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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS12735 [old locus tag: SACOL2427 ]
  • pan locus tag?: SAUPAN005966000
  • symbol: SACOL_RS12735
  • pan gene symbol?: bioA
  • synonym:
  • product: adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS12735 [old locus tag: SACOL2427 ]
  • symbol: SACOL_RS12735
  • product: adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA
  • replicon: chromosome
  • strand: -
  • coordinates: 2485322..2486680
  • length: 1359
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2485322..2486680) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    1021
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    1141
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    1261
    1321
    ATGAATTACACACAACAACTTAAACAAAAAGACTCAGAATATGTTTGGCATCCATTTACA
    CAAATGGGTGTATATAGCAAAGAAGAAGCAATCATCATTGAAAAAGGAAAGGGTAGTTAC
    CTTTACGATACGAATGGCAATAAATATTTAGATGGTTATGCATCGTTGTGGGTCAATGTG
    CATGGTCATAATAACAAATACTTAAATAAGGTAATTAAAAAGCAACTCAATAAAATTGCC
    CATTCTACGCTGCTAGGATCATCAAATATTCCGTCAATAGAACTTGCGGAAAAATTAATC
    GAAATCACGCCAAGTAATCTAAGAAAAGTATTTTATTCTGATACAGGCAGTGCGTCTGTT
    GAAATCGCAATAAAGATGGCATATCAGTATTGGAAAAATATTGATAGAGAAAAATATGCC
    AAGAAAAACAAGTTTATAACGCTAAATCACGGTTATCATGGGGATACGATTGGTGCGGTA
    AGTGTTGGTGGTATCAAGACCTTTCATAAAATATTTAAAGACTTAATATTTGAGAATATT
    CAAGTAGAAAGCCCATCTTTCTATCGCAGTAATTACGATACTGAAAATGAAATGATGACA
    GCTATTTTAACGAATATAGAGCAAATTCTAATTGAAAGAAATGATGAAATCGCAGGGTTT
    ATATTGGAACCGTTGATTCAAGGTGCGACAGGCTTGTTTGTTCATCCTAAAGGCTTTTTG
    AAAGAAGTCGAGAAATTGTGCAAAAAATACGATGTCTTATTAATTTGTGATGAGGTAGCA
    GTTGGTTTTGGGAGAACTGGAAAGATGTTTGCATGCAATCATGAAGATGTTCAACCGGAT
    ATTATGTGTTTAGGTAAGGCGATTACTGGTGGCTACTTACCACTTGCAGCTACATTGACA
    TCTAAAAAAATATACAATGCATTTTTAAGTGATTCGCATGGTGTGAATACCTTTTTCCAT
    GGTCATACATACACCGGAAATCAAATCGTTTGTACGGTTGCATTAGAAAATATAAGACTT
    TATGAAAAACGTAAGTTATTGTCACATATTGAAACGACATCATCAACACTTGAGAAACAG
    TTACATGCGCTGAAGCGTCATCGAAATGTTGGTGATGTAAGAGGACGAGGCTTAATGTTT
    GGAGTTGAATTAGTTACAGATAAAGATAGTAAAACGCCGTTAGAAATTGAAAAAGTTGAA
    CGTATTGTACGTAATTGTAAAGAAAACGGGCTAATGATTAGAAATTTAGAAAATGTCATT
    ACGTTTGTGCCAGTGTTAAGTATGTCAAATAAAGAAGTGAAAACGATGGTACGTATTTTT
    AAAAAGGCAGTACATAACATTTTAGATAGGAAGTGTTAA
    60
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    1359

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS12735 [old locus tag: SACOL2427 ]
  • symbol: SACOL_RS12735
  • description: adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA
  • length: 452
  • theoretical pI: 9.20767
  • theoretical MW: 51404
  • GRAVY: -0.287389

Function[edit source | edit]

  • reaction:
    EC 2.6.1.62?  ExPASy
    Adenosylmethionine--8-amino-7-oxononanoate transaminaseS-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinadenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 550.6)
    transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 269.6)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 226.6)
    MetabolismCentral intermediary metabolismOther4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 202.2)
    MetabolismEnergy metabolismAmino acids and aminessuccinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 201.7)
    MetabolismCentral intermediary metabolismOther2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 192.1)
    MetabolismCentral intermediary metabolismPolyamine biosynthesisputrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 189.2)
    Cellular processesCellular processesAdaptations to atypical conditionsdiaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 168.4)
    L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 144.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminglutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 128.6)
    MetabolismCentral intermediary metabolismOther4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 99)
    MetabolismEnergy metabolismAmino acids and aminesglycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 12.5)
  • TheSEED:
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 386.2)
    no clan definedPox_L5; Poxvirus L5 protein family (PF04872; HMM-score: 12.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 39
    • Cmax: 0.113
    • Cmax_pos: 31
    • Smax: 0.184
    • Smax_pos: 35
    • Smean: 0.112
    • D: 0.113
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447032808 NCBI
  • RefSeq: WP_001110064 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAVSVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS12735 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]