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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS12215 [old locus tag: SACOL2323 ]
  • pan locus tag?: SAUPAN005817000
  • symbol: SACOL_RS12215
  • pan gene symbol?: hutI
  • synonym:
  • product: imidazolonepropionase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS12215 [old locus tag: SACOL2323 ]
  • symbol: SACOL_RS12215
  • product: imidazolonepropionase
  • replicon: chromosome
  • strand: -
  • coordinates: 2384501..2385739
  • length: 1239
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2384501..2385739) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
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    961
    1021
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    1141
    1201
    ATGAATGATTTAATAATTAATCATATAGCAGAATTAATTTTACCGAGATCAACAGATAAA
    CCTTTGAAGGGTAAGGAATTAGATGAATTAAATGTTGTGAAAAATGGTACAGTTGTCATT
    AAAGATGGCAAAATTGTATATGCTGGAACACATACAGATGACTACGATGCGACTGAGACG
    ATTGATGCTAGTGGGAAGGTAGTGTCCCCAGCATTAGTAGATGCACATACCCATTTAACA
    TTTGGTGGATCTCGAGAACATGAGATGTCATTAAAACGTCAAGGTAAGTCTTACCTTGAA
    ATATTAGAAATGGGTGGTGGCATATTATCTACCGTTAATGCTACTAGAGAAACATCCGAA
    GATGACTTGTTTAAAAAAGCAGAGCATGATTTGCTTACTATGATTAAACACGGCGTGCTT
    GCAGTTGAAAGTAAGAGTGGTTATGGATTAGATAGAGAAAATGAACTGAAGCAATTGAAA
    GTGTCTAATCGTTTAGCTGAGAAATATGATTTAGACATGAAACATACTTTCCTAGGGCCT
    CATGCTGTACCTAAAGAGGCAAGTTCAAATGAGGCATTTTTAGAAGAAATGATTGCGTTA
    CTTCCGGAAGTAAAACAATATGCAGACTTTGCGGATATTTTCTGTGAAACAGGTGTATTT
    ACAATAGAACAATCGCAACATTATATGCAAAAAGCCAAAGAAGCAGGTTTTAAAGTGAAA
    ATACATGCGGATGAAATTGATCCGTTAGGCGGACTGGAATTAGCAATTGATGAACAAGCA
    ATATCAGCAGACCACTTAGTAGCTTCTAGTGATAAAGGAAAAGAAAAGTTGCGAAATAGT
    GATACTGTAGCTGTCCTCTTACCTGCAACGACGTTCTATTTAGGTAAAGAAGATTATGCA
    GATGCAAGAGGCATGCTTGACAATAACGGTGCGATTGCATTAGCAACTGATTATAACCCT
    GGTAGTAGTGTCACAAACAACTTACAACTTGTTATGGCGATTGCAGCCTTGAAATTAAAG
    CTATCACCTAATGAAGTTTGGAATGCTGTTACGGTCAATGCTGCTAAAGCAATAGACATT
    AACGCGGGTACAATTAACACAGGTGATAAGGCTAATTTAGTAATTTGGGATGCACCAAAT
    CATGAATATATTCCATATCATTTTGGTATCAATCACGCAGAAAAAGTTATTAAAGACGGT
    AAGGTCATCGTAGATAACACAGTATCTTTTAAAGCATAA
    60
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    1020
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    1239

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS12215 [old locus tag: SACOL2323 ]
  • symbol: SACOL_RS12215
  • description: imidazolonepropionase
  • length: 412
  • theoretical pI: 5.03086
  • theoretical MW: 45038.7
  • GRAVY: -0.258738

Function[edit source | edit]

  • reaction:
    EC 3.5.2.7?  ExPASy
    Imidazolonepropionase(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesimidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 434.1)
    MetabolismEnergy metabolismOtherphosphonate metabolism protein PhnM (TIGR02318; HMM-score: 45.5)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotidesadenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 38.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesOtherguanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 37.3)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 36.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisdihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 35.9)
    MetabolismCentral intermediary metabolismAmino sugarsN-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 35.7)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 33.1)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 33)
    Hypothetical proteinsConservedputative amidohydrolase, EF_0837/AHA_3915 family (TIGR03583; HMM-score: 32.3)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 26.3)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesbeta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 18.8)
  • TheSEED:
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 72.2)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 60.4)
    Phage_TACs (CL0567) Phage_TAC_6; Phage tail assembly chaperone protein, TAC (PF09550; HMM-score: 14.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Fe2+, Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.132
    • Ymax_pos: 25
    • Cmax: 0.12
    • Cmax_pos: 25
    • Smax: 0.211
    • Smax_pos: 20
    • Smean: 0.13
    • D: 0.131
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446921511 NCBI
  • RefSeq: WP_000998767 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGKIVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEILEMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLKVSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETGVFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEKLRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAIAALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINHAEKVIKDGKVIVDNTVSFKA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS12215 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]