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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09585 [old locus tag: SACOL1869 ]
  • pan locus tag?: SAUPAN004567000
  • symbol: SACOL_RS09585
  • pan gene symbol?: splA
  • synonym:
  • product: serine protease SplA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS09585 [old locus tag: SACOL1869 ]
  • symbol: SACOL_RS09585
  • product: serine protease SplA
  • replicon: chromosome
  • strand: -
  • coordinates: 1920785..1921501
  • length: 717
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1920785..1921501) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAATGTAATGGTTAAAGGTTTAACTGCTTTAACTATTTTAACTATTTTAACA
    TCTCTTGGTTTTGCTGAAAATATTTCTAATCAGCCTCATTCAATTGCCAAAGCAGAAAAG
    AATGTCAAAGAAATTACCGATGCAACTAAGGAACCATACAATTCAGTGGTAGCATTTGTG
    GGTGGTACTGGTGTAGTTGTTGGTAAAAATACAATCGTAACTAACAAACATATCGCTAAA
    AGTAATGATATTTTTAAAAATAGAGTATCAGCACATCATTCGAGTAAAGGTAAAGGCGGA
    GGAAACTACGACGTTAAAGACATTGTAGAATATCCCGGAAAAGAAGACCTTGCGATAGTT
    CATGTTCATGAAACAAGTACAGAAGGTTTGAATTTTAATAAGAACGTTAGTTATACAAAA
    TTTGCAGACGGAGCAAAAGTGAAAGATAGAATTTCTGTTATTGGTTATCCAAAGGGTGCA
    CAAACAAAATATAAAATGTTTGAATCGACAGGAACGATTAACCATATCAGTGGAACGTTT
    ATGGAATTTGATGCGTATGCACAACCAGGTAATTCAGGATCTCCTGTATTGAATTCTAAA
    CATGAACTGATTGGTATTTTATATGCAGGTAGTGGAAAAGATGAATCTGAAAAGAATTTC
    GGTGTTTATTTCACACCACAATTAAAAGAATTTATTCAAAATAATATTGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    717

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS09585 [old locus tag: SACOL1869 ]
  • symbol: SACOL_RS09585
  • description: serine protease SplA
  • length: 238
  • theoretical pI: 9.51102
  • theoretical MW: 25876.1
  • GRAVY: -0.393697

Function[edit source | edit]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 29)
    Signal transductionRegulatory functionsProtein interactionsperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 29)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.5)
    Genetic information processingProtein fateProtein folding and stabilizationpeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.5)
  • TheSEED:
  • PFAM:
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 73.8)
    Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 59.4)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 24.5)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 20.6)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 19.4)
    DUF31; Putative peptidase (DUF31) (PF01732; HMM-score: 17.3)
    Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide length 39 aa
    • Ymax: 0.404
    • Ymax_pos: 39
    • Cmax: 0.377
    • Cmax_pos: 39
    • Smax: 0.82
    • Smax_pos: 5
    • Smean: 0.655
    • D: 0.502
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446962167 NCBI
  • RefSeq: WP_001039423 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNKNVMVKGLTALTILTILTSLGFAENISNQPHSIAKAEKNVKEITDATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDVKDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTKYKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFGVYFTPQLKEFIQNNIEK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS09585 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]