From AureoWiki
Jump to: navigation, search
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09130 [old locus tag: SACOL1783 ]
  • pan locus tag?: SAUPAN004397000
  • symbol: SACOL_RS09130
  • pan gene symbol?: acsA
  • synonym:
  • product: acetate--CoA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS09130 [old locus tag: SACOL1783 ]
  • symbol: SACOL_RS09130
  • product: acetate--CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1826506..1828212
  • length: 1707
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1826506..1828212) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAAGTCGAAGTTTATAAAGGAGCGCAAGGTAAACATAACCTTAAAGATTATGAAGAA
    ACATATAATACTTTTGATTGGAAAGACGTAGAACAAGCATTTTCTTGGAGTGAAACTGGA
    AAAATGAACATGGCATATGAATGCATAGATCGCCATGTAGATCAAGGATTAGGGGATAAA
    ATAGCGTTAAATTACAAAGATGAGCACAGAAAAGAATCGTATACTTATAAAGATATGCAA
    CGGTTATCTAATAAAGCAGCGAATGTTTTGTCTGAACATGCAGAAGTTGACAAAGGTGAC
    AGAGTATTTATATTTATGTCGCGTACACCTGAACTATATTTTGCGTTGTTAGGTGTTTTA
    AAAATTGGTGCAATTGTTGGGCCGTTATTTGAAGCATTTATGGAAAAGGCAGTTGCGGAT
    AGATTAGAGAACAGTGAAGCTAAAGTGTTAATTACTAATAAGGCATTGTTACCTCGAGTA
    CCTGTAGATAAATTACCAAACTTGAAAAAAATTGTTGTCGTAGATGAGGATGTAGAAGAC
    AATTACATAGACTTCATTAGTTTGATGGAAACTGCTAGCGATGAATTTGACATTGAATGG
    TTAAAGTCGGATGATGGTTTGATTTTACATTATACATCAGGTTCTACTAGGCAACCTAAA
    GGTGTATTGCATGTTCAACAAGCAATGTTAGTGCACTATATTTCTGGAAAATATGTATTA
    GATTTACAAGAAGATGATGTTTATTGGTGTACAGCAGATCCAGGTTGGGTTACAGGAACA
    TCTTATGGTATTTTTGCACCATGGTTAAATGGCGCTACAAATTGTATAGCTGGTGGTCGC
    TTTTCGCCAGAACAGTGGTATAGTATGATTGAAGATTTTAAAGTGACGATTTGGTATACG
    GCACCAACAGCTTTAAGAATGTTAATGAGTGCTGGTGACGATATTGTTGAGAAATATGAC
    TTGTCATCGTTACGTTCGATTCTATCAGTAGGTGAGCCTTTAAATCCTGAAGTTATAAAA
    TGGGCGAAAAAAGTATACGGTTTAACGGTGTTAGATACTTGGTGGATGACAGAAACAGGT
    GGACATATGATTGTTAACTATCCAACGATGGACGTCAAGCTTGGCTCAATGGGCAAACCA
    TTACCTGGTATTCAAGCTGCAATTATCGATGATGCAGGGAATGAATTACCACCAAATCGA
    ATGGGCAACCTTGCTATAAAAAAAGGCTGGCCATCAATGATGTATCGTATCTGGAAGAAT
    CCAGAAAAATATAAATCATATTTTATTGGAGACTGGTATGTATCTGGTGATTCGGCATAT
    AAAGATGAAGATGGTTACTTCTGGTTCCAAGGACGTGTTGATGATGTAATTATGACAGCT
    GGTGAACGAGTTGGACCATTTGAGGTTGAGTCTAAATTGGTTGAACACGAAGCAGTTGCC
    GAAGCAGGAATTATTGGTAAACCTGATCCGGTTCGCGGTGAAATAATTAAGGCGTTTGTT
    GCACTGAGAAAAGGATATGAACCAACAGACGAATTAAAAGAAGAAATTCGTATATTTGTT
    AAAGAAGGTTTGTCGGCACATGCAGCACCACGTGAAATCGAATTTAAAGATAAATTACCT
    AAAACACGGTCAGGTAAAATTATGAGACGTGTATTAAAAGCTTGGGAATTAAATTTAGAT
    GCTGGGGATTTAAGTACAATGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1707

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS09130 [old locus tag: SACOL1783 ]
  • symbol: SACOL_RS09130
  • description: acetate--CoA ligase
  • length: 568
  • theoretical pI: 4.85501
  • theoretical MW: 64463.2
  • GRAVY: -0.338908

Function[edit source | edit]

  • reaction:
    EC 6.2.1.1?  ExPASy
    Acetate--CoA ligaseATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
  • TIGRFAM:
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 538.9)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 302.4)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 283.6)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 195.4)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 184.2)
    amino acid adenylation domain (TIGR01733; HMM-score: 168.8)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 153.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneO-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 152.5)
    MetabolismTransport and binding proteinsCations and iron carrying compounds(2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 135.2)
    MetabolismCentral intermediary metabolismOtheracetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 131.1)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanD-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 93.1)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 50.6)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 32.2)
  • TheSEED:
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 310.3)
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 82.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 70
    • Cmax: 0.134
    • Cmax_pos: 46
    • Smax: 0.119
    • Smax_pos: 1
    • Smean: 0.078
    • D: 0.091
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

  • GI: 446784835 NCBI
  • RefSeq: WP_000862091 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNMAYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRVFIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRVPVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTRQPKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCIAGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPLNPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDAGNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQGRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEPTDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLSTME

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS09130 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]