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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08615 [old locus tag: SACOL1689 ]
  • pan locus tag?: SAUPAN004239000
  • symbol: SACOL_RS08615
  • pan gene symbol?: relA
  • synonym:
  • product: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08615 [old locus tag: SACOL1689 ]
  • symbol: SACOL_RS08615
  • product: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 1718109..1720298
  • length: 2190
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1718109..1720298) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    ATGAACAACGAATATCCATATAGTGCAGACGAAGTTCTTCACAAAGCAAAATCATATTTG
    TCAGCAGATGAATATGAGTATGTTTTAAAAAGCTATCATATTGCTTATGAAGCACATAAA
    GGTCAGTTCCGAAAAAACGGATTACCATACATTATGCATCCTATACAAGTTGCAGGTATT
    TTAACAGAAATGCGATTAGACGGACCGACGATTGTCGCAGGTTTTTTGCATGATGTAATT
    GAAGATACACCGTATACATTTGAAGATGTAAAAGAAATGTTCAATGAAGAAGTTGCTCGA
    ATTGTTGATGGTGTGACGAAGCTTAAAAAAGTAAAATACCGCTCAAAAGAAGAACAACAA
    GCTGAAAATCATCGCAAGTTATTTATTGCGATTGCCAAAGATGTACGCGTAATTTTGGTG
    AAATTAGCAGACAGATTACATAATATGCGTACCTTGAAAGCCATGCCGCGCGAAAAACAA
    ATTAGAATTTCTCGAGAAACATTAGAAATTTATGCACCATTAGCACATCGTCTTGGTATT
    AATACAATCAAATGGGAACTAGAAGATACGGCTCTTCGTTATATTGATAATGTGCAATAT
    TTTAGAATAGTCAATTTAATGAAGAAGAAACGTAGTGAACGTGAAGCGTATATCGAAACG
    GCTATTGATAGAATACGTACTGAAATGGACCGAATGAATATCGAAGGCGATATAAATGGT
    AGACCTAAACATATTTACAGTATTTATCGGAAAATGATGAAGCAGAAAAAACAATTTGAT
    CAAATTTTTGATTTGTTGGCGATACGTGTTATTGTCAATTCTATTAATGATTGTTATGCG
    ATACTTGGGTTGGTGCATACGTTATGGAAACCGATGCCAGGACGTTTTAAAGATTATATT
    GCAATGCCTAAACAAAATTTGTATCAGTCATTGCATACTACAGTAGTAGGCCCAAATGGA
    GACCCGCTCGAAATCCAAATACGAACGTTTGATATGCACGAAATTGCTGAGCATGGTGTT
    GCAGCACACTGGGCTTACAAAGAAGGTAAAAAAGTAAGTGAAAAAGATCAAACTTATCAA
    AATAAGTTAAATTGGTTAAAAGAATTAGCTGAAGCGGATCATACATCGTCTGACGCTCAA
    GAATTTATGGAAACCTTAAAATATGACTTACAGAGTGACAAAGTATACGCATTTACCCCA
    GCGAGTGATGTTATTGAGTTGCCATATGGTGCTGTGCCGATTGATTTTGCTTATGCGATT
    CACAGTGAAGTAGGTAATAAGATGATTGGTGCCAAGGTGAATGGCAAAATTGTACCAATT
    GACTATATTTTACAAACAGGCGATATTGTTGAAATACGTACTAGTAAACATTCATATGGA
    CCAAGTCGTGATTGGTTGAAAATTGTTAAATCGTCTAGTGCCAAAGGTAAAATTAAAAGT
    TTCTTCAAAAAACAAGATCGTTCATCTAATATTGAAAAAGGCCGAATGATGGTTGAAGCT
    GAAATAAAAGAGCAAGGATTTAGAGTCGAAGATATTTTGACAGAGAAAAATATTCAGGTT
    GTTAATGAAAAATATAACTTTGCAAATGAAGATGATTTATTCGCAGCTGTAGGATTTGGC
    GGCGTGACATCCTTACAGATTGTTAATAAATTAACTGAAAGACAACGTATTTTAGATAAA
    CAACGTGCTTTAAATGAAGCACAAGAAGTTACGAAATCATTGCCTATTAAAGACAACATC
    ATTACTGATAGTGGTGTCTATGTAGAAGGTTTAGAAAATGTACTTATCAAGTTGTCAAAA
    TGTTGTAATCCTATACCAGGTGATGATATTGTAGGTTATATCACCAAAGGTCACGGTATT
    AAAGTACATCGCACTGATTGCCCAAATATTAAGAACGAAACTGAACGACTAATTAATGTT
    GAATGGGTAAAATCAAAAGACGCAACTCAAAAATATCAGGTTGATTTAGAGGTAACTGCG
    TATGACCGAAATGGCTTGTTGAATGAAGTACTACAAGCTGTTAGCTCGACAGCCGGCAAT
    TTAATTAAAGTTTCAGGACGTTCAGATATTGATAAAAATGCAATAATAAATATTAGTGTC
    ATGGTGAAAAACGTGAATGATGTTTATCGTGTGGTAGAAAAGATCAAACAACTTGGTGAT
    GTTTATACAGTAACAAGAGTTTGGAACTAG
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08615 [old locus tag: SACOL1689 ]
  • symbol: SACOL_RS08615
  • description: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
  • length: 729
  • theoretical pI: 8.59224
  • theoretical MW: 83693.5
  • GRAVY: -0.438683

Function[edit source | edit]

  • reaction:
    EC 2.7.6.5?  ExPASy
    GTP diphosphokinaseATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
  • TIGRFAM:
    Cellular processesCellular processesAdaptations to atypical conditionsRelA/SpoT family protein (TIGR00691; HMM-score: 802.9)
    TIGR04552 family protein (TIGR04552; HMM-score: 15.8)
    Unknown functionGeneralTIGR04562 family protein (TIGR04562; HMM-score: 14.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairnon-canonical purine NTP pyrophosphatase, RdgB/HAM1 family (TIGR00042; EC 3.6.1.-; HMM-score: 13.1)
  • TheSEED:
  • PFAM:
    HD_PDEase (CL0237) HD_4; HD domain (PF13328; HMM-score: 160.3)
    NTP_transf (CL0260) RelA_SpoT; Region found in RelA / SpoT proteins (PF04607; HMM-score: 136.5)
    Ubiquitin (CL0072) TGS; TGS domain (PF02824; HMM-score: 72.1)
    ACT (CL0070) ACT_4; ACT domain (PF13291; HMM-score: 67.3)
    HD_PDEase (CL0237) HD; HD domain (PF01966; HMM-score: 46.1)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 30.2)
    Ubiquitin (CL0072) Ub-Mut7C; Mut7-C ubiquitin (PF14451; HMM-score: 14.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL_RS00185PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA  [1] (data from MRSA252)
    SACOL_RS03075elongation factor G  [1] (data from MRSA252)
    SACOL_RS04805D-alanine--poly(phosphoribitol) ligase subunit 2  [1] (data from MRSA252)
    SACOL_RS1161530S ribosomal protein S9  [1] (data from MRSA252)
    SACOL_RS1168550S ribosomal protein L15  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.099
    • Ymax_pos: 38
    • Cmax: 0.122
    • Cmax_pos: 23
    • Smax: 0.138
    • Smax_pos: 3
    • Smean: 0.077
    • D: 0.09
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446981327 NCBI
  • RefSeq: WP_001058583 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFRKNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIVDGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQIRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYIETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSINDCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHEIAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQSDKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIVEIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFRVEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNEAQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHRTDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLIKVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08615 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]