTGGTGCATACGTTATGGAAACCGATGCCAGGACGTTTTAAAGATTATATT
    GCAATGCCTAAACAAAATTTGTATCAGTCATTGCATACTACAGTAGTAGGCCCAAATGGA
    GACCCGCTCGAAATCCAAATACGAACGTTTGATATGCACGAAATTGCTGAGCATGGTGTT
    GCAGCACACTGGGCTTACAAAGAAGGTAAAAAAGTAAGTGAAAAAGATCAAACTTATCAA
    AATAAGTTAAATTGGTTAAAAGAATTAGCTGAAGCGGATCATACATCGTCTGACGCTCAA
    GAATTTATGGAAACCTTAAAATATGACTTACAGAGTGACAAAGTATACGCATTTACCCCA
    GCGAGTGATGTTATTGAGTTGCCATATGGTGCTGTGCCGATTGATTTTGCTTATGCGATT
    CACAGTGAAGTAGGTAATAAGATGATTGGTGCCAAGGTGAATGGCAAAATTGTACCAATT
    GACTATATTTTACAAACAGGCGATATTGTTGAAATACGTACTAGTAAACATTCATATGGA
    CCAAGTCGTGATTGGTTGAAAATTGTTAAATCGTCTAGTGCCAAAGGTAAAATTAAAAGT
    TTCTTCAAAAAACAAGATCGTTCATCTAATATTGAAAAAGGCCGAATGATGGTTGAAGCT
    GAAATAAAAGAGCAAGGATTTAGAGTCGAAGATATTTTGACAGAGAAAAATATTCAGGTT
    GTTAATGAAAAATATAACTTTGCAAATGAAGATGATTTATTCGCAGCTGTAGGATTTGGC
    GGCGTGACATCCTTACAGATTGTTAATAAATTAACTGAAAGACAACGTATTTTAGATAAA
    CAACGTGCTTTAAATGAAGCACAAGAAGTTACGAAATCATTGCCTATTAAAGACAACATC
    ATTACTGATAGTGGTGTCTATGTAGAAGGTTTAGAAAATGTACTTATCAAGTTGTCAAAA
    TGTTGTAATCCTATACCAGGTGATGATATTGTAGGTTATATCACCAAAGGTCACGGTATT
    AAAGTACATCGCACTGATTGCCCAAATATTAAGAACGAAACTGAACGACTAATTAATGTT
    GAATGGGTAAAATCAAAAGACGCAACTCAAAAATATCAGGTTGATTTAGAGGTAACTGCG
    TATGACCGAAATGGCTTGTTGAATGAAGTACTACAAGCTGTTAGCTCGACAGCCGGCAAT
    TTAATTAAAGTTTCAGGACGTTCAGATATTGATAAAAATGCAATAATAAATATTAGTGTC
    ATGGTGAAAAACGTGAATGATGTTTATCGTGTGGTAGAAAAGATCAAACAACTTGGTGAT
    GTTTATACAGTAACAAGAGTTTGGAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2190

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS08615 [old locus tag: SACOL1689 ]
  • symbol: SACOL_RS08615
  • description: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
  • length: 729
  • theoretical pI: 8.59224
  • theoretical MW: 83693.5
  • GRAVY: -0.438683

Function[edit | edit source]

  • reaction:
    EC 2.7.6.5?  ExPASy
    GTP diphosphokinase ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions RelA/SpoT family protein (TIGR00691; HMM-score: 802.9)
    and 3 more
    TIGR04552 family protein (TIGR04552; HMM-score: 15.8)
    Unknown function General TIGR04562 family protein (TIGR04562; HMM-score: 14.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family (TIGR00042; EC 3.6.1.-; HMM-score: 13.1)
  • TheSEED: see SACOL1689
  • PFAM:
    HD_PDEase (CL0237) HD_4; HD domain (PF13328; HMM-score: 160.3)
    NTP_transf (CL0260) RelA_SpoT; Region found in RelA / SpoT proteins (PF04607; HMM-score: 136.5)
    and 5 more
    Ubiquitin (CL0072) TGS; TGS domain (PF02824; HMM-score: 72.1)
    ACT (CL0070) ACT_4; ACT domain (PF13291; HMM-score: 67.3)
    HD_PDEase (CL0237) HD; HD domain (PF01966; HMM-score: 46.1)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 30.2)
    Ubiquitin (CL0072) Ub-Mut7C; Mut7-C ubiquitin (PF14451; HMM-score: 14.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005505
    • TAT(Tat/SPI): 0.000365
    • LIPO(Sec/SPII): 0.001436
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFRKNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIVDGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQIRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYIETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSINDCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHEIAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQSDKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIVEIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFRVEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNEAQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHRTDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLIKVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN

Experimental data[edit | edit source]

  • experimentally validated: see SACOL1689
  • protein localization: see SACOL1689
  • quantitative data / protein copy number per cell: see SACOL1689
  • interaction partners:
    SACOL_RS00185PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA  [1] (data from MRSA252)
    SACOL_RS03075elongation factor G  [1] (data from MRSA252)
    SACOL_RS04805D-alanine--poly(phosphoribitol) ligase subunit 2  [1] (data from MRSA252)
    SACOL_RS1161530S ribosomal protein S9  [1] (data from MRSA252)
    SACOL_RS1168550S ribosomal protein L15  [1] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]