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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08795 [old locus tag: SACOL1722 ]
  • pan locus tag?: SAUPAN004291000
  • symbol: SACOL_RS08795
  • pan gene symbol?: tig
  • synonym:
  • product: trigger factor

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08795 [old locus tag: SACOL1722 ]
  • symbol: SACOL_RS08795
  • product: trigger factor
  • replicon: chromosome
  • strand: -
  • coordinates: 1753247..1754548
  • length: 1302
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1753247..1754548) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACAGCAACTTGGGAAAAAAAGGAAGGTAACGAAGGTTTATTAACTGTTACTGTTCCT
    GCAGAAAAAGTAAACAAAGCGTTAGATCAAGCATTCAAAAAAGTGGTTAAACAAATTAAC
    GTACCTGGATTCCGTAAAGGTAAAGTGCCACGTCCAATTTTTGAACAACGCTTTGGTGTA
    GAAGCATTATATCAAGATGCTATCGACATTTTATTACCAGATGCTTATGGTGAAGCAATT
    GACGAAACTGATATTAAACCAGTTGCACAACCAGAAGTAAGTGTTACTCAAATTGAAAAA
    GGTAAAGATTTCATTTTTGAAGCAACAGTTACAGTTGAGCCAGAAGTTAAATTAGGAGAC
    TATAAAGGTCTTGAAATTGAAAAACAAGAAACTGAATTATCTGATGATGAGTTACAAGAA
    GCGATTGACCACAGCTTAGGACATTTAGCTGAAATGGTCGTTAAAGAAGATGGTGTTGTT
    GAAAATGGCGACACAGTTAACATCGACTTTAGTGGTTCAGTTGACGGAGAAGAATTCGAA
    GGTGGACAAGCTGAAGGTTACGATTTAGAAATCGGTTCAGGTTCATTCATACCTGGTTTC
    GAAGAGCAATTAGAAGGTATGAAAGTTGACGAAGAAAAAGATGTTGTCGTAACATTCCCA
    GAAGAATACCATGCTGAAGAATTAGCCGGTAAAGAAGCAACTTTCAAAACAAAAGTTAAC
    GAAATTAAATTTAAAGAAGTCCCAGAATTAACAGATGAAATTGCTAATGAATTAGATGCA
    GAAGCAAATACAGTAGACGAGTACAAAGAAAACTTACGTAAACGTTTAGCTGAACAAAAA
    GCTACAGATGCTGAAAATGTTGAAAAAGAAGAAGCGATTACAAAAGCTACTGATAATACA
    ACAATCGATATTCCTGAAGCAATGGTTAATACTGAATTAGATCGTATGGTGTCTGAATTT
    GCACAAAGAATTCAACAACAAGGTTTAGATTTACAAACGTACTTCCAAATCTCAGGTCAA
    GATGAAACTCAATTAAGAGAGCAAATGAAAGACGATGCAGAACAACGTGTTAAAACTAAC
    TTAACTTTAACTGCGATCGCTGAAGCTGAAAAAATCGAAGCTACTGATGAAGATATCGAT
    AAAGAATTAGAAAAAATGAGTAAACAATTTAATATCTCAGTTGAAGATATCAAAAATACT
    TTAGGTAATACTGATATCATTAAAAATGATGTTCGTATCCAAAAAGTTATCGATTTATTA
    AGAGATAACGCAAAGTTCGTTGAAGGAACTAAAGAAGATTAA
    60
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    1302

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08795 [old locus tag: SACOL1722 ]
  • symbol: SACOL_RS08795
  • description: trigger factor
  • length: 433
  • theoretical pI: 4.05625
  • theoretical MW: 48608.6
  • GRAVY: -0.642956

Function[edit source | edit]

  • reaction:
    EC 5.2.1.8?  ExPASy
    Peptidylprolyl isomerasePeptidylproline (omega=180) = peptidylproline (omega=0)
  • TIGRFAM:
    Genetic information processingProtein fateProtein folding and stabilizationtrigger factor (TIGR00115; EC 5.2.1.8; HMM-score: 451.8)
    MetabolismFatty acid and phospholipid metabolismBiosynthesispoly(hydroxyalkanoate) granule-associated protein (TIGR01837; HMM-score: 8.2)
  • TheSEED:
  • PFAM:
    no clan definedTrigger_N; Bacterial trigger factor protein (TF) (PF05697; HMM-score: 156.9)
    Trigger_C (CL0262) Trigger_C; Bacterial trigger factor protein (TF) C-terminus (PF05698; HMM-score: 153.6)
    FKBP (CL0487) FKBP_C; FKBP-type peptidyl-prolyl cis-trans isomerase (PF00254; HMM-score: 57.6)
    HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 13.1)
    no clan definedDUF4854; Domain of unknown function (DUF4854) (PF16146; HMM-score: 8.5)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.123
    • Ymax_pos: 34
    • Cmax: 0.114
    • Cmax_pos: 24
    • Smax: 0.24
    • Smax_pos: 26
    • Smean: 0.104
    • D: 0.116
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446049718 NCBI
  • RefSeq: WP_000127573 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGFRKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGKDFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVVENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVTFPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRLAEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTYFQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNISVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08795 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]