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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS11475 [old locus tag: SACOL2181 ]
  • pan locus tag?: SAUPAN005586000
  • symbol: SACOL_RS11475
  • pan gene symbol?: lacE
  • synonym:
  • product: PTS lactose EIICB component

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS11475 [old locus tag: SACOL2181 ]
  • symbol: SACOL_RS11475
  • product: PTS lactose EIICB component
  • replicon: chromosome
  • strand: -
  • coordinates: 2263934..2265646
  • length: 1713
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2263934..2265646) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
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    1021
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    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGCAAAAATTGATCGCACAAATTGAAAAAGGGAAGCCATTTTTCGAGAAATTATCA
    CGTAACATATATTTAAGAGCCATCCGTGATGGATTTATTTCTGCAATGCCTGTCATCTTA
    TTCTCAAGTATCTTTCTATTAATTGCATATGTGCCAAATATATTTGGTTTTAAATGGGAT
    AAAGGCATGGAAGCCATTTTAATGAAACCATATAACTATACGATGGGGCTTGTAGCATTT
    TTAGTAGCAGGAACGACAGCGAAATCTTTAACAGATTCATTTAACCGTAAGCTAGAAAGT
    ACAAATCAAATTAACTTTATTTCTACAATGCTAGCAGCGATGTGTGGATTCTTGTTCTTA
    GCTTCTGATCCAGCCAAAGATGGTGGATTCTTAAGTGCATTTATGGGAACTAAAGGTTTA
    TTAACAGCATTCTTATCAGCATTTGTAACTGTTATTGTTTATAACTTCTGTGTGAAGCGC
    AATATTACAATTAAAATGCCGAAAGAAGTACCGCCGAATATTTCACAAGTATTTAAGGAC
    TTAATTCCATTTTCAGCGGTAATCATCATTCTTTATGCATTAGATTTAGTCATTCGCAAC
    AGCTTTAAATCAAATGTAGCGGAAGGTATTTTAAAATTATTCGAACCATTATTTACAGCA
    GCAGATGGATGGATTGGTGTCACAATTATCTTTGGTGCCTTTGCATTATTCTGGTTTGTA
    GGTATTCATGGTCCGTCAATTGTAGAGCCAGCAATTGCAGCCATTACATATGCGAATATC
    GAAGCGAACTTCAAGTTGCTTCAAGCTGGAGAACACGCAGATAAAATTATTACATCAGGT
    ACACAAATGTTTATCGTTACATTTGGAGGTACGGGTGCAACATTGGTCGTCCCGTTCATG
    TTTATGTGGATGACGAAATCTAAACGAAACAAAGCGATTGGACGTGCATCAGTTGTACCA
    ACATTCTTTGGTGTAAACGAACCAATTTTATTTGGTGCACCACTAGTATTGAATCCTGTG
    TTCTTTATTCCATTTGTATTAGCACCAATTGTTAACGTATGGATTTTCAAACTTTTCGTT
    GAAGTGTTAGGAATGAATAGTTTTAGTGTGAATTTACCTTGGACAACACCAGGTCCATTG
    GGCATTATCATGGGTACAGGTTTTGGTTTATGGTCATTCGTACTAGCTATTACTTTGATT
    GTTGTAGATATTATTATTTACTACCCATTCCTAAAAGTTTATGATAGTGAAATTCTTGAT
    GAAGAAGAAGGACGTAAAGAAAGTAATTCAGATTTAAAAGAAAAAGTTGCAGCAAACTTT
    GATACGAAAAAAGCTGATTCAATTTTAGCGGCAAGTGGTGTATCAGACGATGCAGCTAAA
    GCTTCAAATATCACAGAACAAACAAATGTCTTAGTACTTTGTGCCGGTGGTGGTACAAGT
    GGATTATTAGCCAATGCACTTAATAAAGCAGCTGAAGAATATCATGTGCCAGTGAAAGCG
    GCAGCTGGCGGATATGGCGCACATATGGACATTATGAAAGAGTACCAATTAATCATTTTA
    GCGCCACAAGTTGCGTCTAACTATGAAGATATTAAGCAAGATACAGATAGATTAGGTATT
    AAATTAGCGAAGACTCAAGGTGCTGAATATATCAAATTGACACGAGATGGCCAAGCAGCC
    CTTGATTTCGTTCAACAACAATTTGAAAATTAA
    60
    120
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    240
    300
    360
    420
    480
    540
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    720
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    1140
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    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1713

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS11475 [old locus tag: SACOL2181 ]
  • symbol: SACOL_RS11475
  • description: PTS lactose EIICB component
  • length: 570
  • theoretical pI: 7.48602
  • theoretical MW: 62431.8
  • GRAVY: 0.442456

Function[edit source | edit]

  • reaction:
    EC 2.7.1.207?  ExPASy
    Protein-N(pi)-phosphohistidine--lactose phosphotransferase[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2)
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
    Signal transductionSignal transductionPTSPTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
    Signal transductionSignal transductionPTSPTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
    Signal transductionSignal transductionPTSPTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.3)
    Signal transductionSignal transductionPTSPTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.3)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 35.2)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 26.3)
  • TheSEED:
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 253)
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 40.7)
    PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 11.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 13
    • Cmax: 0.126
    • Cmax_pos: 58
    • Smax: 0.221
    • Smax_pos: 7
    • Smean: 0.149
    • D: 0.134
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_000983330 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKRNITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQQFEN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS11475 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]