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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08645 [old locus tag: SACOL1695 ]
  • pan locus tag?: SAUPAN004246000
  • symbol: SACOL_RS08645
  • pan gene symbol?: queA
  • synonym:
  • product: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08645 [old locus tag: SACOL1695 ]
  • symbol: SACOL_RS08645
  • product: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA
  • replicon: chromosome
  • strand: -
  • coordinates: 1727737..1728762
  • length: 1026
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1727737..1728762) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    GTGAATATTGAAGAATTTGACTATGACTTACCAGAATCATTAATTGCTCAAACGCCTTTA
    AAAGATCGTGATCATAGTCGTTTATTAGTCATGGATAGAGAAACTGGTGAAATGAAACAT
    TTACATTTCAAAGATATCATTGAGTATTTTAGACCTGGTGATACATTAGTGCTTAACGAT
    ACGCGAGTAATGCCAGCTAGACTTTTTGGTTTAAAAGAAGAAACTGGTGCAAAAGTTGAA
    ATGTTAATGTTAACTCAAATTGAAGGTAATGATTGGGAAGTCTTACTGAAACCAGCTAAG
    CGTATTAAAGTTGGTAATAAATTGAATTTTGGTAATGGCAAAATTATAGCTGAATGCATA
    AAAGAAATGGATCAAGGTGGACGCATCATGCGTTTACATTATGAAGGTATTTTACAAGAA
    AGATTAGATGAATTAGGGGAAATGCCACTGCCACCATACATCAAAGAACGTTTAGATGAT
    CCAGATCGTTATCAAACAGTTTACGCTAAAGAAAGTGGTTCAGCGGCAGCACCAACAGCA
    GGATTACATTTTACTGATGAGTTATTAATTGAAATTAAAAATAAAGGTGTTAATATCGCA
    TTTGTTACATTACATGTTGGGTTAGGTACGTTTAGACCGGTGAGCGTAGACGATGTGAAT
    GACCACGAAATGCATAGTGAATATTATCAAATGACACAAGAAACAGCTGATTTATTAAAT
    GATACTAAGTCCAAAGGACATCGCATTATATCAGTTGGTACAACTTCAACACGTACACTT
    GAAACAATTCGACGCGATCATGATAAATTTGTTGAAACGAGTGGCTGGACTAATATATTT
    ATTTATCCAGGATTTGATTTTAAAGCAATTGATGGCCAGATTACTAATTTTCATTTACCA
    AAATCAACATTAGTTATGCTAGTATCAGCGTTTAGTAGTCGTGAAAATGTTCTGAATGCT
    TATAAAACGGCAGTAAATTTAGAATATAGATTCTTTAGTTTTGGCGATGCAATGTTAATT
    ATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
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    840
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    1020
    1026

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08645 [old locus tag: SACOL1695 ]
  • symbol: SACOL_RS08645
  • description: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA
  • length: 341
  • theoretical pI: 5.29781
  • theoretical MW: 38968.3
  • GRAVY: -0.343988

Function[edit source | edit]

  • reaction:
    EC 5.-.-.-?  ExPASy
    EC 2.4.99.17?  ExPASy
    S-adenosylmethionine:tRNA ribosyltransferase-isomeraseS-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine34 in tRNA = L-methionine + adenine + epoxyqueuosine34 in tRNA
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationS-adenosylmethionine:tRNA ribosyltransferase-isomerase (TIGR00113; EC 2.4.99.17; HMM-score: 476.2)
  • TheSEED:
  • PFAM:
    no clan definedQueuosine_synth; Queuosine biosynthesis protein (PF02547; HMM-score: 465.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.09
    • Ymax_pos: 44
    • Cmax: 0.103
    • Cmax_pos: 24
    • Smax: 0.121
    • Smax_pos: 23
    • Smean: 0.063
    • D: 0.079
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446941916 NCBI
  • RefSeq: WP_001019172 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDDPDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSFGDAMLII

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08645 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]