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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00304
  • pan locus tag?: SAUPAN001855000
  • symbol: SAOUHSC_00304
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00304
  • symbol: SAOUHSC_00304
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 319041..320042
  • length: 1002
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGGTTAAATTAAGCGTATTAGACTATGCCTTAATAGATGAAGGTAAGGATGCACAAAAG
    GCATTGCAAGATTCAGTGACACTTGCAAAATTAGCAGATCGACTTGGCTTTAAGCGAATT
    TGGTTTACGGAACATCATAATGTACCAGCGTTTGCGTGTAGTAGTCCAGAACTTTTGATG
    ATGCATACATTGGCGCAGACAAATCACATACGAGTTGGCTCTGGTGGTGTGATGCTGCCG
    CACTATCGACCTTATAAAATTGCTGAGCATTTTAGAATGATGGCAGCGTTATATCCAAAT
    CGTATTGATTTAGGTATTGGTAATAATCCAGGTACTACTATGGTAAAGCAAGCTTTAGAT
    GGAATAAATCCTACATATGATAGTTACGATGAATCGATTTCGTTATTACGTGATTATCTT
    ACAATAAAGGATAAACCAAGTGCGCATACGTTAGGTGTCCAACCACACATTGATCATTTT
    CCAGAAATGTGGTTATTAAGTAGTAGCGCAACATCTGCCAAAATAGCTGCCGAACTAGGT
    ATAGGGCTTTCTGTTGGAACATTTTTGCTACCAGATATAAATGCGATACATACAGCGAAG
    GATAACATTGATATTTACAAAAAACATTTCCAAGCATCAACGATTAAAATGGACGCAAAG
    GTGATGGCATCTGTATTTGTCATTGTAGCTGATAACGAAGCGGAAGTAGCAGCATTACAA
    CATGCCTTAGATGTTTGGTTATTAGGTAAATTACAATTTGCAGAATTTGAAGATTTTCCT
    TCAGTAGACACAGCACAAAAGTATAAGCTTAATGATCGAGACAAAGAGATGATTCAAGCA
    CATCAAGCACGCATCATTGCAGGTACACAAGAAAAGGTTAAAGCACAATTAGATGATTTC
    ATTGCTACGTTTGAAGTTGATGAGGTGTTAGTAGCACCGCTTATTCCAGGTATTGAACAG
    CGTTGTAAAACATTAAAATTACTCGCGGAAATTTATTTGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00304
  • symbol: SAOUHSC_00304
  • description: hypothetical protein
  • length: 333
  • theoretical pI: 6.18693
  • theoretical MW: 37099.6
  • GRAVY: -0.0393393

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityluciferase family oxidoreductase, group 1 (TIGR03558; EC 1.-.-.-; HMM-score: 349.6)
    Unknown functionEnzymes of unknown specificityputative FMN-dependent luciferase-like monooxygenase, KPN_01858 family (TIGR04027; EC 1.-.-.-; HMM-score: 48.4)
    natural product biosynthesis luciferase-like monooxygenase domain (TIGR04020; EC 1.-.-.-; HMM-score: 42.9)
    Unknown functionEnzymes of unknown specificityputative luciferase-like monooxygenase, FMN-dependent, CE1758 family (TIGR04036; EC 1.-.-.-; HMM-score: 41.2)
    probable oxidoreductase, LLM family (TIGR03858; EC 1.-.-.-; HMM-score: 37.6)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, MSMEG_3544 family (TIGR03854; HMM-score: 33.9)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, Rv3093c family (TIGR03841; EC 1.-.-.-; HMM-score: 33.3)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, MSMEG_4141 family (TIGR03620; HMM-score: 28.5)
    TAT-translocated F420-dependent dehydrogenase, FGD2 family (TIGR04465; EC 1.-.-.-; HMM-score: 26.5)
    Unknown functionEnzymes of unknown specificityF420-dependent oxidoreductase, MSMEG_4879 family (TIGR03564; EC 1.-.-.-; HMM-score: 25.9)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, Rv2161c family (TIGR03619; EC 1.-.-.-; HMM-score: 25.2)
    MetabolismEnergy metabolismMethanogenesis5,10-methylenetetrahydromethanopterin reductase (TIGR03555; EC 1.5.98.2; HMM-score: 24.8)
    MetabolismEnergy metabolismPentose phosphate pathwayglucose-6-phosphate dehydrogenase (coenzyme-F420) (TIGR03554; EC 1.1.98.2; HMM-score: 22.9)
    Unknown functionEnzymes of unknown specificityluciferase-type oxidoreductase, BA3436 family (TIGR03571; EC 1.-.-.-; HMM-score: 22.3)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, MSMEG_2249 family (TIGR03857; HMM-score: 20.8)
    Unknown functionEnzymes of unknown specificityF420-dependent oxidoreductase, CPS_4043 family (TIGR03842; HMM-score: 18.3)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, MSMEG_2516 family (TIGR03621; HMM-score: 16.7)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, Rv3520c family (TIGR03559; HMM-score: 14.4)
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, Rv1855c family (TIGR03560; HMM-score: 13.9)
    Unknown functionEnzymes of unknown specificitycoenzyme F420-dependent oxidoreductase, NP1902A family (TIGR04024; EC 1.14.-.-; HMM-score: 12.4)
    Unknown functionEnzymes of unknown specificityFMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family (TIGR03860; EC 1.-.-.-; HMM-score: 12.1)
  • TheSEED:  
    Luciferase-like monooxygenase (EC 1.14.-.-) 
  • PFAM:
    no clan definedBac_luciferase; Luciferase-like monooxygenase (PF00296; HMM-score: 105.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 21
    • Cmax: 0.141
    • Cmax_pos: 53
    • Smax: 0.178
    • Smax_pos: 18
    • Smean: 0.103
    • D: 0.113
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTEHHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRIDLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHFPEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHTAKDNIDIYKKHFQASTIKMDAKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKEMIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]