From AureoWiki
Jump to: navigation, search
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09455 [old locus tag: SACOL1843 ]
  • pan locus tag?: SAUPAN004510000
  • symbol: SACOL_RS09455
  • pan gene symbol?: menC
  • synonym:
  • product: o-succinylbenzoate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS09455 [old locus tag: SACOL1843 ]
  • symbol: SACOL_RS09455
  • product: o-succinylbenzoate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1897263..1898264
  • length: 1002
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1897263..1898264) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTGACAGCTTTGCATTTTTATAAATATAGTGAGCCATTTAAGTCACAAATTGTA
    ACACCGAAAGTCACTTTAACGCATCGTGATTGTTTGTTTATCGAATTGATTGATGACAAA
    GGAAATGCATATTTCGGGGAATGTAACGCTTTTCAAACAGATTGGTATGATCATGAAACA
    ATTGCCTCAGTGAAACATGTAATTGAGCAATGGTTCGAAGATAATAGAAATAAATCATTT
    GAAACGTATGAAGCAGCACTAAAATTAGTAGATTCATTGGAAAATACGCCTGCTGCAAGG
    GCAACTATTGTCATGGCATTGTATCAAATGTTTCATGTACTGCCTTCATTTTCAGTAGCA
    TATGGAGCGACAGCGAGCGGCTTATCAAATAAACAACTAGAGTCATTAAAAGCAACAAAG
    CCGACCAGAATTAAATTAAAATGGACGCCTCAAATCATGCATCAAATTAGAGTGTTACGG
    GAATTAGACTTTCACTTTCAATTGGTTATAGATGCAAATGAGTCCTTGGATCGTCAAGAT
    TTCACACAGTTACAGTTATTAGCGCGTGAACAGGTTCTTTATATCGAAGAGCCATTTAAA
    GATATCTCCATGCTTGATGAAGTAGCGGATGGAACAATACCTCCAATAGCCCTTGATGAA
    AAGGCAACATCGTTGTTGGACATAATTAATTTGATAGAATTATACAATGTGAAAGTTGTT
    GTATTAAAACCATTTCGTCTAGGTGGCATTGATAAAGTGCAAACGGCAATTGATACTTTG
    AAAAGTCATGGTGCAAAAGTGGTTATTGGCGGCATGTACGAATATGGTTTAAGCCGCTAT
    TTTACAGCGATGCTTGCTCGTAAAGGTGATTATCCAGGAGATGTTACACCAGCAGGTTAT
    TATTTTGAACAAGATGTGGTGGCGCATTCAGGCATATTAAAAGAGGGGCGACTCGAATTT
    CGCCCCCCTTTAGTGGATATAACTCAATTACAACCATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

Protein[edit source | edit]

Protein Data Bank: 2OKT
Protein Data Bank: 2OLA

General[edit source | edit]

  • locus tag: SACOL_RS09455 [old locus tag: SACOL1843 ]
  • symbol: SACOL_RS09455
  • description: o-succinylbenzoate synthase
  • length: 333
  • theoretical pI: 5.62288
  • theoretical MW: 37967.3
  • GRAVY: -0.146847

Function[edit source | edit]

  • reaction:
    EC 4.2.1.113?  ExPASy
    o-succinylbenzoate synthase(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneo-succinylbenzoate synthase (TIGR01928; EC 4.2.1.113; HMM-score: 443.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneo-succinylbenzoate synthase (TIGR01927; EC 4.2.1.113; HMM-score: 82.3)
    muconate and chloromuconate cycloisomerases (TIGR02534; EC 5.5.1.-; HMM-score: 28.5)
    MetabolismAmino acid biosynthesisAspartate familyS-methyl-5-thioribose-1-phosphate isomerase (TIGR00512; EC 5.3.1.23; HMM-score: 12.1)
  • TheSEED:
  • PFAM:
    Enolase_TIM (CL0256) MR_MLE_C; Enolase C-terminal domain-like (PF13378; HMM-score: 55.7)
    MAAL_C; Methylaspartate ammonia-lyase C-terminus (PF07476; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: a divalent metal cation
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.156
    • Ymax_pos: 18
    • Cmax: 0.112
    • Cmax_pos: 44
    • Smax: 0.309
    • Smax_pos: 10
    • Smean: 0.227
    • D: 0.184
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446701177 NCBI
  • RefSeq: WP_000778523 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAYFGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARATIVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLRELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIALDEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYGLSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS09455 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]