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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08935 [old locus tag: SACOL1746 ]
  • pan locus tag?: SAUPAN004325000
  • symbol: SACOL_RS08935
  • pan gene symbol?: pfkA
  • synonym:
  • product: ATP-dependent 6-phosphofructokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08935 [old locus tag: SACOL1746 ]
  • symbol: SACOL_RS08935
  • product: ATP-dependent 6-phosphofructokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1783705..1784673
  • length: 969
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1783705..1784673) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGAAAATTGCAGTTTTAACTAGTGGTGGAGATTCACCTGGAATGAATGCTGCCGTA
    AGAGCAGTTGTTCGTACAGCAATTTACAATGAAATTGAAGTTTATGGTGTGTATCATGGT
    TACCAAGGATTGTTAAATGATGATATTCATAAACTTGAATTAGGATCAGTTGGGGATACG
    ATTCAGCGTGGAGGTACATTCTTGTATTCAGCAAGATGTCCAGAGTTTAAGGAGCAAGAA
    GTACGTAAAGTTGCAATCGAAAACTTACGTAAAAGAGGGATTGAGGGCCTTGTAGTTATT
    GGTGGTGACGGTAGTTATCGCGGTGCACAACGCATCAGTGAGGAATGTAAAGAAATTCAA
    ACTATCGGTATTCCTGGTACGATTGACAATGATATCAATGGTACTGATTTTACAATTGGA
    TTTGACACAGCATTAAATACGATTATTGGCTTAGTCGACAAAATTAGAGATACTGCGTCA
    AGTCACGCACGAACATTTATCATTGAAGCAATGGGCCGTGATTGTGGAGATCTAGCATTA
    TGGGCTGGATTATCAGTTGGTGCTGAGACAATTGTAGTTCCAGAAGTGAAAACAGATATT
    AAAGAAATAGCTGATAAAATTGAACAAGGTATTAAACGTGGTAAGAAACACTCAATCGTT
    CTTGTAGCAGAAGGTTGTATGACTGCGCAAGATTGTCAAAAAGAATTATCACAATACATC
    AATGTTGATAATAGAGTGTCTGTGTTAGGTCACGTTCAACGTGGTGGTAGCCCAACAGGT
    GCGGATAGAGTTTTAGCATCACGTTTAGGTGGATATGCGGTAGACTTATTAATGCAAGGT
    GAAACAGCTAAGGGTGTTGGAATTAAGAACAATAAAATTGTAGCAACATCTTTTGATGAA
    ATTTTTGATGGTAAAGATCATAAATTTGATTATAGTCTATATGAACTTGCTAACAAGTTA
    TCTATATAA
    60
    120
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    969

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08935 [old locus tag: SACOL1746 ]
  • symbol: SACOL_RS08935
  • description: ATP-dependent 6-phosphofructokinase
  • length: 322
  • theoretical pI: 5.71393
  • theoretical MW: 34839.4
  • GRAVY: -0.160559

Function[edit source | edit]

  • reaction:
    EC 2.7.1.11?  ExPASy
    6-phosphofructokinaseATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate
  • TIGRFAM:
    MetabolismEnergy metabolismGlycolysis/gluconeogenesis6-phosphofructokinase (TIGR02482; EC 2.7.1.11; HMM-score: 428)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphofructokinase (TIGR02483; HMM-score: 301.2)
    6-phosphofructokinase (TIGR02478; EC 2.7.1.11; HMM-score: 295.8)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisdiphosphate--fructose-6-phosphate 1-phosphotransferase (TIGR02477; EC 2.7.1.90; HMM-score: 82.2)
  • TheSEED:
  • PFAM:
    PFK (CL0240) PFK; Phosphofructokinase (PF00365; HMM-score: 367.1)
    NAD_kinase; ATP-NAD kinase (PF01513; HMM-score: 13.1)
    DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:
    SACOL_RS0303050S ribosomal protein L1  [1] (data from MRSA252)
    SACOL_RS03075elongation factor G  [1] (data from MRSA252)
    SACOL_RS03080elongation factor Tu  [1] (data from MRSA252)
    SACOL_RS04840NADH dehydrogenase  [1] (data from MRSA252)
    SACOL_RS05630pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SACOL_RS05635pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    SACOL_RS05640dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [1] (data from MRSA252)
    SACOL_RS05645dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    SACOL_RS0642050S ribosomal protein L19  [1] (data from MRSA252)
    SACOL_RS07385dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    SACOL_RS08930pyruvate kinase  [1] (data from MRSA252)
    SACOL_RS11435Asp23/Gls24 family envelope stress response protein  [1] (data from MRSA252)
    SACOL_RS1169530S ribosomal protein S5  [1] (data from MRSA252)
    SACOL_RS1174550S ribosomal protein L16  [1] (data from MRSA252)
    SACOL_RS1176550S ribosomal protein L2  [1] (data from MRSA252)
    SACOL_RS13725malate:quinone oxidoreductase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.173
    • Ymax_pos: 22
    • Cmax: 0.134
    • Cmax_pos: 22
    • Smax: 0.376
    • Smax_pos: 18
    • Smean: 0.219
    • D: 0.191
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446640215 NCBI
  • RefSeq: WP_000717561 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08935 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]