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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS10300 [old locus tag: SACOL1970 ]
  • pan locus tag?: SAUPAN004939000
  • symbol: SACOL_RS10300
  • pan gene symbol?:
  • synonym:
  • product: staphopain A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS10300 [old locus tag: SACOL1970 ]
  • symbol: SACOL_RS10300
  • product: staphopain A
  • replicon: chromosome
  • strand: +
  • coordinates: 2034319..2035485
  • length: 1167
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2034319..2035485) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAAGAAACTTTCCAAAATTAATAGCTTTAAGTTTAATATTTTCTTTAAGTGTTACC
    CCTATTGCAAACGCTGAGAGCAATTCAAATATCAAAGCAAAAGATAAGAAGCATGTGCAA
    GTTAATGTCGAGGACAAGAGTGTTCCTACTGACGTACGTAATTTAGCGCAAAAAGACTAT
    CTTTCTTATGTTACTTCACTTGATAAAATCTATAATAAAGAGAAAGCCTCGTATACACTT
    GGTGAACCTTTTAAAATTTATAAATTCAACAAAAAGAGTGATGGTAATTATTATTTTCCA
    GTTTTGAACACTGAAGGTAATATCGATTATATAGTTACAATTTCACCAAAAATCACTAAG
    TATTCTAGTTCATCTTCTAAATACACAATCAATGTTTCTCCATTTTTATCTAAAGTCTTA
    AATCAATATAAAGATCAACAAATTACTATTTTGACAAACTCAAAAGGTTACTATGTGGTT
    ACTCAAAATCATAAAGCTAAACTAGTACTAAAAACACCACGTCTAGAAGATAAAAAGTTA
    AAAAAGACTGAGAGTATACCTACTGGCAATAATGTAACGCAACTAAAGCAAAAAGCTTCT
    GTCACTATGCCAACTAGTCAATTTAAAAGTAATAATTATACTTACAACGAGCAATATATA
    AATAAACTTGAGAATTTCAAGATAAGAGAAACTCAAGGTAACAATGGTTGGTGTGCAGGC
    TATACGATGTCTGAATTACTAAATGCGACTTATAATACTAACAAATATCATGCAGAAGCT
    GTGATGAGATTTTTACACCCAAACTTACAAGGACAACGATTCCAATTTACTGGTTTAACG
    CCAAGAGAGATGATTTATTTTGGACAAACTCAGGGAAGAAGTCCACAACTTCTTAATAGA
    ATGACTACATATAATGAAGTTGACAATCTTACTAAAAATAATAAAGGAATTGCTGTTTTA
    GGTAGTAGAGTAGAATCAAGAAATGGTATGCACGCAGGGCATGCCATGGCAGTTGTTGGG
    AACGCTAAATTAGATAATGGACAAGAGGTTATAATTATATGGAATTCATGGGATAATGGT
    TTTATGACTCAAGATGCTAAAAACAATGTTATTCCAGTATCCAATGGCGACCATTATCGA
    TGGTATTCATCTATTTATGGTTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1167

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS10300 [old locus tag: SACOL1970 ]
  • symbol: SACOL_RS10300
  • description: staphopain A
  • length: 388
  • theoretical pI: 10.0693
  • theoretical MW: 44251.9
  • GRAVY: -0.639948

Function[edit source | edit]

  • reaction:
    EC 3.4.22.48?  ExPASy
    StaphopainBroad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate
  • TIGRFAM:
    Genetic information processingTranscriptionRNA processingribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 10.2)
  • TheSEED:
  • PFAM:
    Peptidase_CA (CL0125) Peptidase_C47; Staphopain peptidase C47 (PF05543; HMM-score: 330.8)
    Cystatin (CL0121) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 284.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide length 26 aa
    • Ymax: 0.744
    • Ymax_pos: 26
    • Cmax: 0.609
    • Cmax_pos: 26
    • Smax: 0.942
    • Smax_pos: 16
    • Smean: 0.899
    • D: 0.804
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446750493 NCBI
  • RefSeq: WP_000827749 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNAKLDNGQEVIIIWNSWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS10300 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]