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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08625 [old locus tag: SACOL1691 ]
  • pan locus tag?: SAUPAN004241000
  • symbol: SACOL_RS08625
  • pan gene symbol?: recJ
  • synonym:
  • product: single-stranded-DNA-specific exonuclease RecJ

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08625 [old locus tag: SACOL1691 ]
  • symbol: SACOL_RS08625
  • product: single-stranded-DNA-specific exonuclease RecJ
  • replicon: chromosome
  • strand: -
  • coordinates: 1721266..1723539
  • length: 2274
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1721266..1723539) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAA
    TTAACATCAAAATTAAAGTTAACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATT
    ATTGATGAACAAGCAATAGAATCCATCATTTCAGATACAGATATCAATCATGATGCACTG
    CAATTGAGCGATATGACTAAGACTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
    AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCGCAACTACAATTTTAGTG
    ATAACATTACAACTTTTAGGTGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAA
    GGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATGATGAAGGCATAACATTAATC
    ATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
    GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCA
    ATCGTACATCCAATGCATCCATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGT
    GTTGCATACAAATTAGCACAAGCATTGATTGAAAATGTACCTGATTATTTTAAAGCATTA
    GTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
    AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAA
    GAAGCGGGATACAATGACAATATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGA
    CTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTTGCGAATTGTTGATGACTGAC
    GTCGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
    GATATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAAG
    GGAGATTTATTTTTACTTTTAGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTT
    GCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAATTTTAAATATTGATAGGGAG
    CAAAATCATGCAAAAGGCTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
    TCAGCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACG
    ATGGATATTGAAAATATAGAATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTA
    TCTAAAACAACTTCGTTAGATCCCGTAAAGCAAGTAGATGTATTATTAACAGAAAATGAT
    ATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
    AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATAGGTCAACAA
    AAAAATCATCTAAAATTGACACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAAT
    GGACACTTAGAACCTGAGTTACAAGATGAACAGCCAATTAATATATTAGGATCTGTTCAA
    ATCAACGAATGGAATGGTAATCAATCACCGCAAATAATTATTCAAGATATTGCGATGAAT
    GAACAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGAT
    GAAAATATTGTCGTGCTTATTCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTAT
    TATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTATTAAGAGATTTACCAACGTCA
    ATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
    CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGT
    TATAAAGCATTAATAACTAAACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGT
    CAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGTTGAAAGTTTTCTTAGACTTA
    AAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
    ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTA
    TTATATCAAGATTTTTCAGAAATAAAAAATTGGATAAAGTCACAATTGTCGTGA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08625 [old locus tag: SACOL1691 ]
  • symbol: SACOL_RS08625
  • description: single-stranded-DNA-specific exonuclease RecJ
  • length: 757
  • theoretical pI: 4.90644
  • theoretical MW: 85610.4
  • GRAVY: -0.269485

Function[edit source | edit]

  • reaction:
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairsingle-stranded-DNA-specific exonuclease RecJ (TIGR00644; EC 3.1.-.-; HMM-score: 635.6)
  • TheSEED:
  • PFAM:
    no clan definedssDNA-exonuc_C; Single-strand DNA-specific exonuclease, C terminal domain (PF10141; HMM-score: 195.9)
    DHHA1; DHHA1 domain (PF02272; HMM-score: 76.8)
    HAD (CL0137) DHH; DHH family (PF01368; HMM-score: 46.4)
    Rep (CL0169) DUF1424; Putative rep protein (DUF1424) (PF07232; HMM-score: 10.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.129
    • Ymax_pos: 46
    • Cmax: 0.114
    • Cmax_pos: 46
    • Smax: 0.229
    • Smax_pos: 1
    • Smean: 0.111
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446517647 NCBI
  • RefSeq: WP_000594993 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVITLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLGVDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMAETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKQVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08625 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]